Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,354,811	G→A	V29V (GTG→GTA)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,354,811	0	G	A	100.0%	79.0 / NA	24	V29V (GTG→GTA)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/9);  total (15/9)

CAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTGCTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGC  >  NC_000913/3354676‑3354946                                                                                                                                        |                                                                                                                                        cAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTACGTa                                                                                                                                     <  1:528602/140‑1 (MQ=255)               tGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACTATATGTTGTACGATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCa                                                                                                                       <  2:592293/140‑1 (MQ=255)                    ttCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCACTGGc                                                                                                                  <  2:26592/140‑1 (MQ=255)                                                   tgttgtACGATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGcc                                                                                   <  1:735705/140‑1 (MQ=255)                                                    gttgtACGATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCg                                                                                  >  2:576066/1‑140 (MQ=255)                                                           gATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTaa                                                                           >  2:770613/1‑140 (MQ=255)                                                           gATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTaa                                                                           >  1:425014/1‑140 (MQ=255)                                                               aaTCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCGCTGGCCCGGATGGAGCGCCGTGGCGAGATTGTCGCCGATGGTAAAAcc                                                                       >  2:218855/1‑140 (MQ=255)                                                                   ccTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCg                                                                   <  1:770613/140‑1 (MQ=255)                                                                    cTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCACTGGTCCGCATGCCGCAACATAGCGCGATTTTCGCAGATGGTAATACCGGCGa                                                                  >  2:403578/1‑140 (MQ=255)                                                                     ttGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGAc                                                                 >  2:2771/1‑140 (MQ=255)                                                                              aTAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGc                                                        >  1:847619/1‑140 (MQ=255)                                                                                       gTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTCAAAACGGCGACGGTTGCGGCTTGCTGTTa                                               >  1:286053/1‑140 (MQ=255)                                                                                       gTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTGCTGTTa                                               >  1:490464/1‑140 (MQ=255)                                                                                          tCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCGGATTGTAGAACCGGCGCCGGTTGCGGCTTGCTGTTACaa                                            >  2:330489/1‑140 (MQ=255)                                                                                             gCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATGCTCGCCGAAGGTCAAAGCGGCGACGGTTGCCGCTTGCTGTTACaaaaa                                         >  2:295712/1‑140 (MQ=255)                                                                                               cTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTGCTGTTACAAAAAcc                                       >  2:810083/1‑140 (MQ=255)                                                                                               cTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTGCTGTTACAAAAAcc                                       >  1:476868/1‑140 (MQ=255)                                                                                                                 ggCGAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGCCGACGGTTACGGATTGCTGTTACAACAACCTGATCGCTTTTTTCGCAt                     >  2:321986/1‑140 (MQ=255)                                                                                                                 ggCGAACCTAGCCACAAGGTAGCACTTACCGCAATACGCGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTGCTGTTACAAAAACCGGATCGCTTTTTTCGCAt                     <  1:321986/140‑1 (MQ=255)                                                                                                                    gAACCTAGCCACAAGGTAGTACGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCGTGCTGTTACAAGAATCGGATCGCTTTTTTCGCATCGt                  >  1:248297/1‑140 (MQ=255)                                                                                                                        cTAGCCACAAGGTAGAACGTACTGCAATACACGCACTTGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTGCTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTgcg              <  1:295712/140‑1 (MQ=255)                                                                                                                           gCCACAAGGTAGTACGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTGCTGTAACAAAAAACGGGTCACCTTTTACGCATCGTTGCGCAg           >  1:225944/1‑140 (MQ=255)                                                                                                                                aaGGTAGTACGTACTGCAATACACGCACTGGCCCGCATGCAGTGCCGTGGCGCGATTCTCGCCGATGGTAAACCCGGCGACGGTTGCGGCTTGCTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAgcg      <  1:481898/140‑1 (MQ=255)                                                                                                                                    tAGTACGTACTGCAATATACGCACTGGCCCGCATGCAGCTCCGTGGCGGGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTGCTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGc  <  2:248297/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTGCTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGC  >  NC_000913/3354676‑3354946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑