Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,402,255 T→C T360A (ACC→GCC)  csrD ← regulator of CsrB and CsrC decay

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,402,2550TC98.3% 202.0 / NA 59T360A (ACC→GCC) csrDregulator of CsrB and CsrC decay
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (29/29);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTCGATAAGCGTACGCCAGCGAACATTACCGCGTCCTTTTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGTATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTCCGGTGC  >  NC_000913/3402120‑3402391
                                                                                                                                       |                                                                                                                                        
gCTCGATAAGCGTACGCCAGCGAACATTACCGCGTCCTTTTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATcc                                                                                                                                      >  2:117452/1‑140 (MQ=255)
   cGATAAGCGTACGCCAGCGAACATTACCGCGTCCTTTTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGa                                                                                                                                   >  1:760803/1‑140 (MQ=255)
     aTAAGCGTACGCCAGCGAACATTACCGCGTCCTTTTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGAcc                                                                                                                                 >  1:801712/1‑140 (MQ=255)
      tAAGCGTACGCCAGCGAACATTACCGCGTCCTTTTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTTCAATACCTGATCGGCATCCTGACCa                                                                                                                                >  1:279889/1‑140 (MQ=255)
       aaGCGTACGCCAGCGAACATTACCGCGTCCTTTTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCAc                                                                                                                               <  1:859687/140‑1 (MQ=255)
            tACGCCAGCGAACATTACCGCGTCCTTTTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTAGCAGACTCGGCATGTTCCATTACCTGCTCGACATCCTGACCACTACGc                                                                                                                          >  2:340248/1‑140 (MQ=255)
              cGCCAGCGAACATTACCGCGTCCTTTTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCa                                                                                                                        >  1:493273/1‑140 (MQ=255)
               gCCAGCGAACATTACCGTGTCCCTTTTCAGGCAACCAGTAATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAg                                                                                                                       <  2:291523/140‑1 (MQ=255)
                 cAGCGAACATTACCGCGTCCTTGTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGgc                                                                                                                     <  1:340248/140‑1 (MQ=255)
                    cGAACATTACCGCGTCCTTTTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCa                                                                                                                  >  2:1107/1‑140 (MQ=255)
                    cGAACATTACCGCGTCCTTTTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCa                                                                                                                  <  1:518458/140‑1 (MQ=255)
                    cGAACATTACCGCGTCCTTTTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGAAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCa                                                                                                                  <  1:813404/140‑1 (MQ=255)
                       aCATTGCCGCGTCCTTTTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTGCGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGAt                                                                                                               <  2:827975/140‑1 (MQ=255)
                         aTTACCGCGTCCTTTTTTAGGCCACGAGTCAACGTAAATCGCCCAGCTCTTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATAc                                                                                                             <  2:375409/140‑1 (MQ=255)
                           tACCGCGTCCTTTTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCa                                                                                                           <  1:828030/140‑1 (MQ=255)
                           tACCGCGTCCTTTTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGCTCCATTACATGCGCGGTATCCTGACAACTATGCCAGGCGCAGATACCa                                                                                                           >  2:351137/1‑140 (MQ=255)
                           tACCGCGTCCTTTTTCACGCAACGAGTCATCTTAATTAGTCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCa                                                                                                           <  2:268229/140‑1 (MQ=255)
                              cgcgTCCTTTTTCAGTCAACGACTCTTCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAAtg                                                                                                        <  1:268670/140‑1 (MQ=255)
                              cgcgTCCTTTTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAAtg                                                                                                        <  2:493273/140‑1 (MQ=255)
                                cgTCCTTTTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAAtgtg                                                                                                      <  2:865429/140‑1 (MQ=255)
                                  tCCTTTTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCCTCCTGACTACTACGGCAGGCGCAGATACCAATGTGGa                                                                                                    >  1:259155/1‑140 (MQ=255)
                                      ttttCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCat                                                                                                <  1:52161/140‑1 (MQ=255)
                                      ttttCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTAGCAGACTCTGCAAGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCat                                                                                                <  2:72327/140‑1 (MQ=255)
                                                aCGAGTCATCGTAAATATCCCACCTATTGCCACCCCGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGa                                                                                      <  2:259155/140‑1 (MQ=255)
                                                    gTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGa                                                                                  <  2:801712/140‑1 (MQ=255)
                                                    acagtCGTAAATAGCGCAGCTACTGACGCCCTGCAATGCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGa                                                                                  <  1:411326/136‑1 (MQ=255)
                                                           tAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCAtttt                                                                           >  1:702122/1‑140 (MQ=255)
                                                                 gCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGtt                                                                     >  1:47102/1‑140 (MQ=255)
                                                                  cccAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTc                                                                    >  2:103188/1‑140 (MQ=255)
                                                                   ccAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGAAGATACCAATGTGGATCCTATCGTCGCGATTGAGCATTTTATTgctgt                                                                   >  2:411326/1‑136 (MQ=255)
                                                                      gCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCCCGATCGAGCATTTTATTGTTCGGCa                                                                >  1:350018/1‑140 (MQ=255)
                                                                       cTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCaa                                                               >  1:727697/1‑140 (MQ=255)
                                                                              cgccCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCa                                                        <  1:1107/140‑1 (MQ=255)
                                                                                cccTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAAc                                                      >  1:591158/1‑140 (MQ=255)
                                                                                 ccTGCAATCCCGCATTACGCGTGGCAGACTATGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCAGTTGATTGTTCGGCAAGGTATCAACg                                                     >  1:409840/1‑140 (MQ=255)
                                                                                  cTGCAATCCCGCATTACCCGTGCCAGACTCTCCATCTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACgg                                                    <  2:279889/140‑1 (MQ=255)
                                                                                             cATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCaa                                                       >  1:65974/1‑126 (MQ=255)
                                                                                             cATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCaa                                                       <  2:65974/126‑1 (MQ=255)
                                                                                             cATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACCGCTTTGATTAAc                                         >  1:137281/1‑140 (MQ=255)
                                                                                                  cgcatGGCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGAcc                                    >  2:471479/5‑140 (MQ=255)
                                                                                                       ggCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGa                               <  1:18399/140‑1 (MQ=255)
                                                                                                        gCAGACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGAt                              <  1:103188/140‑1 (MQ=255)
                                                                                                           ggctctGCATGTTCCATGACCTACTCGGCATCCTGCCCATTAGGCCAGACGCAGATACCAACGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGct                           <  2:350018/138‑1 (MQ=255)
                                                                                                           gACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGct                           <  2:702122/140‑1 (MQ=255)
                                                                                                           gACTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAAAGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGct                           <  2:137281/140‑1 (MQ=255)
                                                                                                            aCTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGctc                          >  2:594106/1‑140 (MQ=255)
                                                                                                            aCTCTGCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGctc                          >  1:77653/1‑140 (MQ=255)
                                                                                                                 gCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTGc                     <  2:727697/140‑1 (MQ=255)
                                                                                                                 gCATGTTCCATTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTGc                     <  2:760803/140‑1 (MQ=255)
                                                                                                                        ccATTACCTGCTCGGCAACCTGCCCACTACGCCGGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCtttt              <  2:409840/140‑1 (MQ=255)
                                                                                                                          aTTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTaa            >  2:860254/1‑140 (MQ=255)
                                                                                                                          aTTACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTaa            >  2:813017/1‑140 (MQ=255)
                                                                                                                            tACCTGCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTAACAACGGCTCTGAATCAGTGACCGGCAATGCAATCTGCCTCTTTTAACg          >  2:372519/1‑140 (MQ=255)
                                                                                                                               cTGCTCGGCATCCTGGCCACTAGGCCAGGTGCAGATAGCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTcc       <  1:393788/140‑1 (MQ=255)
                                                                                                                                 gCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTCCgg     >  1:791385/1‑140 (MQ=255)
                                                                                                                                 gCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTCCgg     >  1:430254/1‑140 (MQ=255)
                                                                                                                                 gCTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTCCgg     <  1:471479/140‑1 (MQ=255)
                                                                                                                                  cTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTCCGGt    >  1:704755/1‑140 (MQ=255)
                                                                                                                                  cTCGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTCCGGt    >  2:807024/1‑140 (MQ=255)
                                                                                                                                    cGGCATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAATTGAGCTGCGATGCTCTTTGCCGCTTTTAACGTCCGGTGc  >  2:393788/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                        
GCTCGATAAGCGTACGCCAGCGAACATTACCGCGTCCTTTTTCAGGCAACGAGTCATCGTAAATAGCCCAGCTATTGCCGCCCTGCAATCCCGCATTACGCGTGGCAGACTCTGCATGTTCCATTACCTGCTCGGTATCCTGACCACTACGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTCCGGTGC  >  NC_000913/3402120‑3402391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑