Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,155,010	A→G	T4A (ACG→GCG)	argC →	N‑acetylglutamylphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,155,010	0	A	G	100.0%	168.1 / NA	50	T4A (ACG→GCG)	argC	N‑acetylglutamylphosphate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/22);  total (28/22)

ATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATACGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCATATGAACATAACCGCTTTGACTGTTTCAGCGCAAAGCAATGATGCGGGAAAGTTAATCTCCGATTTGC  >  NC_000913/4154875‑4155145                                                                                                                                        |                                                                                                                                        aTCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCt                                                                                                                                     >  2:748311/1‑140 (MQ=255) aTCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCt                                                                                                                                     >  1:27043/1‑140 (MQ=255)        ttCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATtgtg                                                                                                                              >  2:711441/1‑140 (MQ=255)          cATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAGGGTGAATGGCCCCGCTGTTGAATGCGCTGATTGTggg                                                                                                                            >  1:305867/1‑140 (MQ=255)          cATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAG‑AGGTGAATACCCCGGAGGTTGAAGGCGCTGATTGTGGGt                                                                                                                           >  1:337510/1‑140 (MQ=255)           aTGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGt                                                                                                                           >  2:501330/1‑140 (MQ=255)            tGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTg                                                                                                                          >  1:517135/1‑140 (MQ=255)            tGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAAACATAAGAAGGTAAATCGCCCCGATTTTGAATGCGCTGATtgctgtct                                                                                                                          >  2:340568/1‑134 (MQ=255)             gCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGc                                                                                                                         >  1:475840/1‑140 (MQ=255)                               aaTACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGgcgc                                                                                                       <  1:501330/140‑1 (MQ=255)                                 tACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCat                                                                                                     <  1:748311/140‑2 (MQ=255)                                 tACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCag                                                                                                     <  2:137921/140‑1 (MQ=255)                                      tAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTa                                                                                                <  1:799656/140‑1 (MQ=255)                                      tAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTa                                                                                                <  2:27043/140‑1 (MQ=255)                                      tAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTa                                                                                                <  1:531430/140‑1 (MQ=255)                                       aaCGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAg                                                                                               <  1:711441/140‑1 (MQ=255)                                        aCGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGCTTGTGGGGGCCGGCTGCTACGATGGCGCAGAGCTAGt                                                                                              >  1:354053/1‑140 (MQ=255)                                        aCGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGt                                                                                              >  2:567838/1‑140 (MQ=255)                                        aCGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGt                                                                                              <  1:822995/140‑1 (MQ=255)                                           ttGAGCGTAATAAAACCCACCAACCGTAAGGAGAATGTTGTACGTTTAGCCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGAc                                                                                           <  2:354053/140‑1 (MQ=255)                                             gAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCt                                                                                         >  2:521454/1‑140 (MQ=255)                                              aGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTa                                                                                        >  1:627649/1‑140 (MQ=255)                                                cGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGGGCCAGCGGCTAAGCCGGCGCAAAGCTAGTGACCTATg                                                                                      >  1:270972/1‑140 (MQ=255)                                                  taataaAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTa                                                                                    >  1:11175/1‑140 (MQ=255)                                                    ataaAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGTGCTGAGTGTGGGTGCCAGTGGCAACGCTGGCGCAGATTTAGTGACATATGTaaa                                                                                  >  2:388992/1‑140 (MQ=255)                                                    ataaAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTaaa                                                                                  >  2:188350/1‑140 (MQ=255)                                                          cccACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGt                                                                                              <  1:836598/122‑1 (MQ=255)                                                          cccACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGt                                                                                              >  2:836598/1‑122 (MQ=255)                                                           ccaccaGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCAt                                                                           <  1:205102/140‑1 (MQ=255)                                                             accaGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATcc                                                                         >  1:843288/1‑140 (MQ=255)                                                             accaGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATcc                                                                         >  2:651714/1‑140 (MQ=255)                                                              ccaGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCg                                                                        <  2:475840/140‑1 (MQ=255)                                                                 gCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCat                                                                     <  1:651714/140‑1 (MQ=255)                                                                         gTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCATATGAACAt                                                             >  1:525290/1‑140 (MQ=255)                                                                          tGAATGTTTTACGTTTACCCTGCCAACCAGAAATAAG‑AGGTGAATAGCGCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCATATGAACATaa                                                           <  2:337510/140‑1 (MQ=255)                                                                            aaTGTTTTATGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCATATGAACATAAc                                                          >  1:76267/1‑140 (MQ=255)                                                                            aaTGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCATATGAACATAAc                                                          <  2:517135/140‑1 (MQ=255)                                                                            aaTGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTAGGTAGATCGCCATCAGCATATGAACATAAc                                                          >  1:413495/1‑140 (MQ=255)                                                                                ttttACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCATATGAACATAACCGCt                                                      <  1:567838/140‑1 (MQ=255)                                                                                ttttACCTTTATCTTGCAAACCACACATAATAAGGTGAATAGCCGTGATGTTGAATGGGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCATATGAACATAACCGCt                                                      <  1:541941/140‑1 (MQ=255)                                                                                   tACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCATATGAACATAACCGCTTTg                                                   >  1:8049/1‑140 (MQ=255)                                                                                     cGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCATATGAACATAACCGCTTTGAc                                                 <  2:8049/140‑1 (MQ=255)                                                                                               ggCAACCAGACATAAGAGGGTGAATAGCCCCGATGATGAATGCGCTGAGTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCATATGAACATAACCGCTTTGACTGTTTCAgcg                                       <  2:270972/140‑1 (MQ=255)                                                                                               ggCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCATATGAACATAACCGCTTTGACTGTTTCAgcg                                       <  1:188350/140‑1 (MQ=255)                                                                                               ggCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCATATGAACATAACCGCTTTGACTGTTTCAgcg                                       <  2:742863/140‑1 (MQ=255)                                                                                                          aTAAGAAGGTGAATAGCCCCGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCATATGAACATAACCGCTTTGACTGTTTCAGCGCAAAGCAatga                            >  1:162430/1‑140 (MQ=255)                                                                                                                          ccccGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCATATGAACATAACCGCTTTGACTGTTTCAGCGCAAAGCAATGATGCGGGAAAGTTAAtc            >  2:461819/1‑140 (MQ=255)                                                                                                                           cccGATGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCATATGAACATAACCGCTTTGACTGTTTCAGCGCAAAGCAATGATGCGGGAAAGTTAAtct           >  1:201055/1‑140 (MQ=255)                                                                                                                                tGTTGAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCATATGAACCTAACCGCTTTGACTGTTTCAGCGCAAAGCAATGATGCGGGAAAGTTAATCTCCGAt      <  1:821556/140‑1 (MQ=255)                                                                                                                                    gAATGCGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCATATGAACATAACCGCTTTGACTGTTTCAGCGCAAAGCAATGATGCGGGAAAGTTAATCTCCGATTTGc  >  2:61041/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        ATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAATACGCTGATTGTGGGTGCCAGCGGCTACGCTGGCGCAGAGCTAGTGACCTATGTAAATCGCCATCCGCATATGAACATAACCGCTTTGACTGTTTCAGCGCAAAGCAATGATGCGGGAAAGTTAATCTCCGATTTGC  >  NC_000913/4154875‑4155145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑