Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,601,749	A→G	V113A (GTT→GCT)	ftsX ←	cell division protein FtsX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,601,749	0	A	G	100.0%	187.1 / NA	54	V113A (GTT→GCT)	ftsX	cell division protein FtsX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/33);  total (21/33)

CAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTG  >  NC_000913/3601612‑3601880                                                                                                                                          |                                                                                                                                    caAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCa                                                                                                                                   <  1:109732/140‑1 (MQ=255)          accagTTACGGAACTCACCCAGTGCGTCTTCACGAGAAAGATAGTTAACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGc                                                                                                                          >  1:498518/1‑140 (MQ=255)            cagTTACGGAACTCACCCAGTGCGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCgg                                                                                                                        <  2:659744/140‑1 (MQ=255)              gTTACGGAACTCACCCAGTGCGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTg                                                                                                                      <  1:801735/140‑1 (MQ=255)              gTTACGGAACTCACCCAGTGCGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTACAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTg                                                                                                                      <  2:502971/140‑1 (MQ=255)                tACGGAACTCACCCAGTTCGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGAc                                                                                                                    <  2:341599/140‑1 (MQ=255)                tACGGAACTCACCCAGTGCGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTGTTTTGCAGATAAGCAGTGATTTGCGGTGAc                                                                                                                    <  2:74700/140‑1 (MQ=255)                 aCGGAACTCACCCAGTGCGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACg                                                                                                                   >  1:440271/1‑140 (MQ=255)                      aCTCACCCAGTGCGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTg                                                                                                                      >  2:46505/1‑132 (MQ=255)                      aCTCACCCAGTGCGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTg                                                                                                                      <  1:46505/132‑1 (MQ=255)                       cTCACCCAGTGCG‑CTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGa                                                                                                                 <  1:574862/135‑1 (MQ=255)                       cTCACCCAGTGCG‑CTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGa                                                                                                                 >  2:574862/1‑135 (MQ=255)                         cagccAGTGCGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGa                                                                                                                 >  2:494212/4‑134 (MQ=255)                         cACCCAGTGCGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGa                                                                                                                 <  1:494212/134‑1 (MQ=255)                         cACCCAGTGCGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATAc                                                                                                           >  2:849547/1‑140 (MQ=255)                         cACCCAGTGCGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATAc                                                                                                           >  2:112047/1‑140 (MQ=255)                          aCCCAGTGCGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACt                                                                                                          >  1:611602/1‑140 (MQ=255)                               gTGCGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTg                                                                                                                      <  2:125252/123‑1 (MQ=255)                               gTGCGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTg                                                                                                                      >  1:125252/1‑123 (MQ=255)                                  cGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTcgcc                                                                                                  >  1:778860/1‑140 (MQ=255)                                   gTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTcgccg                                                                                                 <  2:44844/140‑1 (MQ=255)                                    tCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTcgccgc                                                                                                >  1:776845/1‑140 (MQ=255)                                         aCGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGt                                                                                           <  2:674313/140‑1 (MQ=255)                                         aCGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGt                                                                                           <  2:7742/140‑1 (MQ=255)                                            agaAAGTTAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTaa                                                                                        >  2:120120/1‑140 (MQ=255)                                                 gATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCACCTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGttt                                                                                   <  1:824577/140‑1 (MQ=255)                                                    aGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTg                                                                                <  1:167385/140‑1 (MQ=255)                                                     gTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGt                                                                               >  1:843889/1‑140 (MQ=255)                                                      ttCGCTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTa                                                                              <  2:443180/140‑1 (MQ=255)                                                      ttCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCGCCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTa                                                                              <  1:112047/140‑1 (MQ=255)                                                      ttCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTa                                                                              <  2:778860/140‑1 (MQ=255)                                                      ttCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTa                                                                              <  1:849547/140‑1 (MQ=255)                                                      ttCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTa                                                                              <  2:776845/140‑1 (MQ=255)                                                      ttCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCAGCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTa                                                                              <  2:752451/140‑1 (MQ=255)                                                      ttCACTTTCACCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTa                                                                              <  2:494798/140‑1 (MQ=255)                                                      ttCACCTTCTCCACGCCTTGCTCAGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTa                                                                              <  2:591765/140‑1 (MQ=255)                                                       tCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTac                                                                             <  1:447910/140‑1 (MQ=255)                                                        cACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTaca                                                                            >  2:833752/1‑140 (MQ=255)                                                        aaCTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTcca                                                                            <  2:498518/139‑1 (MQ=255)                                                           tttCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCa                                                                         >  1:781239/1‑140 (MQ=255)                                                            ttCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCat                                                                        <  2:587302/140‑1 (MQ=255)                                                                 cACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAAc                                                                   <  1:120120/140‑1 (MQ=255)                                                                 cACGCCTTGCTCGGCCTGCAACAGTCCCACCACGCCCACAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAAc                                                                   <  1:224206/140‑1 (MQ=255)                                                                     ccTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGAc                                                               <  2:440271/140‑1 (MQ=255)                                                                         gCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTaca                                                                            >  1:179273/1‑123 (MQ=255)                                                                         gCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTaca                                                                            <  2:179273/123‑1 (MQ=255)                                                                         gCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATAATGCGTCGCTGCCGGGTAAACGTTTTAGTACACCATATAACAGACGCTg                                                           >  2:224206/1‑140 (MQ=255)                                                                           tCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTggg                                                         <  2:611602/140‑1 (MQ=255)                                                                            cGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGc                                                        <  2:843889/140‑1 (MQ=255)                                                                                         tgCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGaaa                                           >  1:76365/1‑140 (MQ=255)                                                                                              ccacGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACAGCATATAAGAGAGGCTGGGCAGTGTCAGAGAAATGGCg                                      >  1:274672/1‑140 (MQ=255)                                                                                                 cGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGAtg                                   >  1:681681/1‑139 (MQ=255)                                                                                                     cGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCa                               >  2:777/1‑140 (MQ=255)                                                                                                       cagcagCGTCATCGTCCAGCGTTTTTTGCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATc                             <  2:76365/140‑1 (MQ=255)                                                                                                                                  gCAGATAAGCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAATGTGGCGAACGGTTTg  <  2:781239/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    CAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACGAGAAAGATAGTTCACTTTCTCCACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTG  >  NC_000913/3601612‑3601880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑