Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,230,399	C→T	G504G (GGC→GGT)	ygjK →	glycoside hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,230,399	0	C	T	100.0%	100.0 / NA	32	G504G (GGC→GGT)	ygjK	glycoside hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/19);  total (13/19)

GCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAG  >  NC_000913/3230267‑3230530                                                                                                                                     |                                                                                                                                    gCCGGAATATGGGGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACg                                                                                                                              <  2:192708/140‑1 (MQ=255) gCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACg                                                                                                                              <  2:803018/140‑1 (MQ=255)  ccGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGa                                                                                                                             <  1:720323/140‑1 (MQ=255)  ccGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGa                                                                                                                             <  1:696913/140‑1 (MQ=255)  ccGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGa                                                                                                                             <  1:674889/140‑1 (MQ=255)               cgACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAAtt                                                                                                                >  1:78514/1‑140 (MQ=255)               cgACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAAtt                                                                                                                >  2:779041/1‑140 (MQ=255)               cgACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTCTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAAtt                                                                                                                <  2:593327/140‑1 (MQ=255)                gACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTc                                                                                                               >  1:191874/1‑140 (MQ=255)                       gACAAAGCCCACAACTCTGAGACCGTCGAAATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGcaca                                                                                                        <  1:361451/140‑1 (MQ=255)                       gACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGcaca                                                                                                        <  1:103122/140‑1 (MQ=255)                                cacaACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGCACAGGTtgctgc                                                                                               <  2:78514/140‑1 (MQ=255)                                            agCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATc                                                                                   <  2:158465/140‑1 (MQ=255)                                            agCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATc                                                                                   >  2:798421/1‑140 (MQ=255)                                             gcggcgAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCg                                                                                  <  1:523616/140‑1 (MQ=255)                                                               cGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATgccgcc                                                                <  2:509285/140‑1 (MQ=255)                                                                ggTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTACGGCACAGATTGATGCCACGTGGGAGTCGGGACGTGATGATGccgccg                                                               >  2:404012/1‑140 (MQ=255)                                                                    aaaaaaGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGAACAGGTTGATGCGTCGTGGGAATCGGGGCGCGATGATGCCGCCGTCtt                                                           >  2:270362/1‑140 (MQ=255)                                                                                       agaCGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGaa                                        <  2:158431/140‑1 (MQ=255)                                                                                         aCGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACa                                      >  2:478847/1‑140 (MQ=255)                                                                                          cGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAg                                     <  1:478847/140‑1 (MQ=255)                                                                                          cGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAg                                     <  1:779041/140‑1 (MQ=255)                                                                                             aGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAGTTg                                  <  1:798421/140‑1 (MQ=255)                                                                                              gTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAGTTgg                                 >  1:748442/1‑140 (MQ=255)                                                                                              gTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCATGGGAATCGGGGCGTGATGAAGCCGCCGTCTTAGGATTTATCAACAAAGAACAGTTgg                                 >  1:258893/1‑140 (MQ=255)                                                                                                 tGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAGTTGGATa                              <  2:748442/140‑1 (MQ=255)                                                                                                               aCGCCCGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACCAAGCACTGTTGGATAAATATGTCGCTAAt                >  1:292950/1‑140 (MQ=255)                                                                                                                  cccGCGTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAGTTGGATAAATATGTCGCTAATggc             >  1:3168/1‑140 (MQ=255)                                                                                                                     gcgTGGTGGAGAAAGGTCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAGTTGGATAAATATGTCGCTAATggcggc          >  1:32961/1‑140 (MQ=255)                                                                                                                      cgTGGTGGAGAAAGGTCAGTACAACCGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCa         <  1:270362/140‑1 (MQ=255)                                                                                                                           tggAGAAAGGTCAGTCCGACAGGCTGGAATTTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGt    <  1:423018/140‑1 (MQ=255)                                                                                                                             gagaAAGGTCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAg  >  2:430426/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                    GCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAG  >  NC_000913/3230267‑3230530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑