Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	330,537	T→C	F359L (TTT→CTT)	betT →	choline:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	330,537	0	T	C	100.0%	204.8 / NA	60	F359L (TTT→CTT)	betT	choline:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/33);  total (27/33)

GATGAATAACTGGACGCTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCTTCTACAGCCTGCTGGCGCAGTATC  >  NC_000913/330407‑330672                                                                                                                                   |                                                                                                                                        gATGAATAACTGGACGGTCTTCGTCTGGGCATGGTTGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCa                                                                                                                                <  2:374924/140‑1 (MQ=255) caggAATAACTGGACCCTCTTCATCCGGGGATGGTGGTTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCa                                                                                                                                <  1:254818/137‑1 (MQ=255)  aTGAATAACTGGACGCTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCAc                                                                                                                               <  1:648565/140‑1 (MQ=255)    gAATAACTGGACGCTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGc                                                                                                                             <  2:634913/140‑1 (MQ=255)    gAATAACTGGACGCTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGACACGTTAATTATTGCGCTTACCTTCACGc                                                                                                                             >  1:421007/1‑140 (MQ=255)        aaCTGGACGCTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCt                                                                                                                                   <  2:650796/130‑1 (MQ=255)        aaCTGGACGCTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCt                                                                                                                                   >  1:650796/1‑130 (MQ=255)        aaCTGGACGCTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGtt                                                                                                                         <  1:171479/140‑1 (MQ=255)         aCTGGACGCTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTa                                                                                                                        <  1:440943/140‑1 (MQ=255)            ggACGCTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTa                                                                                                                        >  2:84501/1‑137 (MQ=255)            ggACGCTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTa                                                                                                                        <  1:84501/137‑1 (MQ=255)               cGCTCTTCTTCTGGGCATGGTGGGTGGCATGGCCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTAGTGCTGGGGACGATGAGTATTCCGCGTACCGTCACGCTGTTATGGctg                                                                                                                  >  2:254818/1‑139 (MQ=255)                gCTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGctct                                                                                                                 <  2:825668/140‑1 (MQ=255)                   cttcttctGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCgg                                                                                                              <  1:467122/140‑1 (MQ=255)                   cttcttctAGGCATGGTCGGTGCCATGGTCGACGTATGTAGGCTTGTTACTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCgg                                                                                                              <  2:387184/140‑1 (MQ=255)                     tcttctGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGgtg                                                                                                            <  1:654647/140‑1 (MQ=255)                     tcttctGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGgtg                                                                                                            >  2:728919/1‑140 (MQ=255)                            ggCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCa                                                                                                     >  2:758198/1‑140 (MQ=255)                            ggCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTCACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCa                                                                                                     >  2:184839/1‑140 (MQ=255)                              cATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAAt                                                                                                   >  2:433713/1‑140 (MQ=255)                              cATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAAt                                                                                                   >  2:606358/1‑140 (MQ=255)                              cATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCCTACCTTCACGCGGTTATGGCTCTCGGTGTTCGGCAAt                                                                                                   >  1:349126/1‑140 (MQ=255)                                tggtggGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTGCCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAg                                                                                                 <  1:758198/140‑1 (MQ=255)                                 ggtgggtgGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAgc                                                                                                >  1:40672/1‑140 (MQ=255)                                  gtgggtggCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTTCCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAgcg                                                                                               <  2:649941/140‑1 (MQ=255)                                   tgggtggCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAgcgc                                                                                              >  1:627735/1‑140 (MQ=255)                                   tgggtggCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAgcgc                                                                                              >  1:109001/1‑140 (MQ=255)                                    gggtggCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAgcgcg                                                                                             <  1:606358/140‑1 (MQ=255)                                    gggtggCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAgcgcg                                                                                             <  1:28667/140‑1 (MQ=255)                                    gggtggCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTAGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAgcgcg                                                                                             <  1:439878/140‑1 (MQ=255)                                      gtggCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCt                                                                                           >  2:496592/1‑140 (MQ=255)                                      gtggCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCt                                                                                           >  1:671390/1‑140 (MQ=255)                                          cATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAgcgcg                                                                                             >  2:473759/1‑134 (MQ=255)                                          cATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAgcgcg                                                                                             <  1:473759/134‑1 (MQ=255)                                                   cGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAatcatc                                                                              >  2:764748/1‑140 (MQ=255)                                                   cGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACACTGTTATGTCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAatcatc                                                                              >  2:248548/1‑140 (MQ=255)                                                            gCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACggcggc                                                                     <  2:40672/140‑1 (MQ=255)                                                             cTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTTGGCAATACCGCACTATATGAAGTCATCCACGgcggcg                                                                    >  1:405244/1‑140 (MQ=255)                                                             cTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGgcggcg                                                                    >  1:865274/1‑140 (MQ=255)                                                                 ttCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGgcggcgcggc                                                                <  1:690949/140‑1 (MQ=255)                                                                  tCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCa                                                               <  1:20439/140‑1 (MQ=255)                                                                   ccTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCAt                                                              <  1:764748/140‑1 (MQ=255)                                                                            tATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAgg                                                     <  2:523871/140‑1 (MQ=255)                                                                              tctcGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTCTGAAATCCTCCTCGGCGGCGCAGCATTTGCCGAGGaa                                                   >  1:327118/1‑140 (MQ=255)                                                                                     tGGGCGTAGCATTCGCAGGTTCTTGCTGGGTGCGTTGATAATTCCGCTGACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATgg                                            <  2:405244/140‑1 (MQ=255)                                                                                            aCCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATcc                                     >  1:69067/1‑140 (MQ=255)                                                                                             ccATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCg                                    <  1:433713/140‑1 (MQ=255)                                                                                                 tCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAgc                                <  1:746937/140‑1 (MQ=255)                                                                                                 tCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTCACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAgc                                <  1:184839/140‑1 (MQ=255)                                                                                                 tCGCCAGTTCGTGCTGGCCACGTTGATTATTGCTCCTACTTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAgc                                <  2:421007/140‑1 (MQ=255)                                                                                                  cGCCAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAgcg                               >  2:792739/1‑140 (MQ=255)                                                                                                     cAGTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCgg                            <  1:728919/140‑1 (MQ=255)                                                                                                     cAGTTCGTGCTGGGCACATTGAATATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGAGCgg                            <  2:349126/140‑1 (MQ=255)                                                                                                       gTTCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCt                          >  2:28958/1‑140 (MQ=255)                                                                                                        ttCGTGCTGGGCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCtt                         >  2:62093/1‑140 (MQ=255)                                                                                                                gggCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCTTCTACAGcc                 <  2:65490/140‑1 (MQ=255)                                                                                                                  gCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCTTCTACAGCctg               >  1:510998/1‑140 (MQ=255)                                                                                                                  gCACGTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCTTCTACAGCctg               >  2:150760/1‑140 (MQ=255)                                                                                                                      gTTGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCTTCTACAGCCTGCTgg           >  2:175431/1‑140 (MQ=255)                                                                                                                        tGATTATTCCGCTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCTTCTACAGCCTGCTGgcg         <  2:510998/140‑1 (MQ=255)                                                                                                                               tCCGCTTGCCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCTTCTACAGCCTGCTGGCGCAGTATc  <  2:865274/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        GATGAATAACTGGACGCTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCTTCTACAGCCTGCTGGCGCAGTATC  >  NC_000913/330407‑330672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑