Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,329,249 T→C V96A (GTA→GCA)  dacB → peptidoglycan DD‑endopeptidase DacB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,329,2490TC98.0% 175.2 / NA 51V96A (GTA→GCA) dacBpeptidoglycan DD‑endopeptidase DacB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (27/23);  minor base G (1/0);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACAGTCAGCAGATGGCGCTGCCTGCCAGTACCCAGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGTACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAATCTGGCGTCAACCAAATCGATGGCAATGTGTTGATAGATACCTCCAT  >  NC_000913/3329119‑3329384
                                                                                                                                  |                                                                                                                                       
cacaGTCAGCAGATGGCGCTGCCTGCCAGTACCCAGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAggg                                                                                                                                <  2:595760/140‑1 (MQ=255)
cacaGTCAGCAGATGGCGCTGCCTGCCAGTACCCAGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAggg                                                                                                                                <  2:478665/140‑1 (MQ=255)
  caGTCAGCAGATGGCGCTGCCTGCCAGTACCCGGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTg                                                                                                                              >  2:595795/1‑140 (MQ=255)
  caGTCAGCAGATGGCGCTGCCTGCCAGTACCCAGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTg                                                                                                                              >  1:603768/1‑140 (MQ=255)
       agcagATGGCGCTGCCTGCCAGTACCCAGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTa                                                                                                                         >  2:92108/1‑140 (MQ=255)
       agcagATGGCGCTGCCTGCCAGTACCCAGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTa                                                                                                                         >  1:865281/1‑140 (MQ=255)
         cagATGGCGCTGCCTGCCAGTACCCAGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCCTGAAACCAGAGGAAATCTGGAAAACAGCGCGCTTAAGGGTGACTTAGt                                                                                                                       >  2:302951/1‑140 (MQ=255)
          ggATGGCGCTGCCTGACAGTACCCAGAAAATGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTg                                                                                                                      <  1:363830/139‑1 (MQ=255)
             tGGCGCTGCCTGCCAGTACCCAGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGgcg                                                                                                                   <  1:92108/140‑1 (MQ=255)
                 gcTGCCTGCCAGTACCCAGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGAt                                                                                                               >  2:705631/1‑140 (MQ=255)
                      ctgccAGTACCCAGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACCGCGGACTTAAGGGTGACCTAGTGGCGCGATTTggc                                                                                                          >  2:363830/1‑139 (MQ=255)
                                      aGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTaa                                                                                          <  2:129738/140‑1 (MQ=255)
                                       gTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATc                                                                                                   >  1:782647/1‑130 (MQ=255)
                                       gTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATc                                                                                                   <  2:782647/130‑1 (MQ=255)
                                       gTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTaaa                                                                                         <  1:16526/140‑1 (MQ=255)
                                         gATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAAc                                                                                       >  1:140797/1‑140 (MQ=255)
                                            tACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTc                                                                                    <  1:705631/140‑1 (MQ=255)
                                              cTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGAc                                                                                               <  2:866295/127‑1 (MQ=255)
                                              cTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGAc                                                                                               >  1:866295/1‑127 (MQ=255)
                                                gcgcTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGa                                                                                >  1:555798/1‑140 (MQ=255)
                                                 cgcTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGat                                                                               >  1:842264/1‑140 (MQ=255)
                                                   cTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTa                                                                                           >  1:141327/1‑126 (MQ=255)
                                                   cTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTa                                                                                           <  2:141327/126‑1 (MQ=255)
                                                   cTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGatat                                                                             <  2:824277/140‑1 (MQ=255)
                                                   cTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGCGCCTATGCGACGTTAAAACGTCAGGatat                                                                             >  1:336714/1‑140 (MQ=255)
                                                    tGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTTCCGTTCGGACGTTAAAACGTCAGGATAtt                                                                            >  1:267134/1‑140 (MQ=255)
                                                    tGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATAtt                                                                            <  2:865281/140‑1 (MQ=255)
                                                    tGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGACCCGACCTTAAAACGTCAGGATAtt                                                                            <  2:632212/140‑1 (MQ=255)
                                                      gcggcgTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCg                                                                          >  1:435606/1‑140 (MQ=255)
                                                           acagATTCAACTCGGGCCCGATTTTCGTTTTACCACGTCGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAata                                                                     <  1:294346/137‑1 (MQ=255)
                                                                         gCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCGGATCCGACATTATAACGTCAGGATAATCGCGATATGGTCGCGACTTTg                                                       >  2:241979/1‑140 (MQ=255)
                                                                         gCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAGAACATCAGGATATTCGCTATATGGTCGCGACTTTg                                                       >  2:291841/1‑140 (MQ=255)
                                                                          ccccGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGa                                                      >  1:667031/1‑140 (MQ=255)
                                                                          ccccGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGCTAACACGGCAGGATATCCGCAATATGGTCGCGACTCTgt                                                      >  2:294346/1‑139 (MQ=255)
                                                                                ttttCGTTTTACCACAACGCTTTAAACCACAGGCAATATGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAAt                                                <  1:291841/140‑1 (MQ=255)
                                                                                     gTTTTACCACGACGCTTTAAACCAAAGGCAAGGTGCAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAATCTGGc                                           <  2:267134/140‑1 (MQ=255)
                                                                                         tACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAATCTGGCGTCa                                       >  2:812121/1‑140 (MQ=255)
                                                                                                acgCTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAATCTGGCGTCAACCAAAt                                <  1:812121/140‑1 (MQ=255)
                                                                                                   cTTGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAATCTGGCGTCAACCAAATCGa                             <  1:569772/140‑1 (MQ=255)
                                                                                                    ttGAAACCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGCCGCGACTATGATAAGATCTGGAGTCAACCAAATCGAt                            >  1:411214/1‑140 (MQ=255)
                                                                                                       aaaCCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAATCTGGCGTCAACCAAATCGATGGc                         <  2:555798/140‑1 (MQ=255)
                                                                                                        aaCCAAAGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAATCTGGCGTCAACCAAATCGATGGCa                        <  2:842264/140‑1 (MQ=255)
                                                                                                         aCCAAAGGTAATGTGGTAAACGTCGCACTTTAGTGCGACGTAGTGGCGAGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAATCTGGCGTCAACCAAATCGATGGCaa                       <  2:411214/140‑1 (MQ=255)
                                                                                                         aCCAAAGGCAATGTGTAAAACGACGCACTCAAGGGTGGCTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAATCTGGCGTCAACCAAATCGATGGCaa                       <  1:241979/140‑1 (MQ=255)
                                                                                                             aaGGCAATGTGGAAAACGGCGCCCTTCAGGGTGAGTTAGTGGCGCGCTTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCCAGTTTGAAAAAATCTGGCCACAACCAAATCGATGGCAAtgtg                   <  2:777704/140‑1 (MQ=255)
                                                                                                             aaGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTAGAAAAAAGCTGTCGTCAACCATATCGATGGCAAtgtg                   >  1:257883/1‑140 (MQ=255)
                                                                                                              aGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAATCTGGCGTCAACCAAATCGATGGCAATgtgt                  >  2:148827/1‑140 (MQ=255)
                                                                                                              aGGCAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAATCTGGCGTCAACCAAATCGATGGCAATgtgt                  >  2:27636/1‑140 (MQ=255)
                                                                                                                 cAATGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAATCTGGCGTCAACCAAATCGATGGCAATGTGTTga               <  1:595795/140‑1 (MQ=255)
                                                                                                                  aaTGTGGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAATCTGGCGTCAACCAAATCGATGGCAATGTGTTgat              >  1:130487/1‑140 (MQ=255)
                                                                                                                    tgtgGAAAACGGCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAATCTGGCGTCAACCAAATCGATGGCAATGTGTTgatag            >  1:683476/1‑140 (MQ=255)
                                                                                                                              ggCGCACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAATCTGGCGTCAACCAAATCGATGGCAATGTGTTGATAGATACCTCCAt  >  1:859826/1‑140 (MQ=255)
                                                                                                                                  |                                                                                                                                       
CACAGTCAGCAGATGGCGCTGCCTGCCAGTACCCAGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGTACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAATCTGGCGTCAACCAAATCGATGGCAATGTGTTGATAGATACCTCCAT  >  NC_000913/3329119‑3329384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑