Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,924,387	T→C	D416G (GAC→GGC)	mnmG ←	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,924,387	0	T	C	100.0%	89.5 / NA	29	D416G (GAC→GGC)	mnmG	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/10);  total (19/10)

GTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTGCGCGCGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGACCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCG  >  NC_000913/3924250‑3924518                                                                                                                                          |                                                                                                                                    gTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTGCGCGCGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGcca                                                                                                                                   <  1:778756/140‑1 (MQ=255)      tCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTGCGCGCGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAAc                                                                                                                              >  2:135828/1‑140 (MQ=255)          ccaccaGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTGCGCGCGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGt                                                                                                                          >  1:57832/1‑140 (MQ=255)          ccaccaGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTGCGCGCGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGt                                                                                                                          <  1:621011/140‑1 (MQ=255)                    cAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTGCGCGCGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGAt                                                                                                                <  1:102826/140‑1 (MQ=255)                                            aCGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTGCGCGCGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGccc                                                                                        <  1:135828/140‑1 (MQ=255)                                                    ccGCATTATCTTCGCGTAGCATCAGACGATATTCTGCGCGCGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACcttct                                                                                >  1:83365/1‑140 (MQ=255)                                                           aTCTTCGCGTAGCATCACACGAGTTTCTGCGAGCGGAGTCAACATACGTTACGGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTcag                                                                         <  2:325789/140‑1 (MQ=255)                                                            tCTTCGCGTAGCATCAGACGATATTCTGCGCGCGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACACCTGAGACCGTACCGGAGCCCGACCTTCTTTGTcagc                                                                        >  1:261585/1‑140 (MQ=255)                                                             cTTCGCGTAGCATCAGACGATATTCTGCGCGCGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTcagca                                                                       <  2:57832/140‑1 (MQ=255)                                                                      gCATCAGACGATATTCTGCGCGCGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACgg                                                              >  1:145170/1‑140 (MQ=255)                                                                        gacAGACGATATTCTGCCCGCGGAGTAAACAAACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGc                                                            <  2:272056/138‑1 (MQ=255)                                                                                 tatTCTGCGCGCGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAg                                                   >  1:733040/1‑140 (MQ=255)                                                                                 tatTCTGCGCGCGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAg                                                   >  1:150963/1‑140 (MQ=255)                                                                                        cgcgcgAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTGTTTGTCATCAGGCAGACGGGCGGCGTTAAGAcctgtc                                            >  1:272056/1‑136 (MQ=255)                                                                                         gcgcgAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGc                                                            >  1:634247/1‑123 (MQ=255)                                                                                         gcgcgAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGc                                                            <  2:634247/123‑1 (MQ=255)                                                                                          cgcgAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTATGTCAGCGGACGGATGGGCAGGGTTAAGACCGGCCAg                                          >  1:268372/1‑140 (MQ=255)                                                                                               aGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGACCGGCCAGCAAAc                                     >  2:750860/1‑140 (MQ=255)                                                                                                         cgatacgGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGACCGGCCAGCAAACCTTGCGCAGc                           >  2:721358/1‑140 (MQ=255)                                                                                                         cgatacgGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGACCGGCCAGCAAACCTTGCGCAGc                           >  1:627114/1‑140 (MQ=255)                                                                                                         cgatacgGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGACCGGCCAACAAACCTTGCGCAGc                           >  1:374806/1‑140 (MQ=255)                                                                                                           atacgGTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGACCGGCCAGCAAACCTTGCGCAGccg                         >  2:754098/1‑138 (MQ=255)                                                                                                            tacgGTTCTTTGGTTCCGAAAGTGCACAGGCCATCTACTAGTACGCCGCGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGACCGGCCAGCAAACCTTGCGCAGCGGc                        <  2:261585/140‑1 (MQ=255)                                                                                                                gTTCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGACCGGCCAGCAAACCTTGCGCAGCGGcttct                    >  1:637978/1‑140 (MQ=255)                                                                                                                 ttCTTTGGTTCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGACCGGCCAGCAAACCTTGCGCAGCGGcttctt                   >  2:628442/1‑140 (MQ=255)                                                                                                                          tCCTAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCATCGTTAAGAGCGGCCAGCAAACCTTGGGCAGCGGCTTCTTCGTAAACgg          >  2:377174/1‑140 (MQ=255)                                                                                                                             tAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGACCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAg       <  2:442998/140‑1 (MQ=255)                                                                                                                             tAAAGTGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGACCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAg       <  1:628442/140‑1 (MQ=255)                                                                                                                                  tGCACAGGCCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGACCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCg  >  1:701021/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                    GTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTGCGCGCGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGACCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCG  >  NC_000913/3924250‑3924518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑