Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,266,958 C→T P36P (CCG→CCA)  tdcA ← DNA‑binding transcriptional activator TdcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,266,9580CT97.9% 151.3 / NA 48P36P (CCG→CCA) tdcADNA‑binding transcriptional activator TdcA
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (31/16);  total (32/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGA  >  NC_000913/3266826‑3267092
                                                                                                                                    |                                                                                                                                      
ttcaCGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAg                                                                                                                                 <  1:184090/140‑1 (MQ=255)
          aTGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAAc                                                                                                                            <  1:200071/135‑1 (MQ=255)
          aTGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAAc                                                                                                                            >  2:200071/1‑135 (MQ=255)
          aTGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGGTAATGATGTTAGTGACGGCTGGTTGAGTTAACCCTaa                                                                                                                       >  1:241956/1‑140 (MQ=255)
                tCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCttt                                                                                                                 >  2:756842/1‑140 (MQ=255)
                 cGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCtttt                                                                                                                >  1:704715/1‑140 (MQ=255)
                         gagaGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGTTTTTACTGACGGCTGGTTGAGTTACCCCTATTTTTTTTGCagccga                                                                                                        <  2:824891/140‑1 (MQ=255)
                         gagaGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCagccga                                                                                                        <  2:517545/140‑1 (MQ=255)
                          agagTAACAATTGACCGGCAGGTGTCAATCTTACACGCGTATTTTTCCGCCCCACTAATTCCACCCCAAAATAATCTTCAATATCTTTAATGATTTTACTGACGGCTGGTTGAGTTACCCCTAATTCTTTTGCagccgag                                                                                                       <  1:227913/140‑1 (MQ=255)
                          agagTAAAATTTGCCCGGCAGGTTTTATTTTCACCCCGTTTTTTTTCCGCACCACTAATTCCACCCAAAAAAATTCTCCATTTTCGTTAATATTTTTAGGTTCGGCTGTTTGATTTAACCCAAATTCTTTTGCacccgag                                                                                                       <  2:127528/140‑1 (MQ=255)
                                   aTTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGaa                                                                                              >  1:23739/1‑140 (MQ=255)
                                      gACCGGCAGGTGTTAATTTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTAACCCCTAATTCTTTTGCAGCCGAGCCGATAGAACCa                                                                                           <  2:655941/140‑1 (MQ=255)
                                      gACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCa                                                                                           >  2:48554/1‑140 (MQ=255)
                                       cccGGCAGGTGTTAATTCTACATCGGTATTTCTCCCCACCACTAATTCTACACAAAAATAATCTTAAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTACCCCTAATTCTTTTGCAGCCGAGCCGATAGAACCAc                                                                                          <  2:241956/139‑1 (MQ=255)
                                              ggTGTTAATTTTCCCCCGTTTTTTTCCCCCCCCCTTAATTCCCCACAAAAATAATCTTCAATTTCTTTAAGGATTTTCCGGAGGGCTGGTTGAGTTACCCCTAATTTTTTTGCGGCCGAGCCGATAGAACCACTTCTAAt                                                                                   <  2:50344/140‑1 (MQ=255)
                                              ggTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTACCCCTAATTCTTTTGCACCCGAGCCGATAGAACCACTTCTAAt                                                                                   <  2:5283/140‑1 (MQ=255)
                                                      tGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCt                                                                           >  2:454039/1‑140 (MQ=255)
                                                          acacCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGaaa                                                                       >  1:567393/1‑140 (MQ=255)
                                                                gTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTAc                                                                 >  2:838859/1‑140 (MQ=255)
                                                                gTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTAc                                                                 >  2:727015/1‑140 (MQ=255)
                                                                gTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTAc                                                                 >  2:77608/1‑140 (MQ=255)
                                                                gTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAG‑CGATAGAACCACTTCTAATGACTTCCTGAAAGACTAcc                                                                >  2:603715/1‑140 (MQ=255)
                                                                gTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTAc                                                                 >  2:444698/1‑140 (MQ=255)
                                                                 tATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTAcc                                                                >  2:574074/1‑140 (MQ=255)
                                                                    ttttCCGCACCACTAATTCCACACAAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAgg                                                             <  2:567393/140‑1 (MQ=255)
                                                                                  aaTTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCgg                                               <  1:756842/140‑1 (MQ=255)
                                                                                  aaTTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCgg                                               <  1:77608/140‑1 (MQ=255)
                                                                                  aaTTCCACACAAAAATATTCTTAAATATCGTCAATTTTTGTACTGAGGTCTGTTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCgg                                               <  2:346537/140‑1 (MQ=255)
                                                                                  aaTCCCACACAAAAAAAATCTTGAATATCGTGAATGATTCTCGTGGCGTGGGGTTGCGTAACCCATACTTTTATCGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCgg                                               <  2:334631/140‑1 (MQ=255)
                                                                                        acacCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGgaagaag                                         >  1:840136/1‑140 (MQ=255)
                                                                                        acacCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGgaagaag                                         >  1:454784/1‑140 (MQ=255)
                                                                                        acacCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCATGAAAGACTACCAGGTGCTGCGTTTTCGgaagaag                                         >  2:316066/1‑140 (MQ=255)
                                                                                        acacCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTACCTGAAAGACTCCCGGGTGCTGCGTTTTCGgaagaag                                         >  1:346537/1‑140 (MQ=255)
                                                                                          acCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGgaagaagaa                                       >  1:485657/1‑140 (MQ=255)
                                                                                          acCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGgaagaagaa                                       >  1:95773/1‑140 (MQ=255)
                                                                                          acCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGAATTCCTGAAAGACTACCAGGTGCTGCGTTTTCGgaagaagaa                                       >  2:807249/1‑140 (MQ=255)
                                                                                           ccAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAAt                                      >  1:512518/1‑140 (MQ=255)
                                                                                            aaaaaTAATCTTAAATATCGTTAATGTTTTTACTGACGGCTGGTTGATTTACCCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATa                                     <  2:749750/139‑1 (MQ=255)
                                                                                              aaaTAATCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGt                                   >  1:749750/1‑140 (MQ=255)
                                                                                               aatattCTTCAATATCGTTAATGTTTTTACTGACGGCTGGTTGAGTTACCCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTg                                  <  2:485657/140‑1 (MQ=255)
                                                                                                    tCTTCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCAt                             >  2:657478/1‑140 (MQ=255)
                                                                                                      ttCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTATTTTTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATaa                           <  1:117939/140‑1 (MQ=255)
                                                                                                      ttCAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATaa                           >  1:432733/1‑140 (MQ=255)
                                                                                                        cAATATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGCAAGACTACCAGGTGATGCGTTTTCGAAAGAAGAATAGTGCTCATAACg                         >  2:394841/1‑140 (MQ=255)
                                                                                                         aaTATCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTGTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACgc                        >  2:39484/1‑139 (MQ=255)
                                                                                                          atatCGTTAATTATTTTACTGACGGCTGGTTGAGTTACCCATAATTTTTTTGCACCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGAc                       <  2:840136/140‑1 (MQ=255)
                                                                                                          atatCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGAc                       <  1:838859/140‑1 (MQ=255)
                                                                                                          atatCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGAc                       <  1:48554/140‑1 (MQ=255)
                                                                                                          atatCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGAc                       <  1:454039/140‑1 (MQ=255)
                                                                                                          atatCGTTAATGATTTTACTGACGGCTGGGTGAGTTACCCCTAATTTTTTTGCGGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGAc                       <  1:394841/140‑1 (MQ=255)
                                                                                                           tatCGTTAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGAcc                      >  2:28544/1‑140 (MQ=255)
                                                                                                            atCGTTAATGATTTTACTGACGGCTGGTTGAGTTACCCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCt                     <  1:721686/140‑1 (MQ=255)
                                                                                                                ttAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTa                    <  2:38684/137‑1 (MQ=255)
                                                                                                                ttAATGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTa                    >  1:38684/1‑137 (MQ=255)
                                                                                                                  aaTGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGAc                       >  2:646330/1‑132 (MQ=255)
                                                                                                                  aaTGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGAc                       <  1:646330/132‑1 (MQ=255)
                                                                                                                  aaTGATTTTACTGACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTa               >  1:101478/1‑140 (MQ=255)
                                                                                                                              gcgggCTGGTTGAGTTAACCCTAATTCTTTGCCACCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTg   <  2:95773/137‑1 (MQ=255)
                                                                                                                              gACGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTg   <  2:101478/140‑1 (MQ=255)
                                                                                                                               ccGGCTGGTTGAGTTACCCCTAATTTTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGa  <  2:432733/139‑1 (MQ=255)
                                                                                                                               aCGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGa  >  2:524227/1‑140 (MQ=255)
                                                                                                                               aCGGCTGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGa  <  2:454784/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                      
TTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGA  >  NC_000913/3266826‑3267092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑