Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,154,600	T→C	T207A (ACT→GCT)	yqhC ←	DNA‑binding transcriptional activator YqhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,154,600	0	T	C	100.0%	91.0 / NA	27	T207A (ACT→GCT)	yqhC	DNA‑binding transcriptional activator YqhC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/11);  total (16/11)

ACAGACTTAAAATTATGGTGGAACGCCGATACGCTCATGTTGGCTTCTGCCGCCAGTTGCTCGACGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGTCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACGTAGTACAGAATTTCGCGGATGATCTGTTTGCCGAGAATACGCGCATCCAGTGGTCGCTCCATCACGTCGAGTAACCGCTCC  >  NC_000913/3154470‑3154733                                                                                                                                   |                                                                                                                                      aCAGACTTAAAATTATGGTGGAACGCCGATACGCTCATGTTGGCTTCTGCCGCCAGTTGCTCGACGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCGGCACGCGACTAGTCTGACTGAGGTGAGCCTGGCGACt                                                                                                                              >  1:239368/1‑140 (MQ=255)     aCTTAAAATTATGGTGGAACGCCGATACGCTCATGTTGGCTTCTGCCGCCAGTTGCTCGACGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGAcc                                                                                                                          <  2:115189/140‑1 (MQ=255)               aTGGTGGAACGCCGATACGCTCATGTTGGCTTCTGCCGCCAGTTGCTCGACGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCGCGCGGCTATTCACACTAAAGTGAGCCTGGCCACTGACCAGCGCCAGTa                                                                                                                >  2:284529/1‑140 (MQ=255)                  gtggAACGCCGATACGCTCATGTTGGCTTCTGCCGCCAGTTGCTCGACGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAAcg                                                                                                             <  1:473418/140‑1 (MQ=255)                     gAACGCCGATACGCTCATGTTGGCTTCTGCCGCCAGTTGCTCGACGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACgcgc                                                                                                          >  2:838013/1‑140 (MQ=255)                     gAACGCCGATACGCTCATGTTGGCTTCTGCCGCCAGTTGCTCGACGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACgcgc                                                                                                          >  1:151778/1‑140 (MQ=255)                           cGATACGCTCATGTTGGCTTCTGCCGCCAGTTGCTCGACGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGcgccgccgc                                                                                                    <  2:151778/140‑1 (MQ=255)                                       gTTGGCTTCTGCCGCCAGTTGCTCGACGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCa                                                                                        >  2:65938/1‑140 (MQ=255)                                       gTTGGCTTCTGCCGCCAGTTGCTCGACGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCa                                                                                        <  2:29692/140‑1 (MQ=255)                                       gTTGGCTTCTGCCGCCAGTTGCTCGACGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCa                                                                                        <  1:65938/140‑1 (MQ=255)                                            cTTCTGCCGCCAGTTGCTCGACGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACg                                                                                   <  2:58748/140‑1 (MQ=255)                                              tCTGCCGCCAGTTGCGCGACGCTCAGGTTGTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACgta                                                                                 <  1:284529/140‑1 (MQ=255)                                                                                 gtATTTATTCTCAATCCGTTTCGGCACGCGGCTAATCAGGCTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACGTAGTACAGAATTTCGCGGATGATCTGTTTGCCGAGaa                                              <  2:353497/140‑1 (MQ=255)                                                                                          ctcAATCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACGTAGTACAGCATTTCGCGGATCATCTGTTTGCCGAGAATACGCGCAt                                     >  1:359066/1‑140 (MQ=255)                                                                                            cAATCCGTTTCAGCACGTGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACGTAGTACAGAATTTCGCGGATGATCTGTTTGCCGAGAATACGCGCATcc                                   <  1:99874/140‑1 (MQ=255)                                                                                            cAATCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACGTAGTACAGAATTTCGCGGATGATCTGTTTGCCGAGAATACGCGCATcc                                   >  1:508377/1‑140 (MQ=255)                                                                                            cAATCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACGTAGTACAGAACTTCGCGGTTGAACTTTTTGCCTAGAATACGCGCATcc                                   >  1:354659/1‑140 (MQ=255)                                                                                             aaTCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACGTAGTACAGAATGTCGCGGAGGATGTGATTGCCGTGAATACGCGCATCCa                                  >  1:255237/1‑140 (MQ=255)                                                                                              aTCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACGTGGTACAGAAATTCGCGGGTGATCTGTGTGCCCAGAATACGCGCATCCAg                                 >  2:228685/1‑140 (MQ=255)                                                                                              aTCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACGTAGTACAGAATTTCGCGGATGATCTGTTTGCCGAGAATACGCGCATCCAg                                 >  1:216190/1‑140 (MQ=255)                                                                                              aTCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACGTAGTACAGAATTTCGCGGATGATCTGTTTGCCGAGAATACGCGCATCCAg                                 >  2:821626/1‑140 (MQ=255)                                                                                                  gTTTCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACGTAGTACAGAATTTCGCGGATGATCTGTTTGCCGAGAATACGCGCATCCAGTGGt                             >  2:520652/1‑140 (MQ=255)                                                                                                    ttCAGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACGTAGTACAGAATTTCGCGGATGATCTGTTTGCCGAGAATACGCGCATCCAGTGGTCg                           >  1:208768/1‑140 (MQ=255)                                                                                                       aGCACGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACGTAGTACAGAATTTCGCGGATGATCTGTTTGCCGAGAATACGCGCATCCAGTGGTCGCTc                        >  2:531161/1‑140 (MQ=255)                                                                                                          aCGCGGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACGTAGTACAGAATTTCGCGGATGATCTGTTTGCCGAGAATACGCGCATCCAGTGGTCGCTCCAt                     <  1:838013/140‑1 (MQ=255)                                                                                                             cgGCTAATCAGACTGAAGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACGTAGTACAGAATTTCGCGGATGATCTGTTTGCCGAGAATACGCGCATCCAGTGGTCGCTCCATCAc                  <  2:508377/140‑1 (MQ=255)                                                                                                                             aGTGAGCCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACGTAGTACAGAATTTCGCGGATGATCTGTTTGCCGAGAATACGCGCATCCAGTTGTCGCTGCATCACGTCGAGTAACCGCTcc  >  1:408353/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                      ACAGACTTAAAATTATGGTGGAACGCCGATACGCTCATGTTGGCTTCTGCCGCCAGTTGCTCGACGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGGCTAATCAGACTGAAGTGAGTCTGGCGACTGACCAGCGCCAGTAACGCGCCGCCGCAAGGTCCGGTCAGCACGTAGTACAGAATTTCGCGGATGATCTGTTTGCCGAGAATACGCGCATCCAGTGGTCGCTCCATCACGTCGAGTAACCGCTCC  >  NC_000913/3154470‑3154733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑