Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,773,042	T→C	G254G (GGT→GGC)	mtlA →	mannitol‑specific PTS enzyme IICBA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,773,042	0	T	C	100.0%	153.7 / NA	45	G254G (GGT→GGC)	mtlA	mannitol‑specific PTS enzyme IICBA component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/20);  total (25/20)

GGTAAATCAATCTTCTTCCTGATTGAAGCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTT  >  NC_000913/3772908‑3773175                                                                                                                                       |                                                                                                                                      ggTAAATCAATCTTCTTCCTGATTGAAGCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCAt                                                                                                                                     >  2:431653/1‑137 (MQ=255) ggTAAATCAATCTTCTTCCTGATTGAAGCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCa                                                                                                                                  >  1:24444/1‑140 (MQ=255) ggTAAATCAATCTTCTTCCTGATTGAAGCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCa                                                                                                                                  >  2:540131/1‑140 (MQ=255) ggTAAATCAATCTTCTTCCTGATTGAAGCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCa                                                                                                                                  >  1:623785/1‑140 (MQ=255)        caatcTTCTTCCTGATTGAAGCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATc                                                                                                                           <  2:450673/140‑1 (MQ=255)           tcttctTCCTGATTGAAGCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGAAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTAc                                                                                                                        >  2:824436/1‑140 (MQ=255)             ttcttcCTGATTGAAGCTAACCCAGGTCCATGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACtt                                                                                                                      <  1:523157/140‑1 (MQ=255)               cttcCTGATTGAAGCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTcc                                                                                                                    <  1:114647/140‑1 (MQ=255)                  ccTGATTGAAGCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGTAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGt                                                                                                                 <  1:591151/140‑1 (MQ=255)                    tGATTGAAGCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTAt                                                                                                               <  1:17639/140‑1 (MQ=255)                            gCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCtgat                                                                                                       >  2:10537/1‑140 (MQ=255)                                 cccAGGTCCAGGTATGGGCGTGGTGCTGGCGTACATGTTTTTTGGTCGCGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATc                                                                                                  <  2:310818/140‑1 (MQ=255)                                 cccAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATc                                                                                                  >  2:549920/1‑140 (MQ=255)                                              aTGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCt                                                                                     <  1:524831/140‑1 (MQ=255)                                                gggCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCg                                                                                   >  1:88566/1‑140 (MQ=255)                                                   cGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAg                                                                                <  1:540131/140‑1 (MQ=255)                                                     tgctgcTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTc                                                                              >  2:111279/1‑140 (MQ=255)                                                      gctgctGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCa                                                                             >  2:12719/1‑140 (MQ=255)                                                          ctgGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCt                                                                         <  1:111279/140‑1 (MQ=255)                                                            ggCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTcg                                                                       <  1:824436/140‑1 (MQ=255)                                                              cGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTcggc                                                                     <  2:623785/140‑1 (MQ=255)                                                                       tCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACt                                                            >  1:564356/1‑140 (MQ=255)                                                                       tCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATACACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCAt                                                                            >  1:571688/1‑124 (MQ=255)                                                                       tCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATACACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCAt                                                                            <  2:571688/124‑1 (MQ=255)                                                                          ttGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGc                                                         >  2:612568/1‑140 (MQ=255)                                                                           tGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCg                                                        >  1:635987/1‑140 (MQ=255)                                                                             gTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCgtg                                                      >  2:189086/1‑140 (MQ=255)                                                                                gTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTc                                                   >  2:26857/1‑140 (MQ=255)                                                                                  ggTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCAc                                                 <  2:635987/140‑1 (MQ=255)                                                                                  ggTAGCGCTAAACAGTCTGCGGGCGGTGCGACAATCATCCACTTCCTGGGGGGCATTCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCAc                                                 <  1:173751/140‑1 (MQ=255)                                                                                  ggTAGCGCTAAACAGTCTGCGGACGGTGCGGTAATCATCTACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTcgc                                                 <  2:345359/140‑3 (MQ=39)                                                                                            aaCAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATc                                       >  2:70044/1‑140 (MQ=255)                                                                                                 tCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTggg                                  >  2:587551/1‑140 (MQ=255)                                                                                                   tGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCg                                <  1:587551/140‑1 (MQ=255)                                                                                                           gTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTg                        >  2:661052/1‑140 (MQ=255)                                                                                                           gTGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTg                        >  1:59744/1‑140 (MQ=255)                                                                                                            tGCGGCAATCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTgg                       <  1:26857/140‑1 (MQ=255)                                                                                                                      atcCACTTCCTGGGGTGCATCCCCGCAATTTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTAGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGc             <  1:260393/140‑1 (MQ=255)                                                                                                                      atcCACTTCCTGGGGGGCATCCACGAAATCTACATCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGc             >  1:711219/1‑140 (MQ=255)                                                                                                                             tCCTGGGGGGCATCCACTAAATCTACTTCCCCTATTTGCAGATGAATCTGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCg      <  2:232661/140‑1 (MQ=255)                                                                                                                              ccTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGACATCTCCgg     >  1:721536/1‑140 (MQ=255)                                                                                                                                tGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTcc       <  2:100544/136‑1 (MQ=255)                                                                                                                                tGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTcc       >  1:100544/1‑136 (MQ=255)                                                                                                                                tGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGt   >  1:610826/1‑140 (MQ=255)                                                                                                                                 ggggggCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCCCGCTGACTATCCCGGGAGGTGGTCTGGTTTCGCCGGCATCTCCgggct  >  2:384400/1‑138 (MQ=255)                                                                                                                                       |                                                                                                                                      GGTAAATCAATCTTCTTCCTGATTGAAGCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTT  >  NC_000913/3772908‑3773175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑