Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,586,429	T→C	N132S (AAC→AGC)	ggt ←	glutathione hydrolase proenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,586,429	0	T	C	100.0%	182.6 / NA	53	N132S (AAC→AGC)	ggt	glutathione hydrolase proenzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/22);  total (31/22)

TGCCAGTTTAAACGCGGGCTGCACGACTTTGTTCAGCGGCATGGTGCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGTATTGCCATTTTTCGAGCGGATTAACATAAAACCACCACCGCCCAGATTCCCTG  >  NC_000913/3586293‑3586555                                                                                                                                         |                                                                                                                               tGCCAGTTTAAACGCGGGCTGCACGACTTTGTTCAGCGGCATGGTGCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGc                                                                                                                             >  2:154009/1‑140 (MQ=255) tGCCAGTTTAAACGCGGGCTGCACGACTTTGTTCACCGGGATGGTGCCATATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGc                                                                                                                             <  2:336193/140‑1 (MQ=255)  gCCAGTTTAAACGCGGGCTGCACGACTTTGTTCAGCGGCATGGTGCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGcc                                                                                                                            <  1:491855/140‑1 (MQ=255)       tttAAACGCGGGCTGCACGACTTTGTTCAGCGGCATGGTGCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGat                                                                                                                       <  1:605775/140‑1 (MQ=255)         tAAACGCGGGCTGCACGACTTTGTTCAGCGGCATGGTGCCGTATTTATCCAGTGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGatca                                                                                                                     <  2:669439/140‑1 (MQ=255)           aaCGCGGGCTGCACGACTTTGTTCAGCGGCATGGTGCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGatcatc                                                                                                                   <  2:572152/140‑1 (MQ=255)             cgcgGGCTGCACGACTTTGTTCAGCGGCATGGTGCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGa                                                                                                                 <  1:154009/140‑1 (MQ=255)             cgcgGGCTGCACGACTTTGTTCAGCGGCATGGTGCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGa                                                                                                                 <  1:448002/140‑1 (MQ=255)                gggCTGCACGACTTTGTTCAGCGGCATGGTGCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGa                                                                                                              <  2:425150/140‑1 (MQ=255)                 ggCTGCACGACTTTGTTCAGCGGCATGGTGCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGaa                                                                                                             >  2:645671/1‑140 (MQ=255)                    tGCACGACTTTGTTCAGCGGCACGGTGCCGTTTTTCTCCCGCGCCAGCTAGAAACCTGCTATCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATATTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACat                                                                                                          <  2:368132/140‑1 (MQ=255)                            tttGTTCAGCGGCATGGTGCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCggg                                                                                                  >  1:658693/1‑140 (MQ=255)                              tGTTCAGCGGCATGGTGCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGAGTTTTTGCTTTCCGGGCAGCCCTGATGATCGTGGAACATATCGCGGGTc                                                                                                >  2:263752/1‑140 (MQ=255)                                     cGGCATGGTGCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTTCCCTGATTATCTAGGAACATAGCGCGGGTCGCTTTgg                                                                                         >  1:321649/1‑140 (MQ=255)                                      ggCATGGTGCCGTATTTATCCATCGCCAGTGAGAAACATGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGc                                                                                        <  1:263752/140‑1 (MQ=255)                                        cATGGTGCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGTCCTGATCCTCGAGGAAAATATCGCGGGTCGCTTTGGCgg                                                                                      >  2:316076/1‑140 (MQ=255)                                        cATGGTGCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCgg                                                                                      >  2:644692/1‑140 (MQ=255)                                          tGGTGCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGTGGAGCATATCGCGAGTCGCTTTGGCGGGt                                                                                    >  1:255911/1‑140 (MQ=255)                                          tGGTGCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCgggg                                                                                    >  2:573686/1‑139 (MQ=255)                                               ccGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCAt                                                                               >  2:130559/1‑140 (MQ=255)                                                cGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCAtt                                                                              <  2:625337/140‑1 (MQ=255)                                                cGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCAtt                                                                              <  1:693545/140‑1 (MQ=255)                                                       aTCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGGACAAATCTCGGGTCGCGTTGGAGGGTGCCATTTCGCGGa                                                                       >  2:297668/1‑140 (MQ=255)                                                       aTCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAAGATATCGCAGGTAGCATTGGCGTGTGCCATTTCGCGGa                                                                       >  1:254083/1‑140 (MQ=255)                                                       aTCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGa                                                                       >  1:332416/1‑140 (MQ=255)                                                            gcgccagcgAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCg                                                                  >  2:38096/1‑140 (MQ=255)                                                            gcgccagcgAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGCGCCATTTCGCCGAAATCg                                                                  >  2:195460/1‑140 (MQ=255)                                                            gcgccagcgAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCCCTTAGGCGGGTGCTATTTCGCGGAAATCg                                                                  >  1:332439/1‑140 (MQ=255)                                                             cgccagcgAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGa                                                                 >  1:813960/1‑140 (MQ=255)                                                              gccagcgAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCTATTTCGCGGAAATCGAt                                                                <  1:195460/140‑1 (MQ=255)                                                               ccagcgAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATa                                                               >  2:630053/1‑140 (MQ=255)                                                                 agcgAGAAACCTGCTACCGTACCCGGTGTGCAGGATGCCTGATGCGAACTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGc                                                             <  2:254083/140‑1 (MQ=255)                                                                   cgAGAAACCTGCTACCGGACCCCGTGTGCCGGAAGACAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCg                                                           <  2:332416/140‑1 (MQ=255)                                                                         aCCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGTAt                                                     >  2:613609/1‑140 (MQ=255)                                                                          ccTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGTAtt                                                    <  1:49417/140‑1 (MQ=255)                                                                                    tACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGTATTGCCATTTTTc                                          >  1:714081/1‑140 (MQ=255)                                                                                    tACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCAGTTCGCGGGAATGGAGAGCCGTCGTATTGCCATTTTTc                                          >  1:258451/1‑140 (MQ=255)                                                                                      cccGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGGATTGCCATTTTTCGa                                        >  2:483869/1‑140 (MQ=255)                                                                                      cccGGTGTGCCGGAAGCCAGATGCGAAGTAAGTGATTGTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGTATTGCCATTTTTCGa                                        <  1:396241/140‑1 (MQ=255)                                                                                       ccGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGTATTGCCATTTTTCGAg                                       <  1:38096/140‑1 (MQ=255)                                                                                       ccGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGTATTGCCATTTTTCGAg                                       <  1:130559/140‑1 (MQ=255)                                                                                           tgtgCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCCTTTCGCGGAAATCGATAGCCGTGGTACTGTCATTTTTCGAGCGGa                                   >  1:520853/1‑140 (MQ=255)                                                                                           tgtgCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTCCCATTTCGGGGAAATCGATAGCCGTGGTATTGCCATTTTTCGAGCGGa                                   >  2:357605/1‑140 (MQ=255)                                                                                               ccGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGACATCGATAGCCGTGGGATTGCCCTTTTTCGATCGGATTaa                               >  2:396241/1‑140 (MQ=255)                                                                                               ccGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGTATTGCCATTTTTCGAGCGGATTaa                               >  2:92795/1‑140 (MQ=255)                                                                                               ccGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGTATTGCCATTTTTCGAGCGGATTaa                               >  2:775194/1‑140 (MQ=255)                                                                                                cGGAAGCCAGATGCGAACTGAGTGATTTTTTTCTGGCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGTCATTTCGCGGAAATCGATAGCCGTGGTATTGCCATTTTTCGAGCGGATTAAc                              <  2:332439/140‑1 (MQ=255)                                                                                                          aTGCGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGTATTGCCATTTTTCGAGCGGATTAACATAAAaccac                    <  1:775194/140‑1 (MQ=255)                                                                                                             cGAAGTGAGTGATTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGTATTGCCATTTTTCGAGCGGATTAACATAAAaccaccac                 <  1:630053/140‑1 (MQ=255)                                                                                                                    agtgaTTTTTTGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGCGGTATTGCAATTATGCCGGCGGATTTACATATAACCACCGCCGCCCAg          >  2:398557/1‑140 (MQ=255)                                                                                                                       gaTTTTTTGCTGTCCGCGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCCTTTCGCGGAAATCGATAGCCGCGGGATTGCCATTTTTCGGGCGGATTAACATAAAAACACCACCGCCGAGGtt       >  2:846559/1‑140 (MQ=255)                                                                                                                            tttGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGTATTGCCATTTTTCGAGCGGATTAACATAAAACCACCACCGCCCAGATTCcctg  >  2:613582/1‑140 (MQ=255)                                                                                                                            tttGCTGTCCGGGCTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGGATTGCCATTTTTCGAGCGGATTAACATAAAACCACCACCGCCCAGATTCcctg  >  2:207326/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                               TGCCAGTTTAAACGCGGGCTGCACGACTTTGTTCAGCGGCATGGTGCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGTATTGCCATTTTTCGAGCGGATTAACATAAAACCACCACCGCCCAGATTCCCTG  >  NC_000913/3586293‑3586555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑