Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,122,917	T→C	T297A (ACT→GCT)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,122,917	0	T	C	97.4%	132.4 / NA	39	T297A (ACT→GCT)	glcB	malate synthase G
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (19/19);  minor base A (1/0);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGA  >  NC_000913/3122781‑3123050                                                                                                                                         |                                                                                                                                      tGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGcccc                                                                                                                                    <  2:617115/140‑1 (MQ=255)  gACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCcccc                                                                                                                                   >  2:132902/1‑140 (MQ=255)  gACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCcccc                                                                                                                                   <  1:813018/140‑1 (MQ=255)        cGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATc                                                                                                                             <  1:641429/140‑1 (MQ=255)        cGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATc                                                                                                                             <  2:77543/140‑1 (MQ=255)         gTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCTTTTTTCTGCATTTTTTCTTGCAGAGCCCCCTGCATCa                                                                                                                            >  1:263775/1‑140 (MQ=255)            gCGGATAAACAGCAGCGGGCGTTCGTGCAGAAAAATTTCCGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGc                                                                                                                         <  2:307868/140‑1 (MQ=255)            gCGGATAAACAGCAGCGAGCGTACGTGCATAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGc                                                                                                                         <  1:382209/140‑1 (MQ=255)             cGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGcc                                                                                                                        >  1:215589/1‑140 (MQ=255)                  aaaCAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCcagca                                                                                                                   <  1:458040/140‑1 (MQ=255)                       gcagcGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTg                                                                                                              <  2:215589/140‑1 (MQ=255)                          gcgagcgTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCgg                                                                                                           >  1:472580/1‑140 (MQ=255)                                gTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTAcagc                                                                                                     <  1:778713/140‑1 (MQ=255)                                  ccGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTAgagcag                                                                                                   >  2:798771/1‑140 (MQ=255)                                   cGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGACCCCTGCGTCATGCTCAGCAGTTTGCGGTACAGCAgg                                                                                                  >  1:226328/1‑140 (MQ=255)                                         gagaAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTa                                                                                            <  2:675723/140‑1 (MQ=255)                                                      gCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCaa                                                                               >  1:767302/1‑140 (MQ=255)                                                           cggcggcggTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAAccgcc                                                                          <  1:699583/140‑1 (MQ=255)                                                                    tgtAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGaa                                                                 >  1:462023/1‑140 (MQ=255)                                                                              aTCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGt                                                       >  2:461089/1‑140 (MQ=255)                                                                              aTCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGt                                                       >  2:587710/1‑140 (MQ=255)                                                                              aTCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGt                                                       >  1:150150/1‑140 (MQ=255)                                                                                cGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCg                                                     >  1:679323/1‑140 (MQ=255)                                                                                    aTTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGaa                                                 <  2:150150/140‑1 (MQ=255)                                                                                     ttCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGAGTTTATCTTACGCCTCAGCCGCCGCGACCGAGTCTTCGCAGTCGAGAAt                                                >  2:224657/1‑140 (MQ=255)                                                                                      tCAGTTTACGCACGATTTGCCGCCCGTTTTTATCCGTTTACTCTTGCAAAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATg                                               <  2:263775/140‑1 (MQ=255)                                                                                        aGTTTACGCACGATTTGGCGACCGTTTTTCCCCATATTCACTTGCAGATCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTCCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGt                                             <  2:261000/140‑1 (MQ=255)                                                                                          tttACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAAACGCAGCGACCGAGTCTTGGCAGTCGAGAATGGTAc                                           >  2:250658/1‑140 (MQ=255)                                                                                            tACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGATTCTTCGCAGTCGAGAATGGTACTg                                         >  2:93931/1‑140 (MQ=255)                                                                                                    aTTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATagcagc                                 >  2:600268/1‑140 (MQ=255)                                                                                                    aTTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATagcagc                                 >  1:723955/1‑140 (MQ=255)                                                                                                             ccGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGAt                        <  1:1213/140‑1 (MQ=255)                                                                                                             ccGTTTTTCTCCATTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGAt                        >  2:819289/1‑140 (MQ=255)                                                                                                                 ttttCCCCATTTTCTCTTGCAGTGCTCCCTGCATCAGCCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACa                    <  1:224657/140‑1 (MQ=255)                                                                                                                         aTTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGAt            <  1:819289/140‑1 (MQ=255)                                                                                                                         aTTTTCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGAt            <  2:767302/140‑1 (MQ=255)                                                                                                                         aTTTTCTCTCGCAGCCCCCCCTGCGTCGGGCCCAGCAGGTTGCGGAACAGCAGGATTTTATCTTCCGCATAAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGAt            <  1:809258/140‑1 (MQ=255)                                                                                                                          ttttCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAAAGGTACTGGTAGGAGATTCGACCATAACATCGTTGAtg           >  1:274245/1‑140 (MQ=255)                                                                                                                           tttCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGAt                        <  2:198194/126‑1 (MQ=255)                                                                                                                           tttCTCTTGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGAt                        >  1:198194/1‑126 (MQ=255)                                                                                                                              ctctTGCAGAGCCCCCTACACCAGGCCCGGCAGGTGGCGGTACAGGAGGATTTTATCTTCCCCACCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTgcg       <  1:751078/140‑1 (MQ=255)                                                                                                                                  tGCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCgg   <  1:132902/140‑1 (MQ=255)                                                                                                                                   gCAGAGCCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGa  <  1:587710/140‑1 (MQ=255)                                                                                                                                   gCAGACCCCCCTGCAACAGGCCCAGAAGGCTGCGGTATAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGa  <  1:421269/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      TGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGA  >  NC_000913/3122781‑3123050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑