| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,066,127 | C→T | A442A (GCC→GCT) | scpC → | propionyl‑CoA:succinate CoA transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,066,127 | 0 | C | T | 98.0% | 157.5 / NA | 49 | A442A (GCC→GCT) | scpC | propionyl‑CoA:succinate CoA transferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (22/26); minor base G (0/1); total (22/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCCCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAAT > NC_000913/3065990‑3066255 | tCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCg > 2:575625/1‑140 (MQ=255)tCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCCCCGATCTACGCAGTCTTTCCCAGCTTCAACGCGCTCg > 1:417015/1‑140 (MQ=255) gATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCa < 1:69352/140‑1 (MQ=255) tGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTa < 2:462481/140‑1 (MQ=255) cTAAAGAAGGCAAGACCTCAACCGTCGTGCCAATGTGTAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATc < 1:353754/140‑1 (MQ=255) gaagGCAATATCTCAATAGTCGTGCCAATGGTCTGACATGTTGATCACCGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGa < 2:417015/140‑1 (MQ=255) ggCAAGATCTGAGCGGACGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATATGCGCGGTCGTTCCCCGCTTCAACGCGCGCGCACTATCATTGATaa < 2:63399/140‑1 (MQ=255) acaggATCTCAACCGTCGTGCCCATCTGCAGCCATGTCGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAAt < 1:227407/136‑1 (MQ=255) gATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATtgtg < 1:47684/140‑1 (MQ=255) gATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATtgtg < 1:575625/140‑1 (MQ=255) gATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATtgtg > 1:634118/1‑140 (MQ=255) aTCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGc < 1:58947/140‑1 (MQ=255) tctcAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGca < 1:675592/140‑1 (MQ=255) aaCCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATaa < 2:827737/129‑1 (MQ=255) aaCCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATaa > 1:827737/1‑129 (MQ=255) aaCCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATc > 1:671902/1‑140 (MQ=255) aCCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTCCCCCGCTTCAACGCGCTCGCACTATCA‑TGATAATTGTGCACATCCt > 2:193273/1‑140 (MQ=255) tcgtGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCGCCGCTTCAGCGCGCTCTCACTATCAGTGATAATTGTGCACATccctgt > 2:227407/1‑136 (MQ=255) tcgtGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAAt > 1:2081/1‑125 (MQ=255) tcgtGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAAt < 2:2081/125‑1 (MQ=255) ccAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCg > 2:580941/1‑140 (MQ=255) cAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATc > 2:582500/1‑140 (MQ=255) cAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATc > 2:435794/1‑140 (MQ=255) aTCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTAt > 2:193111/1‑140 (MQ=255) aGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGa > 1:787910/1‑140 (MQ=255) aGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGa > 1:167432/1‑140 (MQ=255) gCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGaa > 1:149546/1‑140 (MQ=255) cGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGaaa > 2:31427/1‑140 (MQ=255) gCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCt > 2:97780/1‑140 (MQ=255) gCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCt < 1:757519/140‑1 (MQ=255) gTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTgg < 2:671902/140‑1 (MQ=255) aaGTGATCATCACCGTACAAGGAATCGCCCATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGa < 1:263014/140‑1 (MQ=255) tcatcaCCGAACAAGGGATCGCTGATCTGCGGGGTATTTCCCCGCTTCCACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATAtt < 2:342998/140‑1 (MQ=255) aCCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTcacca < 2:167432/140‑1 (MQ=255) aCCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTcacca < 2:634118/140‑1 (MQ=255) aCCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTcacca < 2:149546/140‑1 (MQ=255) aCCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTcacca < 1:435794/140‑1 (MQ=255) aCCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTcacca < 1:582500/140‑1 (MQ=255) gAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGa < 2:64886/140‑1 (MQ=255) gggATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAg > 2:173248/1‑140 (MQ=255) gggATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAg > 2:546969/1‑140 (MQ=255) gggATCGCCGATCTGCGCGGTCTTTCCCCGCTCCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAg > 1:713595/1‑140 (MQ=255) gATCGCCGATCTGCGCGTTCTTACCCCGCTTTAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGcc < 2:317088/140‑1 (MQ=255) gCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGt > 2:725789/1‑140 (MQ=255) gATCTGCGCGGTCTTCCCCCGCTTCAACGCGCTCGCACTATCA‑TGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTc < 1:193273/140‑1 (MQ=255) tGCGCGGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGAc < 2:585456/140‑1 (MQ=255) cgcgGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCt > 2:173426/1‑133 (MQ=255) cgcgGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCt < 1:173426/133‑1 (MQ=255) gcgGTCTTTCCCCGCTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTa < 1:580941/140‑1 (MQ=255) cTTCAACGCGCTCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGtaatttaat > 2:382502/1‑140 (MQ=255) | TCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCCCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAAT > NC_000913/3065990‑3066255 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |