Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,731,244	A→G	E38E (GAA→GAG)	xylF →	xylose ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,731,244	0	A	G	100.0%	225.7 / NA	65	E38E (GAA→GAG)	xylF	xylose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/29);  total (36/29)

TACAGAAGGCCCTACACCATGAAAATAAAGAACATTCTACTCACCCTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAACGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAACACAAATGTCGCAGATTGAAAACATGATAAACCGGGGTGTCGA  >  NC_000913/3731113‑3731378                                                                                                                                    |                                                                                                                                       tACAGAAGGCCCTACACCATGAAAATAAAGAACATTCTACTCACCCTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCa                                                                                                                                <  1:855144/140‑1 (MQ=255)      aaGGCCCTACACCATGAAAATAAAGAACATTCTACTCACCCTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAg                                                                                                                           <  1:565596/140‑1 (MQ=255)        ggCCCTACACCATGAAAATAAAGAACATTCTACTCCCCCTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGAt                                                                                                                         <  2:568062/140‑1 (MQ=255)         gtccTACACCATGAAAATAAAGAACATTCTACTCACCCTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAg                                                                                                                           >  1:784080/3‑137 (MQ=255)         gCCCTACACCATGAAAATAAAGAACATTCTACTCACCCTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAg                                                                                                                           <  2:784080/137‑1 (MQ=255)           ccTACACCATGAAAATAAAGAACATTCTACTCACCCTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAg                                                                                                                           >  1:187427/1‑135 (MQ=255)           ccTACACCATGAAAATAAAGAACATTCTACTCACCCTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAg                                                                                                                           <  2:187427/135‑1 (MQ=255)                    tGAAAATAAAGAACATTCTACTCACCCTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCttt                                                                                                             >  1:10493/1‑140 (MQ=255)                    tGAAAATAAAGAACATTCTACTCACCCTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCttt                                                                                                             >  1:515395/1‑140 (MQ=255)                    tGAAAATAAAGAACATTCTACTCACCCTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCttt                                                                                                             >  2:779388/1‑140 (MQ=255)                    tGAAAATAAAGAACATTCTACTCACCCTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCGCCGTCTTAAGCACTAGCAAAAGGATCGAGATATCttt                                                                                                             >  1:242986/1‑140 (MQ=255)                         aTAAAGAACATTCTAGTCACCCATTGCACGTCACTCCAGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGaa                                                                                                        <  1:409958/140‑1 (MQ=255)                            aaGAACATTCTACTCACCCTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGGTCTCCGTCTTGAGCTCTGGCAAAAAGATCGAAATATCTTGGTGaaaaa                                                                                                     >  2:409958/1‑140 (MQ=255)                                 cATTCTACTCACCCTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAg                                                                                                >  2:146915/1‑140 (MQ=255)                                           aCCCTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCgg                                                                                      >  1:647967/1‑140 (MQ=255)                                           aCCCTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGAAATCGTTATGAAAAGGGCAGAATCTCTCgg                                                                                      >  1:231271/1‑140 (MQ=255)                                            cccTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGgc                                                                                     >  1:854150/1‑140 (MQ=255)                                            cccTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGgc                                                                                     >  1:606823/1‑140 (MQ=255)                                               tttGCACCTCACTCCTGTTTACTAACGTTGCTGCATACGCCAAAGAAGCCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGcgcg                                                                                  <  2:242986/140‑1 (MQ=255)                                                ttGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGa                                                                                 <  1:439470/140‑1 (MQ=255)                                                ttGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGa                                                                                 >  2:439470/1‑140 (MQ=255)                                                 tGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGaa                                                                                <  2:773749/140‑1 (MQ=255)                                                 tGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGaa                                                                                <  1:125939/140‑1 (MQ=255)                                                 tGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGaa                                                                                <  2:10493/140‑1 (MQ=255)                                                  gCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGaaa                                                                               >  1:457642/1‑140 (MQ=255)                                                       tCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTAtt                                                                          >  1:55208/1‑140 (MQ=255)                                                       tCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTAtt                                                                          >  2:179759/1‑140 (MQ=255)                                                         aCTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTg                                                                        >  1:114042/1‑140 (MQ=255)                                                           tCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTa                                                                      <  2:842013/140‑1 (MQ=255)                                                           tCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTa                                                                      <  2:114042/140‑1 (MQ=255)                                                           tCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTGGTGCAAACGGCAGAATATCTCCGCGCGAAAGTATTTGTa                                                                      >  2:259419/1‑140 (MQ=255)                                                           tCCTGCTTACCAACATTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTa                                                                      <  2:772608/140‑1 (MQ=255)                                                             cTGCTTACCAACGTTTCTGCACACGTCAACGAAGTCAGAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACa                                                                    <  1:373587/140‑1 (MQ=255)                                                             cTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACa                                                                    >  1:731913/1‑140 (MQ=255)                                                               gCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGt                                                                  <  1:779388/140‑1 (MQ=255)                                                                  tACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTg                                                               >  1:127029/1‑140 (MQ=255)                                                                   aCCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTa                                                                      <  1:636910/132‑1 (MQ=255)                                                                   aCCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTa                                                                      >  2:636910/1‑132 (MQ=255)                                                                   aCCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCACTCTGCTCGAAAGGATTTGTACAGTCTGc                                                              >  1:239135/1‑140 (MQ=255)                                                                    ccAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCa                                                             <  2:854150/140‑1 (MQ=255)                                                                     cAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAg                                                                              <  1:772113/122‑1 (MQ=255)                                                                     cAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAg                                                                              >  2:772113/1‑122 (MQ=255)                                                                       aCGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGCATCTCTCGGCGGGAGAGTATTAGTACAGTCTGCAAAt                                                          >  1:256287/1‑140 (MQ=255)                                                                       aCGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAAt                                                          >  2:735087/1‑140 (MQ=255)                                                                          ttGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGc                                                       >  1:537681/1‑140 (MQ=255)                                                                                  cacGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATgaaga                                               <  2:515395/140‑1 (MQ=255)                                                                                     gCCAAAGAAGTCATAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAac                                            >  1:53459/1‑140 (MQ=255)                                                                                       cAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAacac                                          <  2:510358/140‑1 (MQ=255)                                                                                       cAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAacac                                          >  2:596781/1‑140 (MQ=255)                                                                                         aagaagTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGGGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAACACaa                                        <  2:127029/140‑1 (MQ=255)                                                                                         aagaagTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAACACaa                                        <  2:537681/140‑1 (MQ=255)                                                                                               tCAAAATAGGTATGGCGCTTGATGATCTCCGTCTTCAGCGCTGGCAAATAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAACACAAATGTCg                                  <  2:254960/140‑1 (MQ=255)                                                                                               tCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGC‑‑‑CAAAAAGATCGAGTTATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAACACAAATGTCGCAg                               >  2:638109/1‑140 (MQ=255)                                                                                                cAAAATAGGTATGGCTATTGATGATTTCCGTCTTGAGGGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAACACAAATGTCGc                                 <  1:374811/140‑1 (MQ=255)                                                                                                cAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAACACAAATGTCGc                                 <  2:800034/140‑1 (MQ=255)                                                                                                        gTATGGCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGAAAATGGCAATGTAGAAACACAGATGTCGCAGATTGaa                         >  1:299934/1‑140 (MQ=255)                                                                                                             gCGATTGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGATGAAACAGAAATGTGGCAGATTGAAAACAt                    >  1:235117/1‑140 (MQ=255)                                                                                                                 ttGATGATCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAACACAAATGTCGCAGATTGAAAACATGATa                >  1:97155/1‑140 (MQ=255)                                                                                                                  tgatgaTCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAACACAAATGTCGCAGATTGAAAACATGATaa               >  1:71825/1‑140 (MQ=255)                                                                                                                    atgatCTCCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAACACAAATGTCGCAGATTGAAAACATGATAAAc             <  1:182200/140‑1 (MQ=255)                                                                                                                         ctcCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAACACAAATGTCGCAGATTGGAAACATGATAAACCgggg        <  1:118486/140‑1 (MQ=255)                                                                                                                         ctcCGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAACACAAATGTCGCAGATTGAAAACATGATAAACCgggg        <  1:657400/140‑1 (MQ=255)                                                                                                                            cGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCATTGAAGAAAGACAAATGTCGCAGATTTAAAACATGATAAACCGGGgtgt     >  2:352899/1‑140 (MQ=255)                                                                                                                            cGTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAACACAAATGTCGCAGATTGAAAACATGATAAACCGGGgtgt     <  2:606823/140‑1 (MQ=255)                                                                                                                             gTCTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAACACAAATGTCGCAGATTGAAAACATGATAAACCGGGGTGTc    >  1:807187/1‑140 (MQ=255)                                                                                                                               cTTGAGCGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAACACGAATGTCGGAGATTGAAGACATCATAAACCGGGGTGTCGa  >  2:264407/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                       TACAGAAGGCCCTACACCATGAAAATAAAGAACATTCTACTCACCCTTTGCACCTCACTCCTGCTTACCAACGTTGCTGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTCCGTCTTGAACGCTGGCAAAAAGATCGAGATATCTTTGTGAAAAAGGCAGAATCTCTCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAACACAAATGTCGCAGATTGAAAACATGATAAACCGGGGTGTCGA  >  NC_000913/3731113‑3731378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑