Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,569,795 T→C T509A (ACG→GCG)  glgX ← limit dextrin alpha‑1,6‑glucohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,569,7950TC100.0% 143.7 / NA 43T509A (ACG→GCG) glgXlimit dextrin alpha‑1,6‑glucohydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/20);  total (23/20)

ACGCGGCGGTAAATGCGGTTAAACCACTGCTTGCCTGCGACCAGTCCAACCAGGTTAATTGGTTATCCTGACAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGTACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAACCGCCTAACCCTTCTTTACCATGATTGTTACTGTAATTGTTGTTGGTCCCGTCGCG  >  NC_000913/3569660‑3569924
                                                                                                                                       |                                                                                                                                 
aCGCGGCGGTAAATGCGGTTAAACCACTGCTTGCCTGCGACCAGTCCAACCAGGTTAATTGGTTATCCTGACAGTAGGCATTGTTATTGCCGTGCTGGCTCTGAACATGTTCGTCACAGGCCGGTAACATCGGCGCAccc                                                                                                                               >  2:239396/1‑140 (MQ=255)
  gcggcgGTAAATGCGGTTAAACCACTGCTTGCCTGCGACCAGTCCAACCAGGTTAATTGGTTATCCTGACAGTAGGCATTGTTATTGCCATTCTGGCTGTGACCTTGTGCGTCACCGGCCAGAAACATCGGCGCACCCTg                                                                                                                             >  2:246536/1‑140 (MQ=255)
   cggcggTAAATGCGGTTAAACCACTGCTTGCCTGCGACCAGTCCAACCAGGTTAATTGGTTATCCTGACAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTgg                                                                                                                            <  1:789037/140‑1 (MQ=255)
     gcggTAAATGCGGTTAAACCACTGCTTGCCTGCGACCAGTCCAACCAGGTTAATTGGTTATCCTGACAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGga                                                                                                                          <  1:189162/140‑1 (MQ=255)
     gcggTAAATGCGGTTAAACCACTGCTTGCCTGCGACCAGTCCAACCAGGTTAATTGGTTATCCTGACAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGga                                                                                                                          >  1:544996/1‑140 (MQ=255)
      cggTAAATGCGGTTAAACCACTGCTTGCCTGCGACCAGTCCAACCAGGTTAATTGGTTATCCTGACAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGgag                                                                                                                         >  2:633606/1‑140 (MQ=255)
             tGCGGTTAAACCACTGCCTGCCTGCGACCAGTCCAACCAGGTTAATTGGTTATCCTGACAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGcaaca                                                                                                                  <  1:642788/140‑1 (MQ=255)
                 gTTAAACCACTGCTTGCCTGCGACCAGTCCAACCAGGTTAATTGGTTATCCTGACAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACgt                                                                                                              <  1:633606/140‑1 (MQ=255)
                  ttAAACCACTGCTTGCCTGCGACCAGTCCAACCAGGTTAATTGGTTATCCTGACAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGgag                                                                                                                         >  2:496317/1‑128 (MQ=255)
                  ttAAACCACTGCTTGCCTGCGACCAGTCCAACCAGGTTAATTGGTTATCCTGACAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGgag                                                                                                                         <  1:496317/128‑1 (MQ=255)
                     aaCCACTGCTTGCCTGCGACCAGTCCAACCAGGTTAATTGGTTATCCTGACAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACgttgtt                                                                                                          <  2:605597/140‑1 (MQ=255)
                          cTGCTTGCCTGCGACCAGTCCACCCCGGTCAATTGGTCATCCTGACAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAg                                                                                                     <  1:246536/140‑1 (MQ=255)
                                            tCCAACCAGGTTAATTGGTTATCCTGACAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTcgcg                                                                                   >  2:32123/1‑140 (MQ=255)
                                              cAACCAGGTTAATTGGTTATCCTGACAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGcgcc                                                                                 <  2:680074/140‑1 (MQ=255)
                                              cAACCAGGTTAATTGGTTATCCTGACAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGcgcc                                                                                 <  2:662927/140‑1 (MQ=255)
                                                 ccAGGTTAATTGGTTATCCTGACAGTAGGCATTGTTATTACCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCgccgcc                                                                              <  1:850489/140‑1 (MQ=255)
                                                        aaTTGGTTATCCTGACATTAGGAATTGTTATTGCCTTGCTGGCTGTGAGCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAAc                                                                       <  2:288156/140‑1 (MQ=255)
                                                               tATCCTGACAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCa                                                                >  2:627666/1‑140 (MQ=255)
                                                                aTCCTGACAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCaa                                                               >  2:610428/1‑140 (MQ=255)
                                                                    tGACAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAgaga                                                           <  2:104414/140‑1 (MQ=255)
                                                                     gatacagggCATTGTTAGTGCCCTGCTGGCTATGATCATTTTCGTCACTGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGaa                                                          <  2:300408/133‑1 (MQ=255)
                                                                       cAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAAcc                                                        >  2:770749/1‑140 (MQ=255)
                                                                           aGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAACCGCCt                                                    <  1:770749/140‑1 (MQ=255)
                                                                            ggCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAACCGCCTa                                                   >  1:828545/1‑140 (MQ=255)
                                                                            ggCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTATCGCGCCGCCGATCTACCAGGACAAGAGAACCGCCTa                                                   >  1:219041/1‑140 (MQ=255)
                                                                            ggCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCAGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCACCGATCGACCAGGACAAGAGAACCGCCTa                                                   >  1:370460/1‑140 (MQ=255)
                                                                              cATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAACCGCCTAAc                                                 <  1:610428/140‑1 (MQ=255)
                                                                                ttGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCACCCGGGTCAACAGAACCGCCTAAccc                                               >  1:319624/1‑140 (MQ=255)
                                                                                     aTTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAACCGCCTAACCcttctt                                          <  1:786146/140‑1 (MQ=255)
                                                                                       tGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCTGGTTAAGAGAACCCCCTAAcccctgtctc                                        >  1:300408/1‑133 (MQ=255)
                                                                                        gCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAACCGCCTAACCCTTCTTTAc                                       >  2:554925/1‑140 (MQ=255)
                                                                                        gCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGGCGCGCCGCCGTTAAACCAGGTCAAGAGAACAGCCTAACCCTTCTTTAc                                       >  2:332213/1‑140 (MQ=255)
                                                                                         ccATGCTGGCTGTGACCCTGTTGGTCCCCGGTCAGCAACATCAGCGCATCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAACCGCCTAACCCTTCTTTAcc                                      <  2:370460/140‑1 (MQ=255)
                                                                                           aTGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAACCGCCTAACCCTTCTTTACCAt                                    >  1:748947/1‑140 (MQ=255)
                                                                                             gctggctgTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAACCGCCt                                                    >  1:134784/1‑122 (MQ=255)
                                                                                             gctggctgTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAACCGCCt                                                    <  2:134784/122‑1 (MQ=255)
                                                                                             gctggctgTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAACCGCCTAACCCTTCTTTACCATGa                                  >  2:690550/1‑140 (MQ=255)
                                                                                                 gctgTGACCATGTTCGTTACCGCCCAGTAACCTCGCCGCCCCCTGGGACAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAACCGCCTAACCCTTCTTTACCATGATTGt                              <  2:219041/140‑1 (MQ=255)
                                                                                                 gctgTGACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAACCGCCTAACCCTTCTTTACCATGATTGt                              <  1:627666/140‑1 (MQ=255)
                                                                                                    gtgACCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCACCGTTCAACCAGGTCAAGAGAACCGCCTAACCCTTCTTTACCATGATTGTTAc                           <  1:32123/140‑1 (MQ=255)
                                                                                                       aCCATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGTGAACCGCTTAAGCCTTCTTTATCATGATTGTTACTGt                        >  1:247386/1‑140 (MQ=255)
                                                                                                        ccATGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAACCGCCTAACCCTTCTTTACCATGATTGTTACTGTa                       >  2:827070/1‑140 (MQ=255)
                                                                                                           tGTTCGTCACCGGCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAACCGCCTAACCCTTCTTTACCATGATTGTTACTGTAAtt                    >  2:466585/1‑140 (MQ=255)
                                                                                                                       gCCAGTAACATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAACCGCCTAACCCTTCTTTACCATGATTGTTACTGTAATTGTTGTTGGTccc        >  1:653137/1‑140 (MQ=255)
                                                                                                                             aaCATCGGCGCACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAACCGCCTAACCCTTCTTTACCATGATTGTTACTGTAATTGTTGTTGGTCCCGTcgcg  <  1:827070/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                 
ACGCGGCGGTAAATGCGGTTAAACCACTGCTTGCCTGCGACCAGTCCAACCAGGTTAATTGGTTATCCTGACAGTAGGCATTGTTATTGCCATGCTGGCTGTGACCATGTTCGTCACCGGCCAGTAACATCGGCGTACCCTGGGAGAGCAACAACGTTGTTAACAGGGCGTGAATGCTGTCGCGCCGCCGTTCAACCAGGTCAAGAGAACCGCCTAACCCTTCTTTACCATGATTGTTACTGTAATTGTTGTTGGTCCCGTCGCG  >  NC_000913/3569660‑3569924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑