Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,690,189	A→G	W25R (TGG→CGG)	bcsZ ←	endo‑1,4‑D‑glucanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,690,189	0	A	G	100.0%	132.7 / NA	40	W25R (TGG→CGG)	bcsZ	endo‑1,4‑D‑glucanase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/22);  total (18/22)

AGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGATTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCAGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAACACATTCATCTTCAATTACTCGTTATCCGGGTTAAGACGACGACGACTAATAATTCGCAGCAGACGCCACA  >  NC_000913/3690061‑3690323                                                                                                                                 |                                                                                                                                       aGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGATTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTg                                                                                                                             >  2:659595/1‑140 (MQ=255)    cgccAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGATTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCt                                                                                                                          <  1:480891/140‑1 (MQ=255)     gccAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGATTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTg                                                                                                                         >  2:163689/1‑140 (MQ=255)      ccAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGATTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGa                                                                                                                        >  1:194065/1‑140 (MQ=255)                    aTGCCGTAACTTTGCCCTTCGGAGGTGGTGATTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCTGGCGGGGTACAGACTGCGTGAACACTAAAGGcagc                                                                                                          >  2:238197/1‑140 (MQ=255)                             cTTTGCCCTTCGGAGGTGGTGATTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCCGCCAGATCACTAAAGGCAGCcagcagcag                                                                                                 >  1:256845/1‑140 (MQ=255)                                tGCCCTTCGGAGGTGGTGATTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCcagcag                                                                                                    >  1:762975/1‑134 (MQ=255)                                tGCCCTTCGGAGGTGGTGATTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCcagcag                                                                                                    <  2:762975/134‑1 (MQ=255)                                tGCCCTTCGGAGGTGGTGATTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCAt                                                                                              <  1:779030/140‑1 (MQ=255)                                  cccTTCGGAGGTGGTGATTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCg                                                                                            >  1:582707/1‑140 (MQ=255)                                        ggAGGTGGTGATTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGa                                                                                      <  1:515313/140‑1 (MQ=255)                                        ggAGGTGGTGATTTTCCGCGCGTCGTTGGTGTCGATGCCGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGa                                                                                      <  1:238197/140‑1 (MQ=255)                                             tggtgATTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCa                                                                                 <  1:659595/140‑1 (MQ=255)                                               gtgATTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGTTGCCTGAAGACTCAGGTGAGCCAGCAGCAGCTTCGTCACGATTCCACt                                                                               >  2:408125/1‑140 (MQ=255)                                                tgATTTTGCGCGCGTCGCTGGGCTCCATGTCGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTa                                                                              <  1:234416/140‑1 (MQ=255)                                                    tttGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCa                                                                          <  2:194065/140‑1 (MQ=255)                                                    tttGCGCGCGTCGCTGGCGTCGATGACGCGCACTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCa                                                                          <  2:220849/140‑1 (MQ=255)                                                      tGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAac                                                                        >  2:760176/1‑140 (MQ=255)                                                         gcgcgTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAAcaca                                                                     >  1:193490/1‑140 (MQ=255)                                                              tCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCaa                                                                         <  2:634763/131‑1 (MQ=255)                                                              tCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCaa                                                                         >  1:634763/1‑131 (MQ=255)                                                               cGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAACACATTCATc                                                               <  1:571322/140‑1 (MQ=255)                                                                               gcgcCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAACACATTCATCTTCAATTACTCGTTAt                                               <  2:193490/140‑1 (MQ=255)                                                                                             tgaTGTAATCCTTTTAAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAACACATTCATCTTCAATTACTCGTTATCCGGGTTAAgacga                                 <  1:394869/140‑1 (MQ=255)                                                                                               aTGTAATCCTTTTTAAATTGCTTCCAGGCAGACCGGGTATAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAACACATTCATCTTCAATTACTCGTTATCCGGGTTAAgacgacg                               <  2:270481/140‑1 (MQ=255)                                                                                               aTGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAACACATTCATCTTCAATTACTCGTTATCCGGGTTAAgacgacg                               <  2:582707/140‑1 (MQ=255)                                                                                               aTGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACAACGCAACAGATTAATCGTCAATTACACGTTATCCGGGTTAAgacgacg                               >  2:242190/1‑140 (MQ=255)                                                                                                 gTAATCCTTTTTAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAACACATTCACCTTGAATTACTCATTATCCGGGTTAAgacgacgac                             >  2:394869/1‑140 (MQ=255)                                                                                                          tttAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAACACATTCATCTTCAATTACTCGTTATCCGGGTTAAgacgac                                <  1:529441/128‑1 (MQ=255)                                                                                                          tttAAACTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAACACATTCATCTTCAATTACTCGTTATCCGGGTTAAgacgac                                >  2:529441/1‑128 (MQ=255)                                                                                                              aaCTGCTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAACACATTCATCTTCAATTACTCGTTATCCGGGTTAAGACGACGACGACTAATAATTCg                <  1:27893/140‑1 (MQ=255)                                                                                                                   cTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAACACATTCATCTTCAATTACTCGTTATCCGGGTAAAGACGACGACGAATAATAATTCgcagca           >  1:236040/1‑140 (MQ=255)                                                                                                                   cTCCCAGGCAGGCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAACAGCATCGTCACGATTCCACTACGCAACACATTCATCTTCAATTACTCGTTATCCGGGTTAAGACGACGACGACTAATAATTCgcagca           >  1:751089/1‑140 (MQ=255)                                                                                                                     cccAGGCAGGCCGGGTATAGGCCGCCTGAACTCTACAGGCAGCCAGCACCAGCATCGTCACGATTCCACTACGCAACACATTCATCTTCAATTACTCGTTATCCGGGTTAAGACGACGACGACTAATAATTCGCAGCAGa         <  2:236040/140‑1 (MQ=255)                                                                                                                       caggcaggCCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAACACATTCATCTTCAATTACTCGTTATCCGGGTTAAGACGACGACGACTAATAATTCGCAGCAGACg       <  1:25119/140‑1 (MQ=255)                                                                                                                            aggcCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAACACATTCATCTTCAATTACTCGTTATCCGGGTTAAGACGACGACGACTAATAATTCGCAGCAGACGCcaca  <  1:820000/140‑1 (MQ=255)                                                                                                                             ggcCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAACACATTCATCTTCAATTACTCGTTATCCGGGTTAAGACGACGACGACTAATAATTCGCAGCAGa         <  2:559584/132‑1 (MQ=255)                                                                                                                             ggcCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAACACATTCATCTTCAATTACTCGTTATCCGGGTTAAGACGACGACGACTAATAATTCGCAGCAGa         >  1:559584/1‑132 (MQ=255)                                                                                                                             ggcCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAACACATTCATCTTCAATTACTCGTTATCCGGGTTAAGACGACGACGACTAATAATTCGCAGCAGACGCcaca  <  1:847078/139‑1 (MQ=255)                                                                                                                             ggcCGGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAACACATTCATCTTCAATTACTCGTTATCCGGGTTAAGACGACGACGACTAATAATTCGCAGCAGACGCcaca  >  2:847078/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                       AGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGATTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCAGGTACAGGCTGCCTGAACACTAAAGGCAGCCAGCAGCAGCATCGTCACGATTCCACTACGCAACACATTCATCTTCAATTACTCGTTATCCGGGTTAAGACGACGACGACTAATAATTCGCAGCAGACGCCACA  >  NC_000913/3690061‑3690323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑