Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,854,735	A→G	T272A (ACG→GCG)	emrD →	multidrug efflux pump EmrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,854,735	0	A	G	100.0%	109.0 / NA	33	T272A (ACG→GCG)	emrD	multidrug efflux pump EmrD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/17);  total (16/17)

TGCTCCGGCGTGCTGATGGGCGCGGTGTTAGGGCTGAGCAGTATGACGGTCAGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTG  >  NC_000913/3854606‑3854858                                                                                                                                    |                                                                                                                            tGCTCCGGCGTGCTGATGGGCGCGGTGTTAGGGCTGAGCAGTATGACGGTCAGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAAtgtg                                                                                                                   <  2:635405/140‑1 (MQ=255) tGCTCCGGCGTGCTGATGGGCGCGGTGTTAGGGCTGAGCAGTATGACGGTCAGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAAtgtg                                                                                                                   >  1:635405/1‑140 (MQ=255)  gCTCCGGCGTGCTGATGGGCGCGGTGTTAGGGCTGAGCAGTATGACGGTCAGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTgg                                                                                                                  <  2:766312/140‑1 (MQ=255)   cTCCGGCGTGCTGATGGGCGCGGTGTTAGGGCTGAGCAGTATGACGGTCAGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGc                                                                                                                 >  1:335133/1‑140 (MQ=255)   cTCCCGCGTGCTGATGGGCGCGGTGGTAGGGCTGAGCAGTATGACGGTCAGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGc                                                                                                                 <  2:146838/140‑1 (MQ=255)     ccGGCGTGCTGATGGGCGCGGTGTTAGGGCTGAGCAGTATGACGGTCAGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCCCAATAAACGCTTCTCCGCGTTAATGTGGc                                                                                                                 <  2:107337/140‑1 (MQ=255)      cGGCGTGCTGATGGGCGCGGTGTTAGGGCTGAGCAGTATGACGGTCAGTATTTCGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGGTAATGTGGCAGt                                                                                                              >  2:756769/1‑140 (MQ=255)        gCGTGCTGATGGGCGCGGTGTTAGGGCTGAGCAGTATGACGGTCAGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCg                                                                                                            >  1:209762/1‑140 (MQ=255)          gTGCTGATGGGCGCGGTGTTAGGGCTGAGCAGTATGACGGTCAGTATTTCGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGt                                                                                                          <  1:756769/140‑1 (MQ=255)                aTGGGCGCGGTGTTAGGGCTGAGCAGTATGACGGTCAGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATTAACGCTTATCCGCGTTAATGTGGCAGTCGGTTATctg                                                                                                    >  1:395370/1‑140 (MQ=255)                     cgcgGTGTTAGGGCTGAGCAGTATGACGGTCAGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGctgcc                                                                                               >  2:859183/1‑140 (MQ=255)                     cgcgGTGTTAGGGCTGAGCAGTATGACGGTCAGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGctgcc                                                                                               >  1:818476/1‑140 (MQ=255)                              aGGGCTGAGCAGTATGACGGTCAGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTgctggctg                                                                                      <  1:859183/140‑1 (MQ=255)                                    gAGCAGTATGACGGTCAGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGc                                                                                >  1:712048/1‑140 (MQ=255)                                      gCAGTATGACGGTCAGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTg                                                                              <  2:815291/140‑1 (MQ=255)                                           aTGACGGTCAGTATTCTGTTTAGTCAGCCGATTCCGGGAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGAtgtg                                                                         <  1:360547/140‑1 (MQ=255)                                                ggTCAGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCTTGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATcc                                                                    <  2:335133/140‑1 (MQ=255)                                                  tCAGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATcccc                                                                  >  2:596729/1‑140 (MQ=255)                                                    aGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTg                                                                                 >  1:557890/1‑123 (MQ=255)                                                    aGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTg                                                                                 <  2:557890/123‑1 (MQ=255)                                                        ttttGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTgg                                                            >  2:493264/1‑140 (MQ=255)                                                           tGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGttt                                                         >  1:598381/1‑140 (MQ=255)                                                               tATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCCTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGTTGATGTGGATCCCCGACTGGTTTGGCg                                                     <  2:209762/140‑1 (MQ=255)                                                                       cGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAAt                                             >  1:545044/1‑140 (MQ=255)                                                                         aTTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGt                                           <  2:712048/140‑1 (MQ=255)                                                                          ttCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTc                                          >  2:568367/1‑140 (MQ=255)                                                                                   cGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACgctg                                 <  1:596729/140‑1 (MQ=255)                                                                                     ttttttGGCGCATGGTCTGCCGCACGT‑‑CCCACTTACCGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACgctgct                               <  2:395370/140‑1 (MQ=255)                                                                                             cgcATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCccg                       <  2:545044/140‑1 (MQ=255)                                                                                               cATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATACCCGACTGGTTTGGCGAGATGAATGACTGGACGCTGCTCGTTCccgcc                     >  2:322802/1‑140 (MQ=255)                                                                                                aTGGTTTGCCGGACGG‑‑CCCAATAAAAGCTCCTCCGCGATAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCccgccg                    <  1:322802/140‑1 (MQ=255)                                                                                                  ggTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCcgcg                  <  1:568367/140‑1 (MQ=255)                                                                                                            aCGT‑‑CCCAATAAACGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGAACGCTGCTTGTTCATGCCGAGCTGTTCtttt        >  2:392763/1‑140 (MQ=255)                                                                                                             cGT‑‑CCCAATAAGCGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCttttt       <  2:598555/140‑1 (MQ=255)                                                                                                                    cAATAAACGCTCCTCCGGGTTATTGTGGCAGTCGGCTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGCCGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTg  <  2:250644/140‑1 (MQ=255)                                                                                                                           cGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCttttt       <  2:546897/128‑1 (MQ=255)                                                                                                                           cGCTTCTCCGCGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCttttt       >  1:546897/1‑128 (MQ=255)                                                                                                                                    |                                                                                                                            TGCTCCGGCGTGCTGATGGGCGCGGTGTTAGGGCTGAGCAGTATGACGGTCAGTATTTTGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGT‑‑CCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTG  >  NC_000913/3854606‑3854858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑