Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,207,655	C→T	G95G (GGC→GGT)	ttdB →	L(+)‑tartrate dehydratase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,207,655	0	C	T	100.0%	119.2 / NA	37	G95G (GGC→GGT)	ttdB	L(+)‑tartrate dehydratase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/14);  total (23/14)

AACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAG  >  NC_000913/3207524‑3207791                                                                                                                                    |                                                                                                                                         aaCGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACt                                                                                                                                  >  2:558896/1‑140 (MQ=255)         aGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTg                                                                                                                          >  1:650127/1‑140 (MQ=255)              ttttCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATGTATTGAGCAGAGCGGTGTGAATCTGGTGGTTGGCaaa                                                                                                                     >  1:230996/1‑140 (MQ=255)               tttCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAg                                                                                                                    <  2:634497/140‑1 (MQ=255)                ttCCACGCTTGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAAGCAGTATGCGTATGGAAAGTTTTGCACGTGAATCTATTGAGCAGATCGGTGTGAAACTGGTGGTTGGCAAAgg                                                                                                                   <  2:503961/140‑1 (MQ=255)                ttCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAgg                                                                                                                   <  2:650127/140‑1 (MQ=255)                ttCCACGCTGGCCCCATAGTGCGCAAAAACGCCGACAAAAGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAgg                                                                                                                   <  2:230996/140‑1 (MQ=255)                        tGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATgg                                                                                                           >  2:805097/1‑140 (MQ=255)                                 cGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGa                                                                                                  <  2:429894/140‑1 (MQ=255)                                 cGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGa                                                                                                  <  1:558896/140‑1 (MQ=255)                                    gcgcAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCg                                                                                               >  2:152268/1‑140 (MQ=255)                                    gcgcAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGAAAACCAGTATGCGTATGGACAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCAGCTGACCg                                                                                               >  2:34278/1‑140 (MQ=255)                                     cgcAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCga                                                                                              >  2:571715/1‑140 (MQ=255)                                            aCGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGc                                                                                       <  1:841835/140‑1 (MQ=255)                                                              tGGTCCCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGGGGTTGGCAAAGGTGGTATGGTGCCGCTGACCGGAGGAGGCTGTCAGAAATTCAAGgcg                                                                     >  1:417760/1‑140 (MQ=255)                                                                gTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCt                                                                   >  2:842992/1‑140 (MQ=255)                                                                gTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCt                                                                   >  1:88417/1‑140 (MQ=255)                                                                 tctcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTa                                                                  >  1:592589/1‑140 (MQ=255)                                                                 tctcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTa                                                                  >  2:518962/1‑140 (MQ=255)                                                                  ctcCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTAc                                                                 >  2:105667/1‑140 (MQ=255)                                                                       tCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAtgtg                                                            <  1:518962/140‑1 (MQ=255)                                                                       tCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAtgtg                                                            <  2:592589/140‑1 (MQ=255)                                                                       tCGGCCCGACAACCACTATGCGCATCGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAtgtg                                                            <  1:268637/140‑1 (MQ=255)                                                                          gCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGAtt                                                         >  1:715438/1‑140 (MQ=255)                                                                           cccGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGGCGCTGACCCAAGCAGGATGCCAGAACTTCAAGGCGCTACATGTGAttt                                                        >  2:342706/1‑140 (MQ=255)                                                                                     aGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAgg                                              <  1:434048/140‑1 (MQ=255)                                                                                         tGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAAATCAGGGCGCTACCTGTGCTTTTCCCGGCAGGCTgc                                          >  2:323423/1‑140 (MQ=255)                                                                                         tGCGTATGGAAAGTTTTGAACGCGACTTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTgc                                          <  1:323423/140‑1 (MQ=255)                                                                                          gCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTgcg                                         <  1:842992/140‑1 (MQ=255)                                                                                             tATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCgg                                      >  2:581605/1‑140 (MQ=255)                                                                                              aTGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGt                                     >  1:107469/1‑140 (MQ=255)                                                                                              aTGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATGCAAGGCGGTACATGTGGTTTTGCCGGCAGGCTGCGCGGt                                     >  2:231216/1‑140 (MQ=255)                                                                                               tGGAAAGGTTTGAACGCGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTg                                    >  2:571492/1‑140 (MQ=255)                                                                                                           aaCGTGAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGAGGAATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGAGATTTTCTCGGAAGGCTGCGCCGTGCTGGCGGCAAcc                        >  1:415056/1‑140 (MQ=255)                                                                                                                gAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCGGGCAGCGCGGTGCAGGCGGCAACCCAggg                   >  1:369298/1‑139 (MQ=255)                                                                                                                gAATTTATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGt                   >  2:794911/1‑140 (MQ=255)                                                                                                                    ttATTGAGCAGACCGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACGGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTGCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGaa               <  1:34278/140‑1 (MQ=255)                                                                                                                                ccGGTGTGTAACTGGTTCTTGGTAAAGGTGGTATGTGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTgaagaa   <  2:417760/140‑1 (MQ=255)                                                                                                                                 cGGTGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGaagaag  >  2:134318/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                         AACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAG  >  NC_000913/3207524‑3207791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑