Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,058,537	A→G	D44G (GAC→GGC)	bipA →	ribosome‑dependent GTPase, ribosome assembly factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,058,537	0	A	G	100.0%	201.0 / NA	60	D44G (GAC→GGC)	bipA	ribosome‑dependent GTPase, ribosome assembly factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/33);  total (27/33)

GTGATCGAAAAATTGCGTAATATCGCCATCATCGCGCACGTAGACCATGGTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGACTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCGGGGCACGCCGACTTCGGTGGTGAAGTTGAACGTGTAAT  >  NC_000913/4058407‑4058672                                                                                                                                   |                                                                                                                                        gtgATCGAAAAATTGCGTAATAACGCCATCATCGCGCTCGTAGACCATGGTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGa                                                                                                                                <  1:241232/140‑1 (MQ=255) gcgATCGAAAAATTGCGTAATATCGCCATCATCGCGCACGTAGACCATGGTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGa                                                                                                                                >  2:517658/3‑140 (MQ=255)   gATCGAAAACTTGAGTAATATCGCCATCATCGCGCACGTAGACCATGCTAAAACCACCCTCGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCAAACCCCAAGAGCGCGTGATGGGCTCCAACGAtt                                                                                                                              <  2:548756/140‑1 (MQ=255)   gATCGAAAAATTGCGTAATATCGCCATCATCGCGCACGTAGACCATGGTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGAtt                                                                                                                              <  1:720834/140‑1 (MQ=255)       gAAAAATTGCGTAATATCGCCATCATCGCGCACGTAGACCATGGTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGga                                                                                                                          >  1:596408/1‑140 (MQ=255)         aaaaTTGCGTAATATCGCCATCATCGCGCACGTAGACCATGGTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACACTCGTGCCGAAAGCCATGAGCGCGTTATGGGCTCCAACGATTTGgaga                                                                                                                        >  2:393185/1‑140 (MQ=255)              tGCGTAATATCGCCATCATCGCGCACGTAGACCATGGTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAagag                                                                                                                   <  1:725763/140‑1 (MQ=255)               gCGTAATATCGCCATCATCGCGCACGTAGACCATGGTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGACGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGc                                                                                                                  <  2:119798/140‑1 (MQ=255)                      atCGCCATCATCGCGCACGTAGACCATGGTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGTAt                                                                                                           >  2:427181/1‑140 (MQ=255)                               atcGCGCACGTAGACCATGGTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCt                                                                                                  >  2:725867/1‑140 (MQ=255)                                tcGCGCACGTAGACCATGGTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTc                                                                                                 <  2:761824/140‑1 (MQ=255)                                             ccATGGTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCg                                                                                    <  1:517658/140‑1 (MQ=255)                                                tGGTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAacac                                                                                      <  2:143867/135‑1 (MQ=255)                                                tGGTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAacac                                                                                      >  1:143867/1‑135 (MQ=255)                                                tGGTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTa                                                                                 >  1:582448/1‑140 (MQ=255)                                                tGGTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTa                                                                                 >  2:187269/1‑140 (MQ=255)                                                tGGTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTa                                                                                 >  1:756265/1‑140 (MQ=255)                                                  gTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGCGCGTGGGTTTAACATCCTCGCGAAAAACACCGCTATc                                                                               >  1:386559/1‑140 (MQ=255)                                                  gTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATc                                                                               >  1:78296/1‑140 (MQ=255)                                                  gTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGAGTGGGATTACCATCCTCGCGAAAAACACCGCTATc                                                                               >  2:276584/1‑140 (MQ=255)                                                   tAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTTCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCa                                                                              <  2:187535/140‑1 (MQ=255)                                                      aaCCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTa                                                                                 >  2:187336/1‑134 (MQ=255)                                                      aaCCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTa                                                                                 <  1:187336/134‑1 (MQ=255)                                                      aaCCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAAt                                                                           >  2:579825/1‑140 (MQ=255)                                                        ccaccCTGGTAGACAGGCTGCTCCAGCAATCCGGTACATTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATgg                                                                         <  1:393185/140‑1 (MQ=255)                                                         caccCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGa                                                                        <  2:582448/140‑1 (MQ=255)                                                         caccCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGa                                                                        <  1:725867/140‑1 (MQ=255)                                                          accCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGaa                                                                       <  1:579825/140‑1 (MQ=255)                                                            ccTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAACAGCGTGGGTTTATCATCCTCGCGAAGAACATCGCTATCAAATGGAATg                                                                     >  2:357982/1‑140 (MQ=255)                                                             cTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGa                                                                    >  1:833631/1‑140 (MQ=255)                                                              tGGTAGACAAGCTGCTCCAACATTCCGGTCCGTTCGATTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGAt                                                                   <  1:310295/140‑1 (MQ=255)                                                              tGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGTATTACCATCCTCGCGAAAAACTCCGCTATCAAATGGAATGAt                                                                   <  1:427181/140‑1 (MQ=255)                                                                 tAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTAc                                                                <  2:756265/140‑1 (MQ=255)                                                                          tgctCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAAc                                                       <  2:833631/140‑1 (MQ=255)                                                                          tgctCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAAc                                                       >  1:798596/1‑140 (MQ=255)                                                                          tgctCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAAc                                                       <  1:276584/140‑1 (MQ=255)                                                                          tgctCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAAc                                                       >  1:5230/1‑140 (MQ=255)                                                                          tgctCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAAc                                                       >  1:191208/1‑140 (MQ=255)                                                                               caacaaTCCGGTACGTTCGACTCTCGTTCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAAcgatgt                                                  <  1:728728/140‑6 (MQ=255)                                                                                          tACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCgg                                       >  2:736305/1‑140 (MQ=255)                                                                                            cGTTCGACTCTCGTGGCGAAACTCAGGAGTGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCgggg                                     <  1:357982/140‑1 (MQ=255)                                                                                               tCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCGGGGCAc                                  >  1:720406/1‑140 (MQ=255)                                                                                               tCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCGGGGCAc                                  >  2:71664/1‑140 (MQ=255)                                                                                               tCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCGGGGCAc                                  >  1:96621/1‑140 (MQ=255)                                                                                                  aCTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCgggg                                     <  2:434744/134‑1 (MQ=255)                                                                                                  aCTCTCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCgggg                                     >  1:434744/1‑134 (MQ=255)                                                                                                   ctctCGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCGGGGCACGCCg                              >  2:817778/1‑140 (MQ=255)                                                                                                     ctcGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCGGGGCACGCCGAc                            <  2:101043/140‑1 (MQ=255)                                                                                                       cGTGCCGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCGGGGCACGCCGACtt                          <  2:720406/140‑1 (MQ=255)                                                                                                            cGAAACCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCGGGGCACGCCGACTTCggtg                     <  2:191208/140‑1 (MQ=255)                                                                                                              aaaCCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCGGGGCACGCCGACTTCggtggt                   <  1:736305/140‑1 (MQ=255)                                                                                                              aaaCCCAAGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCGGGGCACGCCGACTTCggtggt                   <  1:776551/140‑1 (MQ=255)                                                                                                                   cAAGAGCGCGTGATGGGCTCCAGCGATTTGGAGTATGAGTGTGGGATTTCCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCGGGGCACGCCGACTTCGGTGGTGAAGt              <  2:379792/140‑1 (MQ=255)                                                                                                                    aaGAGCGCGTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCC‑GGGCACGCCGACTTCGGTGGTGAAGTTg            <  2:5230/140‑1 (MQ=255)                                                                                                                          gcgTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCGGGGCACGCCGACTTCGGTGGTGAAGTTGAACgt       >  1:119877/1‑140 (MQ=255)                                                                                                                           cgTGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCGGGGCACGCCGACTTCGGTGGTGAAGTTGAACgtg      >  2:524797/1‑140 (MQ=255)                                                                                                                             tGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCGGGGCACGCCGACTTCGGTGGTGAAGTTGAACGTGTa    <  2:677207/140‑1 (MQ=255)                                                                                                                             tGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCGGGGCACGCCGACTTCGGTGGTGAAGTTGAACGTGTa    <  1:71664/140‑1 (MQ=255)                                                                                                                             tGATGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCGGGGCACGCCGACTTCGGTGGTGAAGTTGAACGTGTa    <  2:770800/140‑1 (MQ=255)                                                                                                                               aTGGGCTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCGGGGCACGCCGACTTCGGTGGTGAAGTTGAACGTGTAAt  <  2:101126/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        GTGATCGAAAAATTGCGTAATATCGCCATCATCGCGCACGTAGACCATGGTAAAACCACCCTGGTAGACAAGCTGCTCCAACAATCCGGTACGTTCGACTCTCGTGCCGAAACCCAAGAGCGCGTGATGGACTCCAACGATTTGGAGAAAGAGCGTGGGATTACCATCCTCGCGAAAAACACCGCTATCAAATGGAATGATTACCGTATCAACATCGTTGATACCCCGGGGCACGCCGACTTCGGTGGTGAAGTTGAACGTGTAAT  >  NC_000913/4058407‑4058672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑