Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,057,643	T→C	K256K (AAA→AAG)	serA ←	phosphoglycerate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,057,643	0	T	C	100.0%	175.3 / NA	49	K256K (AAA→AAG)	serA	phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/26);  total (23/26)

TTCCTGAGTCGAACCGCCAATGTGTGGCGTCAGAAGGACGTTGTCGAATTCACACAGCGGAGAGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGTTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCATTAGTGAAATTTCTTTCGCGCCCATCATATTTTTGGTGGACGGATTCTCTGG  >  NC_000913/3057514‑3057777                                                                                                                                  |                                                                                                                                       ttCCTGAGTCGAACCGCCAATGGGTCGCGTCAGAAGGGCGTTGTCGAATTCACACAGCGGAGAGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCa                                                                                                                              <  1:320713/140‑1 (MQ=255)           gAACCGCCAATGTGTGGCGTCAGAAGGACGTTGTCGAATTCACACAGCGGAGAGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATcaca                                                                                                                    >  2:329/1‑140 (MQ=255)             aCCGCCAATGTGTGGCGTCAGAAGGACGTTGTCGAATTCACACAGCGGAGAGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATcacaca                                                                                                                  >  2:552092/1‑140 (MQ=255)                gCCAATGTGTGGCGTCAGAAGGACGTTGTCGAATTCACACAGCGGAGAGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAgcg                                                                                                               <  2:479067/140‑1 (MQ=255)                   aaTGTGTGGCGTCAGAAGGACGTTGTCGAATTCACACAGCGGAGAGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCg                                                                                                            >  2:814037/1‑140 (MQ=255)                     tgtgtgGCGTCAGAAGGACGTTGTCGAATTCACACAGCGGAGAGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGa                                                                                                          <  1:580768/140‑1 (MQ=255)                     tgtgtgGCGTCAGAAGGACGTTGTCGAATTCACACAGCGGAGAGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGa                                                                                                          <  1:329/140‑1 (MQ=255)                     tgtgcgGCGTCAGAAGGACGTTGTCGAATTCACACAGCGGAGAGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGa                                                                                                          <  2:743236/140‑1 (MQ=255)                        gtgGCGTCAGAAGGACGTTGTCGAATTCACACAGCGGAGAGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAata                                                                                                       <  1:664087/140‑1 (MQ=255)                          ggCGTCAGAAGGACGTTTTCGAATTCACACACCGGAGAGTTAAATGGACCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATc                                                                                                     <  2:280565/140‑1 (MQ=255)                          ggCGTCAGAAGGACGTTGTCGAATTCACACAGCGGAGAGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATc                                                                                                     <  2:730003/140‑1 (MQ=255)                          ggCGTCAGAAGGACGTTGTCGAATTCACACAGCGGAGAGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATc                                                                                                     <  1:742742/140‑1 (MQ=255)                          ggCGTCAGAAGGACGTTGTCGAATTCACACAGCGGAGAGGTAAATGGAACGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATc                                                                                                     <  1:636602/140‑1 (MQ=255)                          ggCGTCAGAAGGACGCTGTCGACTTAACACAGCGGAGTGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATc                                                                                                     <  1:247142/140‑1 (MQ=255)                            cGTCAGAAGGACGTTGTCGAATTCACACAGTGGAGAGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATcca                                                                                                   <  2:725737/140‑1 (MQ=255)                            cGTCAGAAGGACGTTCTCGAATTCACACACCGGAGAGATAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATcca                                                                                                   <  1:233956/140‑1 (MQ=255)                               cAGAAGGACGTTGTCGAATTCACACAGCGGAGAGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATccacca                                                                                                >  2:480512/1‑140 (MQ=255)                               cAGAAGGACGTTGTCGAATTCACACAGCGGAGAGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCACGCGAGCGCATCGCACAGCGCCGGAATATccacca                                                                                                >  2:254092/1‑140 (MQ=255)                                            tCGAATTCACACAGCGGAGAGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGa                                                                                   >  1:48429/1‑140 (MQ=255)                                               aaTTCACACAGCGGAGAGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGc                                                                                >  2:711686/1‑140 (MQ=255)                                                                    aaTGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCggg                                                           >  2:564971/1‑140 (MQ=255)                                                                    aaTGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCggg                                                           >  1:65889/1‑140 (MQ=255)                                                                         aTCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCa                                                      >  1:833270/1‑140 (MQ=255)                                                                         aTCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCa                                                      >  1:133167/1‑140 (MQ=255)                                                                           cGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCAtt                                                    >  1:62854/1‑140 (MQ=255)                                                                           cGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCAtt                                                    <  1:480512/140‑1 (MQ=255)                                                                           cGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCAtt                                                    >  2:174234/1‑140 (MQ=255)                                                                           cGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCAtt                                                    >  1:29932/1‑140 (MQ=255)                                                                                ttGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCggg                                                           >  1:131601/1‑128 (MQ=255)                                                                                ttGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCggg                                                           <  2:131601/128‑1 (MQ=255)                                                                                ttGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCggg                                                           <  2:472488/128‑1 (MQ=255)                                                                                ttGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCggg                                                           >  1:472488/1‑128 (MQ=255)                                                                                ttGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCATTAGTGa                                               <  1:814037/140‑1 (MQ=255)                                                                                   gTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCATTAGTGAAAt                                            >  2:99271/1‑140 (MQ=255)                                                                                     cGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTAGCGCGCGAAGCATCAATAAGCAGCGATCCGGGCTTCATTAGTGAAAttt                                          >  2:236206/1‑140 (MQ=255)                                                                                     cGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCATTAGTGAAAttt                                          >  2:66669/1‑140 (MQ=255)                                                                                        cGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCATTAGTGAAAtttctt                                       >  2:508593/1‑140 (MQ=255)                                                                                        cGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCATTAGTGAAAtttctt                                       >  1:589309/1‑140 (MQ=255)                                                                                           ttCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCATTAGTGAAATTTCTTTcg                                    <  2:660969/140‑1 (MQ=255)                                                                                              cGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCAGGGTTTTATTAGTTAAATTTCTTTCgcgc                                 >  1:272317/1‑140 (MQ=255)                                                                                               gTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCATTAGTGAAATTTCTTTCGCGcc                                <  1:564971/140‑1 (MQ=255)                                                                                                  gggAATACGTCGATTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCATTAGTGAAATTTCTTTCGCGCCcat                             <  1:569041/140‑1 (MQ=255)                                                                                                  gggAATACGTCGATTGCAGCCCCCGCCAGATCCTTGCTCCCCAGCGCAACACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCATTAGTGAAATTTCTTTCGCGCCcat                             <  2:272317/140‑1 (MQ=255)                                                                                                              aTTGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCATTAGTGAAATTTCTTTCGCGCCCATCATATTTTtggt                 >  2:539575/1‑140 (MQ=255)                                                                                                               ttGCCGCCCCCGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCATTAGTGAAATTTCTTTCGCGCCCATCATATTTTtggtg                <  1:539575/140‑1 (MQ=255)                                                                                                                     cccccGCCAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCATTAGTGAAATTTCTTTCGCGCCCATCATATTTTTGGTGGACGGa          <  1:711686/140‑1 (MQ=255)                                                                                                                            cAGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCATTAGTGAAATTTCTTTCGCGCCCATCATATTTTTGGTGGACGGATTCTCTg   <  2:133167/140‑1 (MQ=255)                                                                                                                             aGATGCTTGCTCGCCAGCGCATTACACAGCGCCGGAAGATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCATTAGTGAAATTTCTTTCGCGCCCATCATATTTTTGGTGGACGGATTCTCTgg  <  1:317469/140‑1 (MQ=255)                                                                                                                             aGATGCTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCATTAGTGAAATTTCTTTCGCGCCCATCATATTTTTGGTGGACGGATTCTCTgg  <  1:66669/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       TTCCTGAGTCGAACCGCCAATGTGTGGCGTCAGAAGGACGTTGTCGAATTCACACAGCGGAGAGGTAAATGGATCGCTATTGGTCGCCGGTTCCGTCGGGAATACGTCGATTGCCGCCCCCGCCAGATGTTTGCTCGCCAGCGCATCACACAGCGCCGGAATATCCACCACAGTACCGCGCGAAGCATTAATCAGCAGCGAGCCGGGCTTCATTAGTGAAATTTCTTTCGCGCCCATCATATTTTTGGTGGACGGATTCTCTGG  >  NC_000913/3057514‑3057777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑