| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 329,082 | C→T | E85K (GAG→AAG) | betI ← | DNA‑binding transcriptional repressor BetI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 329,082 | 0 | C | T | 97.7% | 145.9 / ‑4.3 | 44 | E85K (GAG→AAG) | betI | DNA‑binding transcriptional repressor BetI |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (23/20); total (24/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
TAAACGATAGAGCATCGGCTGATGCATACTGCTGGCCCAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTCTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCATGGTTGCTTCCAGCAGACCATTTTTGTCCCTGAAATAGTGGCTGATGATCCCCGTAGAAACGCC > NC_000913/328960‑329217 | tAAACGATAGAGCATCGGCTGAGGCGTACGGCTGGCCTAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGaa < 1:414980/140‑1 (MQ=255) aaaCGATAGAGCATCGGCTGATGCATACTGCTGGCCCAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAg < 2:75582/140‑1 (MQ=255) aaCGATAGAGCATCGGCTGATGCATACTGCTGGCCCAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTCCCGCCAACCATCACCTATAATCGCTACTTTACACTGCCCTGCGGAAgg > 2:414980/1‑139 (MQ=255) tAGAGCATCGGCTGATGCATACTGCTGGCCCAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGTCTGGAATAGCTGCTTTTCACTCCCCTGCGGAAGTGCATg > 2:273229/1‑140 (MQ=255) tAGAGCATCGGCTGATGCATACTGCTGGCCCAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATg > 1:772544/1‑140 (MQ=255) tAGAGCATCGGCTGATGCATACTGCTGGCCCAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCAAACAATCGACTGTAATAGCTGCTTTACACTGCCCTGCGGAAGTGCATg > 2:294136/1‑140 (MQ=255) agCATCGGCTGATGCATACTGCTGGCCCAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTaa > 1:644700/1‑140 (MQ=255) gCATCGGCTGATGCATACTGCTCGCTCAGTACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAAt < 1:276627/140‑1 (MQ=255) cATCGGCTGATGCATACTGCTGGCCCAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATc > 1:73158/1‑140 (MQ=255) cGGCTGATGCATACTGCTGGCCCAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGAt > 2:78120/1‑140 (MQ=255) aCTGCTGGCCCAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGt > 1:440860/1‑140 (MQ=255) ctgGCCCAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACg > 2:115716/1‑140 (MQ=255) ggCCCAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTCTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCa > 1:85097/1‑140 (MQ=255) gCCCAGAACGCCAGCGAGGCTTTCACCGCGGCACTGCACACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAg < 1:420983/140‑1 (MQ=255) gCCCAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGGACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACcg > 2:320480/1‑130 (MQ=255) gCCCAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACcg < 1:320480/130‑1 (MQ=255) gCCCAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAg > 1:483527/1‑140 (MQ=255) gCCCAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAg < 2:73158/140‑1 (MQ=255) cccAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGc > 1:84067/1‑140 (MQ=255) cccAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGc < 2:84067/140‑1 (MQ=255) gAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGAc > 1:142069/1‑140 (MQ=255) gAACGCCAGCCAGGCTTTCATCACCCCACCGCTCACCCGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGAc < 1:273229/140‑1 (MQ=255) cGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTgg < 2:771265/140‑1 (MQ=255) cagGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATc < 1:851664/140‑1 (MQ=255) gCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATcgcg < 2:168391/140‑1 (MQ=255) tttCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCa < 2:142069/140‑1 (MQ=255) tttCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCa < 2:777575/140‑1 (MQ=255) aTCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCATGGt > 1:93096/1‑140 (MQ=255) cTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCATGGTTGCTTCcag < 2:644700/140‑1 (MQ=255) cGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCATGGTTGCTTCCAGCAGACCAtttt > 2:846168/1‑140 (MQ=255) tttCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCATGGTTGCTTCCAGCAGACCATTTTTg > 2:217879/1‑140 (MQ=255) tttCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCATGGTTGCTTCCAGCAGACCATTTTTg > 2:669009/1‑140 (MQ=255) cATCGAAGTTTCCGCCAACAATCGCCTGTAACCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTAAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCATGGTTGCTTCCAGCAGACCATTTTTGTcc > 1:767637/1‑140 (MQ=255) tCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCATGGTTGCTTCCAGCAGACCATTTTTGTCCCt > 2:631293/1‑140 (MQ=255) aaGTTTCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCATGGTTGCTTCCAGCAGACCATTTTTGTCCCTGaa > 2:161595/1‑140 (MQ=255) tttCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCATGGTTGCTTCCAGCAGACCATTTTTGTCCCTGAAATa < 1:115716/140‑1 (MQ=255) tCCGCCAACAATCGGCTGTAATCGCTGATTTGCACTGCCCTGCGGCAGCGCATGTAATCGATTAAAAACCGCGTCACGCAGCTGACCGCTGATATCGCGCATGGTTGCTTCCAGCAGACCATTATTGTCCCTGCAATAgg > 2:113758/1‑139 (MQ=255) tCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCATGGTTGCTTCCAGCAGACCATTTTTGTCCCTGAAATAGt < 2:93096/140‑1 (MQ=255) tCCGCCAACAATCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCATGGTTGCTTCCAGCAGACCATTTTTGTCCCTGAAATAGt < 1:846168/140‑1 (MQ=255) cGCCAACAATCGCCTGTAATCGATGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCATGGTTGCTTCCAGCAGACCATTTTTGTCCCTGAAATAGTgg < 1:294136/140‑1 (MQ=255) aTCGCCTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCATGGTTGCTTCCAGCAGACCATTTTTGTCCCTGAAATAGTGGCTgatgat < 2:483527/140‑1 (MQ=255) ccTGTAATCGCTGCTTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCATGGTTGCTTCCAGCAGACCATTTTTGTCCCTGAAATAGTGGCTGATGATcccc < 1:217879/140‑1 (MQ=255) gctgctTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCATGGTTGCTTCCAGCAGACCATTTTTGTCCCTGAAATAGTGGCTGATGATCCCCGTAGAAACg > 1:620953/1‑140 (MQ=255) tgctTTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCATGGTTGCTTCCAGCACACCATTTTGGTCACTGGAATAGTGGGTGATAATCCCCGTAGAAACGcc > 2:236185/1‑140 (MQ=255) | TAAACGATAGAGCATCGGCTGATGCATACTGCTGGCCCAGAACGCCAGCCAGGCTTTCATCGCCGCACTGCTCACCTGCGTTTCATCGAAGTTTCCGCCAACAATCGCCTGTAATCGCTGCTCTGCACTGCCCTGCGGAAGTGCATGTAATCGATTCAAAACCGCGTCACGCAGCTGACTGGTGATATCGCGCATGGTTGCTTCCAGCAGACCATTTTTGTCCCTGAAATAGTGGCTGATGATCCCCGTAGAAACGCC > NC_000913/328960‑329217 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |