Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,360,574	T→C	G459G (GGT→GGC)	gltD →	glutamate synthase subunit GltD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,360,574	0	T	C	100.0%	162.0 / NA	47	G459G (GGT→GGC)	gltD	glutamate synthase subunit GltD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/24);  total (23/24)

CGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTT  >  NC_000913/3360439‑3360709                                                                                                                                        |                                                                                                                                        cGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTa                                                                                                                                     <  1:464720/140‑1 (MQ=255)         aTTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCa                                                                                                                             <  2:205065/140‑1 (MQ=255)          ttCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCa                                                                                                                             <  1:105505/139‑1 (MQ=255)          ttCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCa                                                                                                                             >  2:105505/1‑139 (MQ=255)          ttCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAg                                                                                                                            <  1:545951/140‑1 (MQ=255)           tCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGa                                                                                                                           <  1:764500/140‑1 (MQ=255)           tCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGa                                                                                                                           <  1:40443/140‑1 (MQ=255)             acaAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACg                                                                                                                         <  2:672134/140‑1 (MQ=255)             acaAGGCCGCATCATAGCCCCGGAACGCAGCGACAACACCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACg                                                                                                                         <  1:365760/140‑1 (MQ=255)                  gCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGtatt                                                                                                                    >  1:123079/1‑140 (MQ=255)                  gCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGAACGCTATTGCCGGAGGCCGTAAAGCGGCAGACGGtatt                                                                                                                    >  1:246289/1‑140 (MQ=255)                  gCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGAACTGGTGGTGACCGCTATTGCCGAAGCCCGTTAGGCGGCAGACGGtatt                                                                                                                    >  2:365760/1‑140 (MQ=255)                    cGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGtattat                                                                                                                  >  1:202911/1‑140 (MQ=255)                      catcatCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGa                                                                                                                >  2:156050/1‑140 (MQ=255)                       atcatcGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGa                                                                                                                           <  2:681579/128‑1 (MQ=255)                       atcatcGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGa                                                                                                                           >  1:681579/1‑128 (MQ=255)                       atcatcGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGaa                                                                                                               >  1:664835/1‑140 (MQ=255)                       atcatcGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAAGCCTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCTGTAAGGCGGCAGACGGTATTATGaa                                                                                                               <  2:586763/140‑1 (MQ=255)                             gCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAActggct                                                                                                         <  2:202911/140‑1 (MQ=255)                             gCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAActggct                                                                                                         >  1:652299/1‑140 (MQ=255)                              ccccGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAActggctg                                                                                                        <  1:26196/140‑1 (MQ=255)                                        cAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAg                                                                                              <  2:25745/140‑1 (MQ=255)                                        cAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAg                                                                                              <  2:849084/140‑1 (MQ=255)                                           cGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAActggctg                                                                                                        <  2:478351/127‑1 (MQ=255)                                           cGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAActggctg                                                                                                        >  1:478351/1‑127 (MQ=255)                                                aCGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAActggctgg                                                                                                       >  2:428487/1‑123 (MQ=255)                                                aCGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAActggctgg                                                                                                       <  1:428487/123‑1 (MQ=255)                                                     ttCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATg                                                                                 >  1:11194/1‑140 (MQ=255)                                                      tCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGa                                                                                <  1:156050/140‑1 (MQ=255)                                                               gCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAg                                                                       <  2:11194/140‑1 (MQ=255)                                                                cAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTc                                                                            <  2:134414/134‑1 (MQ=255)                                                                cAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTc                                                                            >  1:134414/1‑134 (MQ=255)                                                                cAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGa                                                                      >  2:52026/1‑140 (MQ=255)                                                                  aCCCGAAAATCTTTGCTGGCGGCGATATCGTGCGTAGTTGCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACt                                                                    <  2:307488/140‑1 (MQ=255)                                                                                   ggcggcGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCaaa                                                   >  2:69397/1‑140 (MQ=255)                                                                                      ggcgATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTa                                                <  2:652299/140‑1 (MQ=255)                                                                                              cGTCCGTGGTTCCGATCCGGTGCTGACCGCTATTGGCGACGGCCGTAACGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGaa                                        <  2:246289/140‑1 (MQ=255)                                                                                                 ccGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTg                                     <  1:52026/140‑1 (MQ=255)                                                                                                                ggtggtGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTgg                      >  1:77904/1‑140 (MQ=255)                                                                                                                 gtggtgACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGc                     >  2:622841/1‑140 (MQ=255)                                                                                                                 gtggtgACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGc                     >  1:753934/1‑140 (MQ=255)                                                                                                                       aCCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCACATTACTGCCGATGTGCGCGTTGTTTTCTGGCAAATGc               >  1:392978/1‑140 (MQ=255)                                                                                                                       aCCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGACGTACGCGTTCTTTTCTGGCAAATGc               >  1:407043/1‑140 (MQ=255)                                                                                                                       aCCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGc               >  2:450404/1‑140 (MQ=255)                                                                                                                         cGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAGATTGCTGTCGAAGTGCACGTTGTTTTCTGGCAAATGCgg             >  2:274845/1‑140 (MQ=255)                                                                                                                         cGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCgg             >  2:521740/1‑140 (MQ=255)                                                                                                                              ttGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGa        >  2:716233/1‑140 (MQ=255)                                                                                                                                    aaGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGttt  <  2:770734/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTT  >  NC_000913/3360439‑3360709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑