Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,327,834	A→G	A15A (GCA→GCG)	yhbY →	ribosome assembly factor YhbY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,327,834	0	A	G	100.0%	231.0 / NA	66	A15A (GCA→GCG)	yhbY	ribosome assembly factor YhbY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/30);  total (36/30)

CAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACC  >  NC_000913/3327697‑3327969                                                                                                                                          |                                                                                                                                        cAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGGATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGcgca                                                                                                                                       >  2:750723/1‑140 (MQ=255)  aaTTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCAt                                                                                                                                      <  2:584382/140‑1 (MQ=255)    ttGCGTAAATTTTTACGCGCCTATTTGGGGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTGCTAAACAGAAATAGTACCTGAAAGGTCTGGCGCATcc                                                                                                                                    >  1:328968/1‑140 (MQ=255)     tGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCg                                                                                                                                   >  2:861604/1‑140 (MQ=255)       cGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAGTCTGAGTATTAACCAATAACAGCACTTGAATGGTCTGGCGCATCCGCt                                                                                                                                 >  2:341719/1‑140 (MQ=255)       cGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCt                                                                                                                                 <  1:569998/140‑1 (MQ=255)           aaTTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAGAACAGCACCTGAGAGGTATGGCGCATCCGCTCAAg                                                                                                                             >  2:225539/1‑140 (MQ=255)              ttttACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCaa                                                                                                                              >  1:611978/1‑136 (MQ=255)              ttttACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCaa                                                                                                                              <  2:611978/136‑1 (MQ=255)                    gcgcCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAgttgtt                                                                                                                    <  1:814010/140‑1 (MQ=255)                      gcCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCt                                                                                                                  >  2:504581/1‑140 (MQ=255)                       ccTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAACGGTCTGGCGCCTCCGGCCAAGCCAGTTGTTCTg                                                                                                                 >  2:378554/1‑140 (MQ=255)                            ttGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTgg                                                                                                            <  1:823963/140‑1 (MQ=255)                                tGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCAACTGAAAGGTCTGGCGCCCCCGCTCAGGCCAGCTGTTCTGCTTGGCAGt                                                                                                        >  2:402153/1‑140 (MQ=255)                                  atatatGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCTAGTCAGTTGCTCTGCTTGGCAGTaa                                                                                                      >  1:409752/1‑140 (MQ=255)                                  atatatGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTaa                                                                                                      <  2:27046/140‑1 (MQ=255)                                     tatGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATgg                                                                                                   >  1:468892/1‑140 (MQ=255)                                     tatGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATgg                                                                                                   >  2:795599/1‑140 (MQ=255)                                     tatGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATgg                                                                                                   >  2:187106/1‑140 (MQ=255)                                           agaTGGCGGTAGAATCGCCCGTCTTCAATCCCAACGTCAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGAc                                                                                             <  1:378554/140‑1 (MQ=255)                                             aTGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCg                                                                                           >  2:778226/1‑140 (MQ=255)                                              tGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGa                                                                                          >  2:95608/1‑140 (MQ=255)                                              tGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGa                                                                                          >  1:22727/1‑140 (MQ=255)                                               ggCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCTCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGaa                                                                                         >  1:812196/1‑140 (MQ=255)                                               ggCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGc                                                                                                           <  1:806492/122‑1 (MQ=255)                                               ggCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGc                                                                                                           >  2:806492/1‑122 (MQ=255)                                               ggCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGaa                                                                                         >  1:93586/1‑140 (MQ=255)                                               ggCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGaa                                                                                         >  2:117637/1‑140 (MQ=255)                                                 cGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAgg                                                                                       <  2:733264/140‑1 (MQ=255)                                                     aGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTg                                                                                   <  2:790470/140‑1 (MQ=255)                                                          gACCCGTTTTCAATCCGAACGTTAGCAAAATATTCAATGAATCTGAGTTCTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGc                                                                              <  2:409752/140‑1 (MQ=255)                                                                     aaTCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAAc                                                                   <  1:187106/140‑1 (MQ=255)                                                                      aTCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACa                                                                  <  1:569960/140‑1 (MQ=255)                                                                      aTCCCAACGTAAGCAAAAAATAAGAGGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACa                                                                  <  1:341719/140‑1 (MQ=255)                                                                        cccAACGTAAGCAAACAATACGATGCATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAg                                                                <  1:402153/140‑1 (MQ=255)                                                                           aaCGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGt                                                             <  1:861604/140‑1 (MQ=255)                                                                           aaCGTAAGCAAAAAATAAGATGCATCTGAGTTCTATACATAAACAGCATCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGt                                                             <  2:328968/140‑1 (MQ=255)                                                                            aCGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAg                                                                >  2:545663/1‑136 (MQ=255)                                                                            aCGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAg                                                                <  1:545663/136‑1 (MQ=255)                                                                               tAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTaga                                                         <  1:504581/140‑1 (MQ=255)                                                                                 aGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCTCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGc                                                       <  2:812196/140‑1 (MQ=255)                                                                                 aGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTaga                                                         >  1:654670/1‑138 (MQ=255)                                                                                 aGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTaga                                                         <  2:654670/138‑1 (MQ=255)                                                                                 aGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGc                                                       >  2:454625/1‑140 (MQ=255)                                                                                    aaaaaaTACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCAcc                                                    <  2:93586/140‑1 (MQ=255)                                                                                       aaaTACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATg                                                 >  2:142830/1‑140 (MQ=255)                                                                                        aaTACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGa                                                <  1:95608/140‑1 (MQ=255)                                                                                             gATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACtca                                           <  2:668506/140‑1 (MQ=255)                                                                                                  aTCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAg                                      >  2:29017/1‑140 (MQ=255)                                                                                                            tAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACATGCGTTAGAGCATCACGAACTCCTCAAGGTGAAAATCg                            >  1:295389/1‑140 (MQ=255)                                                                                                            tAAACAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCg                            >  1:83108/1‑140 (MQ=255)                                                                                                              aaCAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGcc                          >  2:689288/1‑140 (MQ=255)                                                                                                                cAAAAACAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCAc                        >  1:701459/1‑140 (MQ=255)                                                                                                                   aaaCAGCACCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCTGAGATTGGACAAGCGTGAGAGCACGATGAACTCGTCAAAGTGAAAATCGCCACCGa                     >  2:247906/1‑140 (MQ=255)                                                                                                                          aCCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCg                            <  1:672350/126‑1 (MQ=255)                                                                                                                          aCCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCg                            >  2:672350/1‑126 (MQ=255)                                                                                                                          aCCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATcgc              >  2:86208/1‑140 (MQ=255)                                                                                                                          aCCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATcgc              >  1:215334/1‑140 (MQ=255)                                                                                                                          aCCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATcgc              >  1:55683/1‑140 (MQ=255)                                                                                                                          aCCTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATcgc              >  1:612146/1‑140 (MQ=255)                                                                                                                           ccTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATcgcg             <  1:535136/140‑1 (MQ=255)                                                                                                                           ccTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATcgcg             <  1:454625/140‑1 (MQ=255)                                                                                                                           ccTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATcgcg             <  1:750723/140‑1 (MQ=255)                                                                                                                            cTGAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGa            <  2:22727/140‑1 (MQ=255)                                                                                                                              gAAAGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGaaa          >  1:810163/1‑140 (MQ=255)                                                                                                                               aaaGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAAc         >  2:454261/1‑140 (MQ=255)                                                                                                                                aaGGTCTGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACt        <  1:86208/140‑1 (MQ=255)                                                                                                                                      tGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAATTCGCCATCGAAGAACGCGAAACTAAAAcc  >  1:415113/1‑140 (MQ=255)                                                                                                                                      tGGCGCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCAGGAACTCATAAAGCTGAAAATCGCCAACGAACATCGCGAAACTAAAAcc  >  2:292007/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGATCGCGAAACTAAAACC  >  NC_000913/3327697‑3327969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑