Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,994,386 T→A intergenic (+51/‑136) yzcX → / → yifL protein YzcX/putative lipoprotein YifL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,994,3860TA98.5% 206.2 / NA 66intergenic (+51/‑136)yzcX/yifLprotein YzcX/putative lipoprotein YifL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (28/37);  minor base G (0/1);  total (28/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTATGATCAGGTTTACCGAGCGAGCATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAATCCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGTCTTCTCCTGCGATGATAGAAAGCAGAAAGCGATGAACTTTACAGGCAATCCAT  >  NC_000913/3994251‑3994520
                                                                                                                                       |                                                                                                                                      
ttATGATCAGGTTTACCGAGCGAGCATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCTATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAAcccc                                                                                                                                    <  2:584489/140‑1 (MQ=255)
   tGATCAGGTTTACCGAGCGAGCATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACg                                                                                                                                 >  1:60739/1‑140 (MQ=255)
      tCAGGTTTACCGAGCGATCATCCTCACGCTGGCGGAACTGAAAAAGACCACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGt                                                                                                                              <  2:251142/140‑1 (MQ=255)
      tCAGGTTTACCGAGCGAGCATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGt                                                                                                                              <  1:11704/140‑1 (MQ=255)
        aGGTTTACCGAGCGAGCATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTaa                                                                                                                            >  1:595216/1‑140 (MQ=255)
        aGGTTTACCGAGCGAGCATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTaa                                                                                                                            >  1:59269/1‑140 (MQ=255)
         ggTTTACCGAGCGAGCATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGTGGTGCCGAGCACAAAAGTACCAGCACAACAAAAAACCCCACGAGTAAg                                                                                                                           >  2:279822/1‑140 (MQ=255)
         ggTTTACCGAGCGAGCATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAg                                                                                                                           >  1:12804/1‑140 (MQ=255)
         ggTTTACCGAGCGAGCATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAg                                                                                                                           >  1:765505/1‑140 (MQ=255)
         ggTTTACCGAGCGAGCATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAg                                                                                                                           >  1:4814/1‑140 (MQ=255)
          gTTTACCGAGCGAGCATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGc                                                                                                                          >  1:830002/1‑140 (MQ=255)
           tttACCGAGCGAGCATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGt                                                                                                                              <  2:510860/135‑1 (MQ=255)
           tttACCGAGCGAGCATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGt                                                                                                                              >  1:510860/1‑135 (MQ=255)
            ttACCGAGCGAGCATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGt                                                                                                                        <  2:18695/140‑1 (MQ=255)
             tACCGAGCGAGCATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGtt                                                                                                                       >  1:647474/1‑140 (MQ=255)
              aCCGAGCGAGCATCCGCACGCTTACTGAAATAAAAAATACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTta                                                                                                                      <  1:371122/140‑1 (MQ=255)
               ccGAGCGAGCATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAATTACCAGCAAAAGATGAAACCCGACGAGTAAGCGTtat                                                                                                                     >  1:233906/1‑140 (MQ=255)
                   gcgagcATCCTCACGGTGACGGGACGAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTc                                                                                                                 <  1:353966/140‑1 (MQ=255)
                    cgagcATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACt                                                                                                                  <  2:623956/138‑1 (MQ=255)
                    cgagcATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACt                                                                                                                  >  1:623956/1‑138 (MQ=255)
                    cgagcATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAAAATAAAACCCCACGAGTAAGCGTTATACTCg                                                                                                                >  1:105849/1‑140 (MQ=255)
                    cgagcATCCTCACGCTGACGGAACAAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCg                                                                                                                >  2:798134/1‑140 (MQ=255)
                     gagcATCCTCACGCTCACGGAAGTATAAAAGACAACAGACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCgc                                                                                                               <  1:279822/140‑1 (MQ=255)
                       gcATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCgcag                                                                                                             <  1:658114/140‑1 (MQ=255)
                       gcATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCgcag                                                                                                             >  2:31940/1‑140 (MQ=255)
                            cTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCAttt                                                                                                        <  2:647474/140‑1 (MQ=255)
                                     aCGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACTCCACGAGGAAGCGTTATACTCTCAGCATTTCCTCACttt                                                                                               >  2:254860/1‑140 (MQ=255)
                                     aCGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACttt                                                                                               <  2:59269/140‑1 (MQ=255)
                                     aCGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACttt                                                                                               >  2:660148/1‑140 (MQ=255)
                                        gAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCa                                                                                            <  1:554378/140‑1 (MQ=255)
                                         aaCTAAAAAAGACAACATACAAACCACATTGCGATCGTGAATAAAGCCGTCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAg                                                                                           <  2:233906/140‑1 (MQ=255)
                                         aaCTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAg                                                                                           <  1:562425/140‑1 (MQ=255)
                                         aaCTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAg                                                                                           <  2:38369/140‑1 (MQ=255)
                                          aCTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGa                                                                                          <  1:773674/140‑1 (MQ=255)
                                                   gACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATaaa                                                                                 <  1:209977/140‑1 (MQ=255)
                                                     caacaaACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAaga                                                                               <  1:337903/140‑1 (MQ=255)
                                                      aacaaacaAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAagag                                                                              <  2:205141/140‑1 (MQ=255)
                                                                      tGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCTGCATTTCCCCACTTTTAAGACTTCAGAAAGAGTCGCTAAACgcttgct                                                              >  2:363495/1‑140 (MQ=255)
                                                                         gATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCtttt                                                           <  2:129223/140‑1 (MQ=255)
                                                                         gATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTACATAAAGAGTCGCTAAACGCTTGCtttt                                                           <  1:31940/140‑1 (MQ=255)
                                                                             gTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGt                                                       >  1:752/1‑140 (MQ=255)
                                                                              tGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGtc                                                      <  1:424841/140‑1 (MQ=255)
                                                                              tGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGtc                                                      >  2:424841/1‑140 (MQ=255)
                                                                              tGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGtc                                                      <  2:60739/140‑1 (MQ=255)
                                                                                cATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGtc                                                      <  2:192037/138‑1 (MQ=255)
                                                                                cATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGtc                                                      >  1:192037/1‑138 (MQ=255)
                                                                                  tAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGtcttct                                                  >  1:551093/1‑140 (MQ=255)
                                                                                   aaaGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGTCTtctc                                                 <  2:845524/140‑1 (MQ=255)
                                                                                     aGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGTCTTCTCCt                                               >  1:655054/1‑140 (MQ=255)
                                                                                           ccTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGTCTTCTCCTGCgatg                                         <  2:4814/140‑1 (MQ=255)
                                                                                                cgcgAGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGTCTTCTCCTGCGATGATaga                                    <  2:12804/140‑1 (MQ=255)
                                                                                                    aGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTCTTATGTGTTCTCCTGCGATGATagaaagc                                >  1:329677/1‑140 (MQ=255)
                                                                                                    aGGTGCCGATCACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGATTGGTTTTACGTATTCTCCTGCGATGATagaaagc                                >  2:253194/1‑140 (MQ=255)
                                                                                                              cACGAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTGTTAAGTCCTCTCCTGCTATGACAGAAAGCAGAAAGCGAt                      >  2:269378/1‑140 (MQ=255)
                                                                                                               aCGAAACTACCAGCATAACATATAACCCCCCGAGTAACCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGTCTTCTCCTGCGATGATAGAAAGCAGAAAGCGATg                     <  1:253194/140‑1 (MQ=255)
                                                                                                               aCGAAACTACCAGCAAAACATACAACCCCCCGAGTAACCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGTCTTCTCCTGCGATGATAGAAAGCAGAAAGCGATg                     <  1:269378/140‑1 (MQ=255)
                                                                                                                 gAAACTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGTCTTCTCCTGCGATGATAGAAAGCAGAAAGCGATGaa                   >  2:829436/1‑140 (MQ=255)
                                                                                                                   aaCTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGTCTTCTCCTGCGATGATAGAAAGCAGAAAGCGATGAACt                 >  2:841059/1‑140 (MQ=255)
                                                                                                                     cTACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGTCTTCTCCTGCGATGATAGAAAGCAGAAAGCGATGAACttt               <  2:752/140‑1 (MQ=255)
                                                                                                                      tACCAGCAAAACATAAAGCCCCGCGAGTAAGGGTTATACTCGCAGCATCTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGTCTTCTCCTGCGATGATAGAAAGCAGAAAGCGATGAACTTTa              <  2:329677/140‑1 (MQ=255)
                                                                                                                      tACCAGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGTCTTCTCCTGCGATGATAGAAAGCAGAAAGCGATGAACTTTa              <  2:830002/140‑1 (MQ=255)
                                                                                                                       aCCAGCAAAACATAAAACCCCAGGATTAATCGTTATATTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGTCTTCTCCTGCGATGATAGAAAGCAGAAAGCGATGAACTTTAc             <  1:416336/140‑1 (MQ=255)
                                                                                                                          aGCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGTCTTCTCCTGCGATGATAGAAAGCAGAAAGCGATGAACTTTACAgg          <  2:655054/140‑1 (MQ=255)
                                                                                                                          aGCAAAACATAAAACCCCACGACTACGCGCTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGTCTTCTCCTGCGATGATAGAAAGCAGAAAGCGATGAACTTTACAgg          <  1:254860/140‑1 (MQ=255)
                                                                                                                           gCAAAACATAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGTCTTCTCCTGCGATGATAGAAAGCAGAAAGCGATGAACTTTACAGGc         <  2:595216/140‑1 (MQ=255)
                                                                                                                                  aTAAAACCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGTCTTCTCCTGCGATGATAGAAAGCAGAAAGCGATGAACTTTACAGGCAATCCAt  <  1:841059/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TTATGATCAGGTTTACCGAGCGAGCATCCTCACGCTGACGGAACTAAAAAAGACAACAAACAAACCACATTGCGATAGTGCATAAAGCCATCCTGGCGCGAGGTGCCGATCACGAAACTACCAGCAAAACATAAATCCCCACGAGTAAGCGTTATACTCGCAGCATTTCCTCACTTTTCAGACTTCATAAAGAGTCGCTAAACGCTTGCTTTTACGTCTTCTCCTGCGATGATAGAAAGCAGAAAGCGATGAACTTTACAGGCAATCCAT  >  NC_000913/3994251‑3994520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑