Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,504,971	C→T	V350V (GTG→GTA)	yhfS ←	putative aminotransferase YhfS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,504,971	0	C	T	100.0%	145.0 / NA	43	V350V (GTG→GTA)	yhfS	putative aminotransferase YhfS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/19);  total (24/19)

TTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCA  >  NC_000913/3504839‑3505101                                                                                                                                     |                                                                                                                                   ttttACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGt                                                                                                                               <  2:169708/138‑1 (MQ=255) ttttACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGt                                                                                                                               >  1:169708/1‑138 (MQ=255)              aCGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCg                                                                                                                >  2:485385/1‑140 (MQ=255)                   ccccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCg                                                                                                           >  2:68049/1‑140 (MQ=255)                      cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGAt                                                                                                              >  2:49435/1‑134 (MQ=255)                      cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGAt                                                                                                              <  1:49435/134‑1 (MQ=255)                               aCCCTCTCCCCAAAGTGGCGAGTGGACCGTTCAGTACTGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGaa                                                                                               <  1:256799/140‑1 (MQ=255)                               aCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGaa                                                                                               <  1:199383/140‑1 (MQ=255)                                 ccTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATc                                                                                             <  1:68049/140‑1 (MQ=255)                                    ctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGca                                                                                          <  2:118447/140‑1 (MQ=255)                                    ctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGca                                                                                          <  1:608128/140‑1 (MQ=255)                                    ctcCCCAAAGGGGCGAGCGGACGGTTCAGTAGGGACTTTCGCTTGACGCTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGca                                                                                          <  2:231188/140‑1 (MQ=255)                                     tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac                                                                                         >  1:47966/1‑140 (MQ=255)                                     tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac                                                                                         >  2:125024/1‑140 (MQ=255)                                     tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTATCTTCACTGCTACGATTCGGGGTAATGCGAATCGcac                                                                                         >  1:231188/1‑140 (MQ=255)                                     tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCAGCAGTACCGTCTTTTCGCCGCTGCGATTCGTGTTAATGCGAATCGcac                                                                                         >  2:228319/1‑140 (MQ=255)                                     tcCCCAAAGGGGCGAAGGGACCCTTCAGTTCGGAATTGAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac                                                                                         <  1:344481/140‑1 (MQ=255)                                       cccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACaa                                                                                       <  1:485385/140‑1 (MQ=255)                                                            ttCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGcc                                                                  >  1:654404/1‑140 (MQ=255)                                                                   cGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGaa                                                           <  1:590218/140‑1 (MQ=255)                                                                                gACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAg                                              >  1:499981/1‑140 (MQ=255)                                                                                               cATTAAATACTGGCACTACTCTAACCCAACATCCGCAATACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAgcgg                               <  1:228319/140‑1 (MQ=255)                                                                                               cATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGTTTGTGGATCCGCTTGGGGAAACGTCGCGGAAAGGCGATAAAAGAgcgg                               >  2:284395/1‑140 (MQ=255)                                                                                               cATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGCTTGTGGATTCGCATGGGGAAACGTTTCGGAAAGGCGATAAAAGAgcgg                               >  2:275368/1‑140 (MQ=255)                                                                                               cATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAgcgg                               >  2:574462/1‑140 (MQ=255)                                                                                               cATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAgcgg                               >  1:152413/1‑140 (MQ=255)                                                                                                 ttAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAgcggcg                             <  1:125024/140‑1 (MQ=255)                                                                                                 ttAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAgcggcg                             >  1:474064/1‑140 (MQ=255)                                                                                                 ttAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACCGCCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGTTAAAAGAgcggcg                             >  2:13306/1‑140 (MQ=255)                                                                                                  tAAATACTGGCAATACTCTCACGCAAAATCCGCAGTACTGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGcggcgg                            >  2:702157/1‑140 (MQ=255)                                                                                                       aCTGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGAttt                       <  2:47966/140‑1 (MQ=255)                                                                                                         tGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAACCGTTCCGCAAACGCTATAAAATAGCGGCGGGATTTCa                     >  1:362316/1‑140 (MQ=255)                                                                                                         tGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAgcg                                >  2:548853/1‑129 (MQ=255)                                                                                                         tGGCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAgcg                                <  1:548853/129‑1 (MQ=255)                                                                                                          ggCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGtttttt                    >  2:859659/1‑137 (MQ=255)                                                                                                           gCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCata                   >  1:66793/1‑140 (MQ=255)                                                                                                           gCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCata                   >  2:621343/1‑140 (MQ=255)                                                                                                           gCAATACTCTCACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCata                   >  1:27481/1‑140 (MQ=255)                                                                                                                aCTCTCACGCAAAATCCTCAGTACCGTCTCTGCACGGCTGCGATTCGGTTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCg              <  2:362316/140‑1 (MQ=255)                                                                                                                  tctcACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGAc            <  2:66793/140‑1 (MQ=255)                                                                                                                   ctcACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGAct           <  1:859659/140‑1 (MQ=255)                                                                                                                    tcACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGActc          >  1:442876/1‑140 (MQ=255)                                                                                                                     cACGCAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGActct         >  1:31220/1‑140 (MQ=255)                                                                                                                         cAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTTATATGTCTActcttccc     >  2:465559/1‑136 (MQ=255)                                                                                                                         cAAAATCCGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGcac     <  2:152413/140‑1 (MQ=255)                                                                                                                            aacccGCAGTACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGAGGGATTTCATATTTCGACTCTGcacccg  <  2:654404/137‑2 (MQ=255)                                                                                                                                     |                                                                                                                                   TTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCA  >  NC_000913/3504839‑3505101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑