Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	364,824	G→A	T494T (ACC→ACT)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	364,824	0	G	A	100.0%	206.9 / NA	61	T494T (ACC→ACT)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/37);  total (24/37)

AAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATT  >  NC_000913/364697‑364958                                                                                                                                 |                                                                                                                                       aaaGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTccg                                                                                                                            >  1:603494/1‑140 (MQ=255)  agacagcAGCGGGCGCGTCTCTTCACGTAGCGAAAGCAATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTccgc                                                                                                                           <  1:420659/134‑1 (MQ=255)  aaGGATCAGCGGGTGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTccgc                                                                                                                           <  1:574605/140‑1 (MQ=255)  aaGGATCAGCGGGCGTGTCTCTCCAGGTATCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTccgc                                                                                                                           <  1:376891/140‑1 (MQ=255)  aaGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTccgc                                                                                                                           <  2:656085/140‑1 (MQ=255)  aaGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTccgc                                                                                                                           <  1:691913/140‑1 (MQ=255)       tCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCt                                                                                                                      <  2:723630/140‑1 (MQ=255)               cgcgTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATa                                                                                                                 >  2:746218/1‑137 (MQ=255)               cgcgTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATa                                                                                                                 <  1:746218/137‑1 (MQ=255)               cgcgTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTg                                                                                                              >  2:496771/1‑140 (MQ=255)                 cgTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGCCCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCa                                                                                                            <  2:375679/140‑1 (MQ=255)                  gTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCAc                                                                                                           >  2:658966/1‑140 (MQ=255)                   tctctcCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAAATAATATCGGTGGCCGTAGTGTCGGCTccgc                                                                                                                           >  2:810906/1‑124 (MQ=255)                   tctctcCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAAATAATATCGGTGGCCGTAGTGTCGGCTccgc                                                                                                                           <  1:810906/124‑1 (MQ=255)                   tctctcCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AGATAATATCAGTGGCTGGAGTGTCGGATCCGAGGCCTTCAAAcctgtct                                                                                                          >  2:420659/1‑134 (MQ=255)                      ctcCAGGTAGCGAAACCCATTTTTTGATCGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACcggg                                                                                                       <  1:413666/140‑1 (MQ=255)                           ggTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGa                                                                                                  <  1:717525/140‑1 (MQ=255)                            gTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGaa                                                                                                 >  1:501777/1‑140 (MQ=255)                              aGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAgg                                                                                               <  2:517049/140‑1 (MQ=255)                                cGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGAt                                                                                             >  2:475829/1‑140 (MQ=255)                                cGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTACACCGGGCGGGAAGGAt                                                                                             >  1:670113/1‑140 (MQ=255)                                  aaaGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCg                                                                                           <  2:501777/140‑1 (MQ=255)                                   aaGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGa                                                                                          <  1:703897/140‑1 (MQ=255)                                   aaGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGa                                                                                          <  2:701434/140‑1 (MQ=255)                                   aaGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCCTAGTGTCGGCTCCGCCCCCTTCATATTGCATCGGGCGGGAAGGATCGa                                                                                          >  2:361647/1‑140 (MQ=255)                                    aGCCATTTTTTGATGGACCATTCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGAc                                                                                         <  1:336111/140‑1 (MQ=255)                                          tttttGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGAttt                                                                                   >  2:437530/1‑140 (MQ=255)                                              tGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATc                                                                               <  1:658966/140‑1 (MQ=255)                                                   gACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCg                                                                          >  1:845975/1‑140 (MQ=255)                                                        tttCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACa                                                                     <  1:624188/140‑1 (MQ=255)                                                          tCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAgc                                                                   <  2:647165/140‑1 (MQ=255)                                                          tCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAgc                                                                   <  2:603494/140‑1 (MQ=255)                                                               ccaGCCGGGAAGGGCTGGTCTTGATCCACGCCCGCATACTTCGGGC‑AACTAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATCCTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGcgtc                                                              <  2:353716/139‑1 (MQ=255)                                                                   ccGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGa                                                          >  2:149517/1‑140 (MQ=255)                                                                   ccGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGa                                                          >  2:126506/1‑140 (MQ=255)                                                                   ccGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGa                                                          >  2:638295/1‑140 (MQ=255)                                                                      ggAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTACACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTa                                                       <  2:670113/140‑1 (MQ=255)                                                                      ggAAGGGCTGGTCTTCAACCACGCGCGCGTTGATCCGGC‑TAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTa                                                       <  2:423301/140‑1 (MQ=255)                                                                           ggCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGcc                                                  <  2:185699/140‑1 (MQ=255)                                                                             cTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCgg                                                >  2:195217/1‑139 (MQ=255)                                                                              tGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTg                                               >  1:545303/1‑140 (MQ=255)                                                                               ggTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTgg                                              >  1:634736/1‑140 (MQ=255)                                                                                gTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGc                                             >  1:726034/1‑140 (MQ=255)                                                                                  cTTCATCCACGCGCGCGTACATAGGGC‑AAATTAAAACGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCt                                           <  2:393389/140‑1 (MQ=255)                                                                                   ttCATCCACGCGCGCCTACATCCGGT‑AAATAATATCGTTGGCCGTAGTGTCGGCTCTGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTg                                          <  1:257706/140‑1 (MQ=255)                                                                                               cgcgTACATCGGGC‑ACATAATATCGATGGGCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCa                              <  1:361647/140‑1 (MQ=255)                                                                                               cgcgTACATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCa                              <  1:90172/140‑1 (MQ=255)                                                                                                    aCATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGAc                         <  1:496771/140‑1 (MQ=255)                                                                                                    aCATCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGAc                         <  2:845975/140‑1 (MQ=255)                                                                                                      aTCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCa                       <  2:634736/140‑1 (MQ=255)                                                                                                       tCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAg                      <  1:567388/140‑1 (MQ=255)                                                                                                       tCGGGC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAg                      <  2:726034/140‑1 (MQ=255)                                                                                                          ggC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAgatg                   >  2:824713/1‑140 (MQ=255)                                                                                                           gC‑AAATAATATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAgatga                  <  1:475829/140‑1 (MQ=255)                                                                                                               aataatATCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATca               <  1:824713/140‑1 (MQ=255)                                                                                                                 taataTCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATcaca             >  1:728531/1‑140 (MQ=255)                                                                                                                   atatCGGTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACt           <  1:437530/140‑1 (MQ=255)                                                                                                                         gTGGCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTg     >  2:727778/1‑140 (MQ=255)                                                                                                                            gCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCgg        >  2:213795/1‑134 (MQ=255)                                                                                                                            gCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCgg        <  1:213795/134‑1 (MQ=255)                                                                                                                            gCCGTAGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGAtt  >  1:77206/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                       AAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGC‑AAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATT  >  NC_000913/364697‑364958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑