Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,494,433 C→T C141C (TGC→TGT)  nirB → nitrite reductase catalytic subunit NirB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,494,4330CT100.0% 159.9 / NA 48C141C (TGC→TGT) nirBnitrite reductase catalytic subunit NirB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/24);  total (24/24)

CGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGCGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAATTTGCCCCTATGCTGATGGCAGAACAGC  >  NC_000913/3494298‑3494563
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cGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGGACTATTGAAGACCTGAACGCCATTGAATCCTGTGccc                                                                                                                                >  1:261345/1‑140 (MQ=255)
    cGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCg                                                                                                                            >  2:8790/1‑140 (MQ=255)
       aCCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCgcag                                                                                                                         >  1:613958/1‑140 (MQ=255)
               ttATGACAGGCTGATCAAGGCAGCCGGTTCAGACCCGTGAATCCCGCCAATCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAAcgcg                                                                                                                 <  2:416266/140‑1 (MQ=255)
                tATGACAAGCTGATCATGGCAACCGTTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCgg                                                                                                                <  2:543622/140‑1 (MQ=255)
                 aTGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGt                                                                                                               <  1:47461/140‑1 (MQ=255)
                     cAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCg                                                                                                           >  1:467892/1‑140 (MQ=255)
                                tGGCAACCGGTTCCTACGCGTGTATCCCGCCTATCCAAGCTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTggc                                                                                                <  2:261345/140‑1 (MQ=255)
                                tGGCAACCGGTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTggc                                                                                                <  1:8790/140‑1 (MQ=255)
                                 ggCAACCGGTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTggcg                                                                                               >  1:580178/1‑140 (MQ=255)
                                  gCAACCGGTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGt                                                                                                               <  2:20962/123‑1 (MQ=255)
                                  gCAACCGGTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGt                                                                                                               >  1:20962/1‑123 (MQ=255)
                                      ccGGTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCtt                                                                                          >  2:723249/1‑139 (MQ=255)
                                      ccGGTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTg                                                                                          >  2:559534/1‑140 (MQ=255)
                                       cGGTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGt                                                                                         <  2:613958/140‑1 (MQ=255)
                                        ggTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGtt                                                                                        <  2:485756/140‑1 (MQ=255)
                                                      gATCCCGCCAATCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCg                                                                          >  1:708601/1‑140 (MQ=255)
                                                                 tCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTg                                                               >  2:110485/1‑140 (MQ=255)
                                                                          cTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTa                                                      <  1:476450/140‑1 (MQ=255)
                                                                          cTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTa                                                      <  1:110485/140‑1 (MQ=255)
                                                                               aCTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTAt                                                 <  2:688421/140‑1 (MQ=255)
                                                                                  cAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGa                                              >  1:535891/1‑140 (MQ=255)
                                                                                   aGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGaa                                             >  1:135868/1‑140 (MQ=255)
                                                                                     gACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAAc                                           <  2:135868/140‑1 (MQ=255)
                                                                                        tGCTTTGTCTATCGCACTATTGAAGACCTCTACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCa                                        >  1:1810/1‑140 (MQ=255)
                                                                                          cTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCGGCAGGCGGGCTGAAGAACTTAAGTATTGAAACCCACg                                      >  1:245949/1‑140 (MQ=255)
                                                                                          cTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACg                                      <  2:708601/140‑1 (MQ=255)
                                                                                          cTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACg                                      >  2:752807/1‑140 (MQ=255)
                                                                                                    cGCACTATTGAAGACCTCTACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAAtt                            <  2:1810/140‑1 (MQ=255)
                                                                                                    cGCACTATTGAAGACCTCAAGGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGTTATTGAAACCCACGTTATCGAAtt                            <  2:580178/140‑1 (MQ=255)
                                                                                                    cGCACTATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAAtt                            <  2:535891/140‑1 (MQ=255)
                                                                                                         tATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAATTTGccc                       <  2:91537/140‑1 (MQ=255)
                                                                                                         tATTGAAGACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAATTTGccc                       >  1:179718/1‑140 (MQ=255)
                                                                                                                gACCTCAACGCCATTGACTCCTATGCCCGTCACAGCAAATGCGGTGCCTTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAATTTGCCCCTATGCt                <  2:245949/140‑1 (MQ=255)
                                                                                                                gACCTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAATTTGCCCCTATGCt                <  1:752807/140‑1 (MQ=255)
                                                                                                                   cTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAATTTGCCCCTATGCTGAt             >  1:821235/1‑140 (MQ=255)
                                                                                                                   cTCAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAATTTGCCCCTATGCTGAt             >  1:672064/1‑140 (MQ=255)
                                                                                                                     cAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAATTTGCCCCt                     <  2:529815/130‑1 (MQ=255)
                                                                                                                     cAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAATTTGCCCCt                     >  1:529815/1‑130 (MQ=255)
                                                                                                                     cAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAATTTGCCCCTATGCt                <  2:530659/135‑1 (MQ=255)
                                                                                                                     cAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAATTTGCCCCTATGCt                <  1:76668/135‑1 (MQ=255)
                                                                                                                     cAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAATTTGCCCCTATGCt                >  1:530659/1‑135 (MQ=255)
                                                                                                                     cAACGCCATTGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAATTTGCCCCTATGCt                >  2:76668/1‑135 (MQ=255)
                                                                                                                             ttGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAATTTGCCCCTATGCTGATGGCAGAACAg   >  1:601315/1‑140 (MQ=255)
                                                                                                                             ttGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAATTTGCCCCTATGCTGATGGCAGAACAg   >  1:180524/1‑140 (MQ=255)
                                                                                                                             ttGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCATTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCGCGTTATCAAATGTGGCCCTATACTGATGGCAGAACAg   >  1:250271/1‑140 (MQ=255)
                                                                                                                              tGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAATTTGCCCCTATGCTGATGGCAGAACAGc  <  1:659795/140‑1 (MQ=255)
                                                                                                                              tGAATCCTGTGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAATTTGCCCCTATGCTGATGGCAGAACAGc  <  1:559534/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                  
CGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTGATACTCAGGACTGCTTTGTCTATCGCACTATTGAAGACCTCAACGCCATTGAATCCTGCGCCCGTCGCAGCAAACGCGGTGCCGTTGTTGGTGGCGGCCTGTTAGGTCTGGAAGCCGCAGGCGCGCTGAAAAACTTAGGTATTGAAACCCACGTTATCGAATTTGCCCCTATGCTGATGGCAGAACAGC  >  NC_000913/3494298‑3494563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑