Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,852,262 G→A Q175* (CAA→TAA)  ilvB ← acetohydroxy acid synthase I subunit IlvB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,852,2620GA98.2% 182.9 / NA 56Q175* (CAA→TAA) ilvBacetohydroxy acid synthase I subunit IlvB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (24/31);  minor base T (0/1);  total (24/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTGCACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGA  >  NC_000913/3852125‑3852394
                                                                                                                                         |                                                                                                                                    
gccgccCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTAca                                                                                                                                    <  1:526396/140‑1 (MQ=255)
gccgccCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGAGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTAca                                                                                                                                    >  2:203460/1‑140 (MQ=255)
gccgccCAGATAAAGAACCGGGCGTCTGGCAGCGTTACTCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTAca                                                                                                                                    <  1:226255/140‑1 (MQ=255)
  cgccCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTAcaca                                                                                                                                  >  1:179495/1‑140 (MQ=255)
  cgccCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTAcaca                                                                                                                                  >  1:752542/1‑140 (MQ=255)
  cgccCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTAca                                                                                                                                    >  1:192659/1‑138 (MQ=255)
  cgccCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTAca                                                                                                                                    <  2:192659/138‑1 (MQ=255)
         aTAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAg                                                                                                                           <  2:620816/140‑1 (MQ=255)
          tAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAgg                                                                                                                          <  1:790029/140‑1 (MQ=255)
          tAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAgg                                                                                                                          <  1:468860/140‑1 (MQ=255)
          tAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAgg                                                                                                                          <  2:489370/140‑1 (MQ=255)
           aaaGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGa                                                                                                                         <  1:482657/140‑1 (MQ=255)
              gCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATg                                                                                                                      >  1:816514/1‑140 (MQ=255)
              gCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATg                                                                                                                      <  2:752542/140‑1 (MQ=255)
                 ccGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGGCt                                                                                                                   >  1:802248/1‑140 (MQ=255)
                  cGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATg                                                                                                                      <  2:683825/136‑1 (MQ=255)
                  cGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATg                                                                                                                      >  1:683825/1‑136 (MQ=255)
                  cGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTa                                                                                                                  >  2:37964/1‑140 (MQ=255)
                   gggCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTAt                                                                                                                 >  1:654333/1‑140 (MQ=255)
                   gggCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTAt                                                                                                                 >  1:672351/1‑140 (MQ=255)
                   gggCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTAt                                                                                                                 >  1:198803/1‑140 (MQ=255)
                   gggCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTAt                                                                                                                 >  1:142326/1‑140 (MQ=255)
                   gggCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCACAAAAACTGCCGTTAACACATACTTAGGAATGTCTAt                                                                                                                 >  1:258658/1‑140 (MQ=255)
                   gggCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCACAAAAACGGCCATTGACACATCCTTAGGAATGTCTAt                                                                                                                 >  1:349171/1‑140 (MQ=255)
                   gggCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCACAAAAACCGCCGTTTACACATGCTTAGGAATTGCTAt                                                                                                                 >  1:366631/1‑140 (MQ=255)
                         ttGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCacac                                                                                                           <  1:61322/140‑1 (MQ=255)
                               gCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGcc                                                                                                     >  1:761742/1‑140 (MQ=255)
                                 gTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTg                                                                                                   >  1:515683/1‑140 (MQ=255)
                                          cgccgcTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGCTTACACATCCTAAGGAATGTCTATCCACACCGGGCCTGGGCGGcccg                                                                                          >  2:492402/1‑138 (MQ=255)
                                            ccgcTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGAt                                                                                        >  1:721650/1‑140 (MQ=255)
                                             cgcTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGAtt                                                                                       <  1:801798/140‑1 (MQ=255)
                                                  cGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCa                                                                                  <  1:37964/140‑1 (MQ=255)
                                                               ttcttcGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCAt                                                                     <  1:67917/140‑1 (MQ=255)
                                                                     gCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCa                                                               <  1:492402/140‑1 (MQ=255)
                                                                      cTAAAGGCGGGGGCGGCGGCTTGTTCTGCCAAAGCAGGCCGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCAt                                                              <  2:349171/140‑1 (MQ=255)
                                                                              gggggCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCAt                                                                     <  2:473547/125‑1 (MQ=255)
                                                                              gggggCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCAt                                                                     >  1:473547/1‑125 (MQ=255)
                                                                                 ggcggcggcTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGa                                                   >  1:560736/1‑140 (MQ=255)
                                                                                 ggcggcggcTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGa                                                   <  1:832895/140‑1 (MQ=255)
                                                                                 ggcggcggcTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGa                                                   >  1:728428/1‑140 (MQ=255)
                                                                                 ggcggcggcTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGa                                                   <  1:676710/140‑1 (MQ=255)
                                                                                 ggcggcggcTTTTTCTGCCATAGCGGGCTGTGTTTCAATCCCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGa                                                   <  2:560736/140‑1 (MQ=255)
                                                                                  ccggcggcTTTTTCTGCTATAGTGGGCTGTGGTTCTATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGAg                                                  <  2:366631/139‑1 (MQ=255)
                                                                                         cTTTTTCTGCCATAGCGTGCTGCGTTTCAATCTCACAAATTGCCGTTTTCACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGAGTTCTTCg                                           <  2:258658/140‑1 (MQ=255)
                                                                                          tttttCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGAGTTCTTCGa                                          <  1:188556/140‑1 (MQ=255)
                                                                                              tCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGAGTTCTTCGATATg                                      <  2:761742/140‑1 (MQ=255)
                                                                                               cTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGAGTTCTTCGATATGt                                     <  2:672351/140‑1 (MQ=255)
                                                                                                 gCCATAGCGGGCTGTTTTTCAACCTAAAAGACTGCCGGTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGAGTTCTTCGATATGTCt                                   <  1:390874/140‑1 (MQ=255)
                                                                                                   gaTAGCGGGCTGTGTTTCAATCTCAAAAACTACCGATTAAACATCCTTTGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGAGTTCTTCGATATGTCTGa                                 <  2:231336/139‑1 (MQ=255)
                                                                                                   cATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGAGTTCTTCGATATGTCTGa                                 <  2:198803/140‑1 (MQ=255)
                                                                                                      aGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGAGTTCTTCGATATGTCTGACCa                              <  2:142326/140‑1 (MQ=255)
                                                                                                      aGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGAGTTCTTCGATATGTCTGACCa                              <  2:721650/140‑1 (MQ=255)
                                                                                                               tgtTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACGTGCGGGAATTCGTCTATATGTATGACCAGATAGTtgc                     >  1:231336/1‑139 (MQ=255)
                                                                                                               tgtTTCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGAGTTCTTCGATATGTCTGACCAGATAGTtgt                     >  2:505322/1‑140 (MQ=255)
                                                                                                                  ttCAATCTCAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGtt                  <  2:179495/140‑1 (MQ=255)
                                                                                                                         tcAAAAACTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGAt           <  2:654333/140‑1 (MQ=255)
                                                                                                                                cTGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATa    <  2:816514/140‑1 (MQ=255)
                                                                                                                                 tGCCTTTTTGACATCCTTAGGAATGGCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCCAGGCGGAAGGCATCGCTCATGACCTGCGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGGGTTGGGGATaa   >  2:773171/1‑139 (MQ=255)
                                                                                                                                 tGCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGGGTTCTTCGATATTTCTGACCAGATACTTGTGTTTGGTGATGGGGATag   >  2:301138/1‑140 (MQ=255)
                                                                                                                                  gCCGTTTACACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATaga  >  2:801409/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                    
GCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATCTCAAAAACTGCCGTTTGCACATCCTTAGGAATGTCTATCCACACCGGGCCTGGGCGGCCTGATTGCGCAATGCGGAAGGCATCGCTCATGACCTGCGGGAGTTCTTCGATATGTCTGACCAGATAGTTGTGTTTGGTGATGGGGATAGA  >  NC_000913/3852125‑3852394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑