Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,763,972	A→G	N597S (AAC→AGC)	rhsA →	rhs element protein RhsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,763,972	0	A	G	100.0%	15.8 / NA	10	N597S (AAC→AGC)	rhsA	rhs element protein RhsA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/7);  total (3/7)

AAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGAT  >  NC_000913/3763936‑3764096                                     |                                                                                                                             aaGACACGCAGGGCCATGAAACGCGGTATGAATACAGCATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTa                       <  2:752226/140‑1 (MQ=11)     cacGCAGGGCCATGAAACGCGGTATGAATACAGCATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAAcgc                   >  1:196943/1‑140 (MQ=17)      gcGCAGGGCCATGAAACGCGGTATGAATACAGCATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAAcgcg                  <  2:694252/139‑1 (MQ=17)      ccGCAGGGCCATGAAACGCGGTATGAATACAGCGTCGCCGTTGGCCTGACCGCCGTCAGTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAAcgcg                  <  2:623470/139‑1 (MQ=11)      acGCAGGGCCATGAAACGGGGTATGAATACAGCATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAAcgcg                  <  1:166810/140‑1 (MQ=17)      acGCAGGGCCATGAAACGCGGTATGAATACAGCATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAAcgcg                  <  1:626247/140‑1 (MQ=18)      acGCAGGGCCATGAAACGCGGTATGAATACAGCATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAAcgcg                  <  1:694545/140‑1 (MQ=18)      acGCAGGGCCATGAAACGCGGTATGAATACAGCATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAAcgcg                  <  2:104482/140‑1 (MQ=17)      acGCAGGGCCATGAAACGCGGTATGAATACAGCATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAAcgcg                  <  2:507639/140‑1 (MQ=18)          aGGGCCATGAAACGCGGTATGAATACAGCATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGt              >  1:164545/1‑140 (MQ=17)                      cgcgGTATGAATACAGCATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGAt  >  2:624574/1‑140 (MQ=17)                                     |                                                                                                                             AAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGAT  >  NC_000913/3763936‑3764096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑