Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,348,470	A→G	V92A (GTC→GCC)	dcuB ←	anaerobic C4‑dicarboxylate transporter DcuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,348,470	0	A	G	100.0%	256.9 / NA	74	V92A (GTC→GCC)	dcuB	anaerobic C4‑dicarboxylate transporter DcuB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/30);  total (44/30)

ATAGAACTTGCCGCCATCGGACGTTCCGGACGGATGTTGTTCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGACAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAACCAGCATGACATCAACTGGTG  >  NC_000913/4348335‑4348605                                                                                                                                        |                                                                                                                                        aTAGAACTTGCCGCCATCGGACGTTCCGGACGGATGTTGTTCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAAt                                                                                                                                     >  2:778760/1‑140 (MQ=255) aTAGAACTTGCCGCCATCGGACGTTCCGGACGGATGTTGTTCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAAt                                                                                                                                     >  1:2907/1‑140 (MQ=255)  tAGAACTTGCCGCCATCGGACGTTCCGGACGGATGTTGTTCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAAtt                                                                                                                                    <  1:436181/140‑1 (MQ=255)       cTTGCCGCCATCGGACGTTCCGTACTGATCTTGTTCTCAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGTGCGGCAATTGAGAc                                                                                                                               <  1:282151/140‑1 (MQ=255)         tGCCGCCATCGGACGTTCCGGACGGATGTTGTTCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACat                                                                                                                             >  1:18892/1‑140 (MQ=255)         tGCCGCCATCGGACGTTCCGGACGGATGTTGTTCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACat                                                                                                                             >  1:433227/1‑140 (MQ=255)         tGCCGCCATCGGACGTTCCGGACGGATGTTGTTCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGAAAAGAATGGTCAGTGTGCAGGTCACGAACGGTGCGGCAATTGAGACat                                                                                                                             >  2:282151/1‑140 (MQ=255)          gccgccATCGGACGTTCCGGACGGATGTTGTTCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCGGTGTACACGTCGCAAACGGCGAGGCAATTGAGACata                                                                                                                            >  1:267543/1‑140 (MQ=255)           ccgccATCGGACGTTCCGGACGGATGTTGTTCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGTGCGGCaa                                                                                                                                      <  2:138834/129‑1 (MQ=255)           ccgccATCGGACGTTCCGGACGGATGTTGTTCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGTGCGGCaa                                                                                                                                      >  1:138834/1‑129 (MQ=255)            cgccATCGGACGTTCCGGACGGATGTTGTTCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCGGTGGATATGTCACAAAGGGCGCTGCAATTGAGACATAtt                                                                                                                          >  2:378786/1‑140 (MQ=255)                    gACGTTCCGGACGGATGTTGTTCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTg                                                                                                                  <  2:2907/140‑1 (MQ=255)                     aCGTTCCGGACGGATGTTGTTCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAGGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTgc                                                                                                                 <  2:583598/140‑1 (MQ=255)                       gTTCCGGACGGATGTTGTTCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTgcgg                                                                                                               <  2:88181/140‑1 (MQ=255)                          ccGGACGGATGTTGTTCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGgcgc                                                                                                            <  1:519743/140‑1 (MQ=255)                                  aTGTTGTTCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCtt                                                                                                    >  1:198021/1‑140 (MQ=255)                                  aTGTTGTTCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGAGGCAAGTGAGACATATGTCGGATTGCGGCGGAGCAGCtt                                                                                                    >  2:389785/1‑140 (MQ=255)                                        ttCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGc                                                                                              >  1:714482/1‑140 (MQ=255)                                                    aCGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTgcagcaa                                                                                  >  2:430648/1‑139 (MQ=255)                                                    aCGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCAt                                                                                  <  2:433227/140‑1 (MQ=255)                                                    aCGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCAt                                                                                  <  2:488395/140‑1 (MQ=255)                                                    aCGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCAt                                                                                  <  2:565145/140‑1 (MQ=255)                                                              tgatCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGa                                                                        <  2:198021/140‑1 (MQ=255)                                                               gatCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGAc                                                                       >  1:646126/1‑140 (MQ=255)                                                                 tCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCg                                                                     >  1:695062/1‑140 (MQ=255)                                                                   ggCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGcc                                                                   >  2:213566/1‑140 (MQ=255)                                                                   agacagaTGGTGTAACCCACATCACTCGTCCCGCAAACAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGcc                                                                   <  1:220681/134‑1 (MQ=255)                                                                    gCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGccc                                                                  >  1:211132/1‑140 (MQ=255)                                                                      aGAATGGTGTAAACCGCATGACACGTACCCCAAAGAAGGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGa                                                                <  1:389785/140‑1 (MQ=255)                                                                      aGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGa                                                                <  2:102029/140‑1 (MQ=255)                                                                      aGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGa                                                                <  1:453387/140‑1 (MQ=255)                                                                      aGAATGGTATAAACCACATGACACGTACCGTCACGTATGTTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGa                                                                <  2:413909/140‑1 (MQ=255)                                                                               tAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCaa                                                       >  2:763123/1‑140 (MQ=255)                                                                                    cacaTGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTGGCACCATTACATCAAGGCCGCGCGAAGCTTGCactgtct                                                  >  2:220681/1‑134 (MQ=255)                                                                                     acaTGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGc                                                 <  2:211132/140‑1 (MQ=255)                                                                                       aTGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGTATGACATCAAGTCCGCCCGAATCTTGCAAGGTCGCCg                                               >  1:408399/1‑140 (MQ=255)                                                                                           cccGTACCGCAAAGAATGGTCAGTGCACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATgc                                           >  2:29386/1‑140 (MQ=255)                                                                                              gTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACAACAAGACCCCCCAAAGCTTGCAAGGTCGCCGATgccgc                                        >  1:287197/1‑140 (MQ=255)                                                                                               tACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATgccgcc                                       >  1:711595/1‑140 (MQ=255)                                                                                               tACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATgccgcc                                       >  2:103138/1‑140 (MQ=255)                                                                                                 ccGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGccac                                     >  2:620992/1‑140 (MQ=255)                                                                                                  cGCAAAGAATGGTCAGTTTACAGGTCACAAAAGGCCCGGAAATTGAGATACATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCAGGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGccacc                                    <  2:363895/140‑1 (MQ=255)                                                                                                   gCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGccacca                                   >  1:577861/1‑140 (MQ=255)                                                                                                    cAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATgcc                                          <  1:217820/132‑1 (MQ=255)                                                                                                    cAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATgcc                                          >  2:217820/1‑132 (MQ=255)                                                                                                    cAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCcaccac                                  >  2:89436/1‑140 (MQ=255)                                                                                                    cAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCcaccac                                  >  1:35878/1‑140 (MQ=255)                                                                                                    cAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGAGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCcaccac                                  >  2:501828/1‑140 (MQ=255)                                                                                                      aaGAATGGTCAGTGTACAGGTCCCAAACGGCGCGGAAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCg                                <  2:267543/140‑1 (MQ=255)                                                                                                      aaGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCg                                <  2:714482/140‑1 (MQ=255)                                                                                                      aaGAATGGTCAGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCg                                <  2:701633/140‑1 (MQ=255)                                                                                                                aGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAAc                      <  1:620992/140‑1 (MQ=255)                                                                                                                aGTGTACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAAc                      <  1:29386/140‑1 (MQ=255)                                                                                                                  tgtACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAACCa                    >  1:734157/1‑140 (MQ=255)                                                                                                                   gtACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAACTAg                   >  2:480288/1‑140 (MQ=255)                                                                                                                    tACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAACCAGc                  <  1:501828/140‑1 (MQ=255)                                                                                                                    tACAGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAACCAGc                  <  1:89436/140‑1 (MQ=255)                                                                                                                     cagggTCACAAACGGCGTGGCAATTGAGACATATTGCGGCTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAACCAGCa                 <  2:321603/137‑1 (MQ=255)                                                                                                                       aGGTCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAACCAGCATg               >  2:287296/1‑140 (MQ=255)                                                                                                                          tCACAAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAACCAGCATGACa            <  2:734157/140‑1 (MQ=255)                                                                                                                            acaAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAACCAGCATGACATc          >  1:518463/1‑140 (MQ=255)                                                                                                                            acaAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAACCAGCATGACATc          >  1:713410/1‑140 (MQ=255)                                                                                                                            acaAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAACCAGCATGACATc          >  2:643263/1‑140 (MQ=255)                                                                                                                            acaAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAACCAGCATGACATc          >  1:580285/1‑140 (MQ=255)                                                                                                                            acaAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTCCAAGGTCGGCGACGTCTCCACCACCTCAATGATAACCAGCATGACATc          >  2:376955/1‑140 (MQ=255)                                                                                                                            acaAACGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTACAAGGTCGACGAGGGCACCACCACCGCAATTATAACCAGAATGACATc          >  2:412144/1‑140 (MQ=255)                                                                                                                              aaaCGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCTGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGAAGCCGCCAGCACGGCAATGATAGCCAGCATGACATCaa        >  1:224434/1‑140 (MQ=255)                                                                                                                              aaaCGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAACCAGCATGACATCaa        >  2:81262/1‑140 (MQ=255)                                                                                                                              aaaCGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGAGGCCGCCAACACTGCTATGATAGCCAGCATGACATCaa        >  1:404279/1‑140 (MQ=255)                                                                                                                               aaCGGCGCGGCAATTCAGACATCTTTCGGCTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAACCAGCATGACATCAAc       <  1:412144/140‑1 (MQ=255)                                                                                                                                aCGGCGCGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAACCAGCATGACATCAACt      <  2:35878/140‑1 (MQ=255)                                                                                                                                aCGGCGCGGCAATTGAGACATACTTCGGGTTCCGGAGCACCAGCTTCTCGGCAATTTGCTGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAACCAGCATGACATCAACt      <  2:224434/140‑1 (MQ=255)                                                                                                                                   gcgcGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAACCAGCATGACATCAACtggt   >  2:150557/1‑140 (MQ=255)                                                                                                                                   gcgcGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGACACCACCGCAATGAAAACCAGAATGACATCAACtctg   >  1:321603/1‑137 (MQ=255)                                                                                                                                   gcgcGGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGGTGCCGATTCCGCCACTAGGGCAATGACAACCAGCATGACAGCAACtggt   >  2:413622/1‑140 (MQ=255)                                                                                                                                    cgcgGCAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCACCGATGCCACCATCACTGCAATGATCACCAACATGACATCAACtggtg  >  2:272238/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        ATAGAACTTGCCGCCATCGGACGTTCCGGACGGATGTTGTTCTTAATGGCGACGTCGTAGATGATCGGCAGAATGGTGTAAACCACATGACCCGTACCGCAAAGAATGGTCAGTGTACAGGTCACAAACGGCGCGACAATTGAGACATATTTCGGGTTGCGGCGCAGCAGCTTCTCGGCAATTTGCAGCATGACATCAAGACCGCCCGAAGCTTGCAAGGTCGCCGATGCCGCCACCACCGCAATGATAACCAGCATGACATCAACTGGTG  >  NC_000913/4348335‑4348605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑