Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,931,868	T→C	L185L (TTA→CTA)	kup →	K(+):H(+) symporter Kup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,931,868	0	T	C	98.3%	200.1 / NA	59	L185L (TTA→CTA)	kup	K(+):H(+) symporter Kup
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (29/29);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC  >  NC_000913/3931731‑3931992                                                                                                                                          |                                                                                                                             tGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTa                                                                                                                            <  2:673105/140‑1 (MQ=255) tGGATACCTGGATAGATCCCCTCTCAATTCTCGTTCTAACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTa                                                                                                                            <  1:279186/140‑1 (MQ=255)    aTACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGt                                                                                                                         >  1:617224/1‑140 (MQ=255)     tACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTa                                                                                                                        <  1:192803/140‑1 (MQ=255)     tACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTAAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTa                                                                                                                            >  1:563100/1‑136 (MQ=255)     tACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTAAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTa                                                                                                                            <  2:563100/136‑1 (MQ=255)            aTAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCattat                                                                                                                 >  2:169185/1‑140 (MQ=255)                ttCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGTGCTACGCAACATTATTGCt                                                                                                             >  1:216843/1‑140 (MQ=255)                ttCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCattatt                                                                                                                <  1:527136/137‑1 (MQ=255)                ttCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCattatt                                                                                                                >  2:527136/1‑137 (MQ=255)                ttCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCt                                                                                                             >  1:847033/1‑140 (MQ=255)                ttCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCt                                                                                                             >  1:445979/1‑140 (MQ=255)                ttCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCt                                                                                                             <  2:474215/140‑1 (MQ=255)                ttCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCt                                                                                                             <  2:70710/140‑1 (MQ=255)                  ccccTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTa                                                                                                                            >  2:715276/1‑123 (MQ=255)                  ccccTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTa                                                                                                                            <  1:715276/123‑1 (MQ=255)                  ccccTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTaa                                                                                                           <  2:445979/140‑1 (MQ=255)                  ccccTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTaa                                                                                                           <  2:847033/140‑1 (MQ=255)                     ctctCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAAccc                                                                                                        >  2:436146/1‑140 (MQ=255)                     ctctCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAAccc                                                                                                        >  2:29585/1‑140 (MQ=255)                          aaTTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGCTCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCatt                                                                                                                   >  2:828207/1‑124 (MQ=255)                          aaTTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCatt                                                                                                                   <  1:828207/124‑1 (MQ=255)                               tCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGtgctg                                                                                              >  2:121645/1‑140 (MQ=255)                                     tcACTTTATTATTTATGGTTCATAAACATGGCACGCCTATGGTCGGTAAGCTATTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATgcg                                                                                        <  2:337623/140‑1 (MQ=255)                                      cACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTAACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATgcgc                                                                                       <  1:743765/140‑1 (MQ=255)                                      cACTTTATTATTTATGATTCAAAAACATGGCACGGCTATCGTCGGTAAGCTGTTTTCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGTGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATgcgc                                                                                       <  2:286550/140‑1 (MQ=255)                                             ttatttatGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATcc                                                                                >  2:725856/1‑140 (MQ=255)                                                 ttatGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACGCGGTAGTGCTGCATGCGCTGAATCCAAtg                                                                            >  1:342633/1‑140 (MQ=255)                                                   atGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAAtgtg                                                                          >  1:820244/1‑140 (MQ=255)                                                   atGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAAtgtg                                                                          >  1:75037/1‑140 (MQ=255)                                                   atGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAAtgtg                                                                          >  1:68714/1‑140 (MQ=255)                                                   atGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTAGTGCAAACTCGGAAGTGCTGCAAGCGCTGAATCCAAtgtg                                                                          >  2:282275/1‑140 (MQ=255)                                                     gATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTggg                                                                        <  1:804940/140‑1 (MQ=255)                                                     gATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTggg                                                                        <  1:121645/140‑1 (MQ=255)                                                     gATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTggg                                                                        >  2:384807/1‑140 (MQ=255)                                                       ttCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCg                                                                      >  2:149615/1‑140 (MQ=255)                                                          aaaaaCATGGCACGGCTATGGTGGGAAAGTTGTTTGCTCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTg                                                                   <  1:282275/140‑1 (MQ=255)                                                          aaaaaCATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTg                                                                   <  1:725856/140‑1 (MQ=255)                                                          aaaaaCATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTg                                                                   >  2:834041/1‑140 (MQ=255)                                                                      cGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTATCCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCtt                                                       <  2:216843/140‑1 (MQ=255)                                                                            tGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATAc                                                 >  1:626264/1‑140 (MQ=255)                                                                                cGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACaaaa                                             <  2:68714/140‑1 (MQ=255)                                                                                cGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACaaaa                                             <  2:803912/140‑1 (MQ=255)                                                                                   tAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACgg                                          >  1:468757/1‑140 (MQ=255)                                                                                     aGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGtt                                        <  1:527472/140‑1 (MQ=255)                                                                                     aGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGtt                                        <  1:169185/140‑1 (MQ=255)                                                                                           ttGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCtttt                                  >  1:620127/1‑140 (MQ=255)                                                                                              cgcCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTAtt                               <  2:468757/140‑1 (MQ=255)                                                                                                 cGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCTTTTCTTCCTTGTATGCAAAACGGTTTCTTTTATTGCa                            >  1:312327/1‑140 (MQ=255)                                                                                                    tCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCa                            <  1:647325/137‑1 (MQ=255)                                                                                                    tCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTa                         >  1:178195/1‑140 (MQ=255)                                                                                                    tCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGATAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCa                            >  2:647325/1‑137 (MQ=255)                                                                                                     cATGCTGACCTGGTTCTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAg                        <  1:384807/140‑1 (MQ=255)                                                                                                      aTGGTGACCTGGTTTATGATTCCGATAGGTCTGGGGTTACGTAGCATTATTGCTACCCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAgg                       <  1:330770/140‑1 (MQ=255)                                                                                                      aTGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAgg                       <  2:620127/140‑1 (MQ=255)                                                                                                        gCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAgggg                     <  1:149615/140‑1 (MQ=255)                                                                                                             ccTGGTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgtg                <  2:820244/140‑1 (MQ=255)                                                                                                                 gTTTTTGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCATCCTTGAAAACAAAACGGTTTCTTTTATTGAATTAGGGGCAGTGgtgt            >  2:294449/1‑140 (MQ=255)                                                                                                                      tGATTCTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTcc       >  2:96176/1‑139 (MQ=255)                                                                                                                           cTGGCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATTCAAAACGGATTCATTTATTGCATTAGGGGCAGTGGTGTTATCGATTAc  >  2:391120/1‑140 (MQ=255)                                                                                                                              gCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGtgttg          <  2:488490/129‑1 (MQ=255)                                                                                                                              gCAGGACTGGGGCTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGtgttg          >  1:488490/1‑129 (MQ=255)                                                                                                                                          |                                                                                                                             TGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTAC  >  NC_000913/3931731‑3931992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑