Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	334,301	T→C	T45A (ACG→GCG)	yahB ←	putative LysR‑type DNA‑binding transcriptional regulator YahB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	334,301	0	T	C	100.0%	154.9 / NA	45	T45A (ACG→GCG)	yahB	putative LysR‑type DNA‑binding transcriptional regulator YahB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/24);  total (21/24)

CGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACGCGCAGCGGTAGTAAATGCCAACAGATTCTCTTCGGTAAAAATTGAGTTCATTA  >  NC_000913/334164‑334435                                                                                                                                          |                                                                                                                                       cGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGcct                                                                                                                                      >  2:782174/1‑140 (MQ=255) cGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGcct                                                                                                                                      >  2:39361/1‑140 (MQ=255)     aTAGTATCGATTCTGCGTTTGACGGTATATAAATCATCCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCAt                                                                                                                                  <  1:240194/140‑1 (MQ=255)      tAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACAGCGAGTGAACAGCATCACATCTAGCCCCGCCTCCATa                                                                                                                                 >  1:254297/1‑140 (MQ=255)       aGTATCGTTTCTGCGTTTGATGGCATAAAAATCATTCAGAAGGTCGCTAGGTTTGCGGAAAAAATACCGCCCGGACCCCGTTAACTCAATGCTGCGGGTCCTGCGAGTGAACAGCCCCACATCCGGCCCCGCCTCCATAc                                                                                                                                <  2:754687/140‑1 (MQ=255)       aGTATCGATTCTGCGTTTGATGTCACAAAAATCATTCCGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATAc                                                                                                                                <  1:318062/140‑1 (MQ=255)           tCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCtt                                                                                                                            >  2:511127/1‑140 (MQ=255)           tCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAG‑CCCGCCTCCATACGCTTa                                                                                                                           >  1:504563/1‑140 (MQ=255)           tCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCAAGTGAACTGCACCACATCCAGTCCCGCCTCCATACGCtt                                                                                                                            >  2:240194/1‑140 (MQ=255)             gATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTGGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCCCCACATCCAGCCCCGCCTCCATACGCTTaa                                                                                                                          <  2:100767/140‑1 (MQ=255)             gATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTaa                                                                                                                          <  1:469994/140‑1 (MQ=255)              aTTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAAt                                                                                                                         >  1:774284/1‑140 (MQ=255)               ttCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCtacacc                                                                                                                        >  1:181945/1‑135 (MQ=255)                tCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAG‑CCCGCCTCCATACGCTTAATGgt                                                                                                                      <  2:504563/140‑1 (MQ=255)                     gTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACAGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAg                                                                                                                  >  1:134967/1‑140 (MQ=255)                         gATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTaa                                                                                                              >  2:781841/1‑140 (MQ=255)                              cATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCg                                                                                                         >  2:724631/1‑140 (MQ=255)                              cATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGATAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCCCCATACGCTTAATGGTGTAGCTAATGGCg                                                                                                         >  1:698451/1‑140 (MQ=255)                                 aaaaaTCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCCCCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGaa                                                                                                      <  2:87211/140‑1 (MQ=255)                                 aaaaaTCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACGCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGaa                                                                                                      <  1:553008/140‑1 (MQ=255)                                  aaaaTCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAg                                                                                                     <  1:39361/140‑1 (MQ=255)                                          tCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGGCCCGCCTCCATAAGCTTAATGGTGTCGCTAGTGGCGGAAGTGGTTaaa                                                                                             >  2:333688/1‑140 (MQ=255)                                          tCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACAGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTaaa                                                                                             <  2:134967/140‑1 (MQ=255)                                              cagGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCa                                                                                         >  1:841379/1‑140 (MQ=255)                                                      tAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCGAATGGCGGAAGTGGTTAAACCCAACtcttct                                                                                 >  2:375728/1‑140 (MQ=255)                                                          tttGCGGAAAAAATACCGCCCGGACCCCGTTAACTCAATGCGGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCgg                                                                             <  2:175236/140‑1 (MQ=255)                                                          tttGCGGAAAAAATAACGCCCGGACT‑CGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGc                                                                            <  2:707034/140‑1 (MQ=255)                                                            tGCGGAAAAAATAACGCCCGGACTCCGTTAATTCAATGCGGCGGGTACCGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCt                                                                           <  2:348672/140‑1 (MQ=255)                                                                             ccGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCaaa                                                          >  1:826818/1‑140 (MQ=255)                                                                                  cTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCCCCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAAcgcg                                                     <  1:65220/140‑1 (MQ=255)                                                                                  cTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAAcgcg                                                     <  1:511127/140‑1 (MQ=255)                                                                                   accGTTACCTAAATGTTGCGGCTCCCGCGTGAGACCAACACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACgcgc                                                    <  1:333688/139‑1 (MQ=255)                                                                                    ccGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTACCAAAACGCGCa                                                   <  1:782174/140‑1 (MQ=255)                                                                                     cGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAAGTCTTCTCACGCGTTACTGAAGTCGCCAAAACGCGCAg                                                  >  1:309951/1‑140 (MQ=255)                                                                                     cGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGAGGCCTTCCTGAAGCTGCCAAAACGCGCAg                                                  >  1:270396/1‑140 (MQ=255)                                                                                      gTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACGCGCAGc                                                 >  2:75750/1‑140 (MQ=255)                                                                                           cTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAAcgcg                                                     >  2:505448/1‑131 (MQ=255)                                                                                           cTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAAcgcg                                                     <  1:505448/131‑1 (MQ=255)                                                                                               aTGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCATCTCTTCTGAGGCTTTAATGAAGCTGTCAAAACGCGCAGCGGTAGTaaa                                        >  2:272840/1‑140 (MQ=255)                                                                                                 gctgcGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACGCGCAGCgg                                               <  2:570374/131‑1 (MQ=255)                                                                                                 gctgcGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACGCGCAGCgg                                               >  1:570374/1‑131 (MQ=255)                                                                                                   tgcGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACGCGCAGCGGTAGTAAATGcc                                    <  2:841379/140‑1 (MQ=255)                                                                                                                    aaCAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACGCGCAGCGGTAGTAAATGCCAACAGATTCTCTTCGGt                   >  2:586197/1‑140 (MQ=255)                                                                                                                      cAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACGCGCAGCGGTAGTAAATGCCAACAGATTCTCTTCGGTaa                 <  1:781841/140‑1 (MQ=255)                                                                                                                                tcccaccccGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACGCGCAGCGGTAGTAAATGCCAACAGATTCTCTTCGGTAAAAATTGAGtt       <  1:482781/135‑1 (MQ=255)                                                                                                                                 ccAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACGCGCAGCGGTAGTAAATGCCAACAGATTCTCTTCGGTAAAAATTGAGTTc      <  2:711324/140‑1 (MQ=255)                                                                                                                                  cAGCCCCGCCTCCATTCGCTTATTGCTGTTGCTAATGCCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACGCGCAGCGGTAGTAAATGCCAACAGATTCTCTTCGGTAAAAATTGAGTTCa     <  1:272840/140‑1 (MQ=255)                                                                                                                                     ccccGCCTCCATACGCTTAATGGTGTAGTTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACGCGCAGCGGTAGTAAATGCCAACAGATTCTCTTCGGTAAAAATTGAGTTCATTa  >  2:197138/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       CGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACGCGCAGCGGTAGTAAATGCCAACAGATTCTCTTCGGTAAAAATTGAGTTCATTA  >  NC_000913/334164‑334435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑