Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,107,477	T→C	D560G (GAT→GGT)	speC ←	ornithine decarboxylase, biosynthetic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,107,477	0	T	C	100.0%	156.7 / NA	44	D560G (GAT→GGT)	speC	ornithine decarboxylase, biosynthetic
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/20);  total (24/20)

TGTAGGTCTTTGACGTCGAAACTGACATACAGATCGTGCATCTCCTGACACAACTGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCATCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATAAAAACAGAATGGAGTTGAGATCGCACTTCTCCGGCACAATGCCGTTCTCACGCAGA  >  NC_000913/3107345‑3107611                                                                                                                                     |                                                                                                                                       tGTAGGTCTTTGACGTCGAAACTGACATACATATCTTGCCTCTCCTGACACAACTGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAat                                                                                                                                 <  2:335412/140‑1 (MQ=255)   gtggTCTTTGACGTCGACACTGTCATACAGAGCGTTCATCTCCTGACATAACTGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAatat                                                                                                                               <  2:314780/138‑1 (MQ=255)           tGACGTCGAAACTGACATACAGATTGTGCATCTCCTGACACATCTGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCa                                                                                                                       >  2:49616/1‑140 (MQ=255)                     aCTGACATACAGATCGTGCATCTCCTGACACAACTGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCa                                                                                                             <  2:686307/140‑1 (MQ=255)                     aCTGACATACAGATCGTGCATCTCCTGACACAACTGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCa                                                                                                             <  2:679305/140‑1 (MQ=255)                          agacaggATCGTGCATTCCCTGTCACAACTGTCCCCGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATc                                                                                                        <  2:416670/134‑1 (MQ=255)                              cAGATCGTGCATCTCCTGACACCACCGGCGCAGGGTGCAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGcca                                                                                                    <  1:237978/140‑1 (MQ=255)                                   cGTGCATCTCCTGACACAACTGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGGGAGTCACCCTCAATATTCTGTTCAACTTGCGCCAGCATCGCCACCAGt                                                                                               >  2:237978/1‑140 (MQ=255)                                   cGTGCATCTCCTGACACAACTGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGt                                                                                               >  1:514247/1‑140 (MQ=255)                                     tGCATCTCCTGACACAACTGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTtg                                                                                             >  1:187536/1‑140 (MQ=255)                                     tGCATCTCCTGACACAACTGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTtg                                                                                             >  2:849301/1‑140 (MQ=255)                                     tGCATCTCCTGACACAACTGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTtg                                                                                             >  2:555606/1‑140 (MQ=255)                                     tGCATCTCCTGACACAACTGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTtg                                                                                             <  1:849301/140‑1 (MQ=255)                                     tGCATCTCCTGACACAACTGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTtg                                                                                             >  1:491129/1‑140 (MQ=255)                                           tcCTGACACAACTGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCGGTTCAAACTGCCCCGGCATCGACACCAGTtgctgtct                                                                                       >  1:416670/1‑134 (MQ=255)                                            ccTGACACAACTGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGc                                                                                      >  2:639157/1‑140 (MQ=255)                                                      cTGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGc                                                                            >  2:5578/1‑140 (MQ=255)                                                      cTGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGc                                                                            >  1:667458/1‑140 (MQ=255)                                                       tGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCt                                                                           >  1:737616/1‑140 (MQ=255)                                                              gggTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTccgcc                                                                    <  1:639157/140‑1 (MQ=255)                                                                       cgcgATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTtaat                                                           <  1:555606/140‑1 (MQ=255)                                                                         cgATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTtaataa                                                         >  2:32486/1‑140 (MQ=255)                                                                         cgATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTGGTGCCAGGTTCACGGGGCTTTTCGCCGGAGTtaataa                                                         >  1:280303/1‑140 (MQ=255)                                                                         cgATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAATTGGGCCCGCTTCTCGGGGCTCTCCGCCGGAGTtaataa                                                         >  2:330035/1‑140 (MQ=255)                                                                         cgATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAATTGAGCCTGCTTCTCGGGGCTTTCCGCCGGAGTtaataa                                                         >  2:326630/1‑140 (MQ=255)                                                                            tAGCGCACCGGATACTTCTTATAAACGCTCGTCAACACCTCAACCAGCTGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATaaaaa                                                      <  2:280303/140‑1 (MQ=255)                                                                              gcgcACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATAAAAACa                                                    <  1:49616/140‑1 (MQ=255)                                                                                gcACCGGATACTTGTGATAAACGCTCGGCGACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATAAAAACAGa                                                  <  1:326630/140‑1 (MQ=255)                                                                                  aCCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATAAAAACAGAAt                                                <  2:606891/140‑1 (MQ=255)                                                                                            tGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATAAAAACAGAATGGAGTTgaga                                      <  1:121969/140‑1 (MQ=255)                                                                                              ttATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATAAAAACAGAATGGAGTTgaga                                      >  1:753604/1‑138 (MQ=255)                                                                                              ttATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATAAAAACAGAATGGAGTTgaga                                      <  2:753604/138‑1 (MQ=255)                                                                                                   aaCGCTCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATAAAAACAGAATGGAGTTGAGATCGCACt                               >  1:70133/1‑140 (MQ=255)                                                                                                        tCGGCAACACCTCAACCATCGGCGAGTCACCCTCAATATGCTGTTGAAATTTCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATAAAAACAGAATGGAGTTGAGATCGCACTTCTcc                          >  2:79505/1‑140 (MQ=255)                                                                                                        tCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTCTCCGCCGGAGTTTATAGAAACAGAAAGGAGTTGAGATCGCACTTCTcc                          >  2:257338/1‑140 (MQ=255)                                                                                                        tCGGCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTATCCGCCGGAGTTAATAAAAACAGAAGGGAGATGAGATCGCACTTCTcc                          >  2:230575/1‑140 (MQ=255)                                                                                                          ggCAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATAAAAACAGAATGGAGTTGAGATCGCACTTCTCCgg                        >  2:503828/1‑140 (MQ=255)                                                                                                            cAACACCTCAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATAAAAACAGAATGGAGTTGAGATCGCACTTCTCCGGca                      <  2:737616/140‑1 (MQ=255)                                                                                                                  cTCAACGAGCGGCGACTCGCCTTCAATATGCTGTTCAAATTGCGCCAGCATCGCTACCAGTTGTGCCATCTTCTCGTTGCTTTCCGCCGGAGTTAATAAAGACAGAATGGAGTGGAGATCGCACTTCTCCGGCACAAGGc                <  2:589124/140‑1 (MQ=255)                                                                                                                    cAACCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATAAAAACAGAATGGAGTTGAGATCGCACTTCTCCGGCACAATGCCg              <  2:514247/140‑1 (MQ=255)                                                                                                                      aCCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATAAAAACAGAATGGAGTTGAGATCGCACTTCTCCGGCACAATGCCGtt            <  1:503828/140‑1 (MQ=255)                                                                                                                      aCCAGCGGCGAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATAAAAACAGAATGGAGTTGAGATCGCACTTCTCCGGCACAATGCCGtt            <  2:667458/140‑1 (MQ=255)                                                                                                                        cAGCGGCGAGTCACCATCAATACGCCGTTAAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATAAAAACAGAATGGAGTTGAGATCGCACTTCTCCGGCACAATGCCGTtct          <  1:257338/140‑1 (MQ=255)                                                                                                                            ggcgAGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATAAAAACAGAATGGAGTTGAGATCGCACTTCTCCGGCACAATGCCGTTCTCACg      >  1:426568/1‑140 (MQ=255)                                                                                                                                aGTCACCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATAAAAACAGAATGGAGTTGAGATCGCACTTCTCCGGCACAATGCCGTTCTCACGCAGa  >  1:71732/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       TGTAGGTCTTTGACGTCGAAACTGACATACAGATCGTGCATCTCCTGACACAACTGGCGCAGGGTGTAGTCGCGATAGCGCACCGGATACTTGTTATAAACGCTCGGCAACACCTCAACCAGCGGCGAGTCATCCTCAATATGCTGTTCAAATTGCGCCAGCATCGCCACCAGTTGTGCCAGCTTCTCGTGGCTTTCCGCCGGAGTTAATAAAAACAGAATGGAGTTGAGATCGCACTTCTCCGGCACAATGCCGTTCTCACGCAGA  >  NC_000913/3107345‑3107611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑