Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,524,518	T→C	F550L (TTC→CTC)	mrcA →	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,524,518	0	T	C	100.0%	68.6 / NA	21	F550L (TTC→CTC)	mrcA	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/9);  total (12/9)

ATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAA  >  NC_000913/3524413‑3524652                                                                                                          |                                                                                                                                       aTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCCTCCCGGCAGAACACATTGCCCACACCGAATcgct                                                                                                      >  1:329695/1‑140 (MQ=255) aTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCCTCCCGGCACAAAACATTGTCCACACCGAATcgct                                                                                                      >  1:581602/1‑140 (MQ=255)      cTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCCTCCCGGCACAAAACATTGTCCACACCGAATcgctggcgc                                                                                                 >  2:703541/1‑140 (MQ=255)                 ggCTGGGTTAGTCGCAGTACGTGGTGATGGTCCGCGCAGTGCGTCCGATGGGCGTTGCCTACGCTGCAGAATATCTGCAACGCTTCGGCCTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCg                                                                                      <  1:537260/140‑1 (MQ=255)                      ggTCAGTCGAAAAACTTGGTGACGGCACGAGCAATGCTGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCCTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCtt                                                                                 <  1:224482/140‑1 (MQ=255)                      ggTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCCTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCtt                                                                                 <  1:703541/140‑1 (MQ=255)                         cAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCCTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCAc                                                                              <  2:28539/140‑1 (MQ=255)                          aGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCCTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCAcc                                                                             >  2:746571/1‑140 (MQ=255)                                        gtgATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCCTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGgc                                                               >  2:124854/1‑140 (MQ=255)                                           aTGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCCTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGgcgcg                                                            <  1:124854/140‑1 (MQ=255)                                              gTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCCTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGGCGCGCgg                                                         >  1:459849/1‑140 (MQ=255)                                              gTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCCTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGGCGCGCgg                                                         >  2:436200/1‑140 (MQ=255)                                                              cGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCCTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCg                                         >  1:196054/1‑140 (MQ=255)                                                                aTGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCCTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGAGTCCTTCAACCCGATGTAGGTGGCGCGCGGGCACGCGGTGATGGCGaa                                       >  2:397878/1‑140 (MQ=255)                                                                     cGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCCTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAAcggcg                                  <  2:581602/140‑1 (MQ=255)                                                                       tCGACTACGCTGCAGCATATCTCCAGCGCGTCGGCCTCCCGGCACAAAACATTGTCCAGACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACggcggc                                <  1:397878/140‑1 (MQ=255)                                                                              cGCTGCAGAATATCTGCAACGCTTCGGCCTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCtgg                         >  1:425966/1‑140 (MQ=255)                                                                              cGCTGCAGAATATCTGCAACGCCTCCGCCCCCCGGCACAAAACATTGTCGACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCtgg                         <  1:261172/140‑1 (MQ=255)                                                                                      aaTATCTGCAACGCTTCAGCCTACCGGCACACAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCg                 <  2:329695/140‑1 (MQ=255)                                                                                       atatCTGCAACGCTTCGGCCTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGt                <  2:196054/140‑1 (MQ=255)                                                                                                  gCTTCGGCCTCCCGGCACAAAACAGTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGc     >  2:579541/1‑140 (MQ=255)                                                                                                    ttCGGCCTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGCACGGCGGATTCCTGGTGGACCGGTGGTTTATCAGCaa   >  1:298929/1‑140 (MQ=255)                                                                                                     tCGGCCTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCaaa  >  1:93448/1‑140 (MQ=255)                                                                                                          |                                                                                                                                       ATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAA  >  NC_000913/3524413‑3524652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑