Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	371,379	T→C	G47G (GGT→GGC)	mhpC →	2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	371,379	0	T	C	100.0%	132.9 / NA	39	G47G (GGT→GGC)	mhpC	2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/24);  total (15/24)

TTATCAGCCACAAACCGAAGCCGCCACCAGCCGTTTTCTGAATGTAGAAGAAGCGGGTAAAACGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGTGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAA  >  NC_000913/371244‑371513                                                                                                                                        |                                                                                                                                       ttATCAGCCACAAACCGAAGCCGCCACCAGCCGTTTTCTGAATGTAGAAGAAGCGGGTAAAACGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTa                                                                                                                                    >  1:783980/1‑140 (MQ=255)       gCCACAAACCGAAGCAGCCACCCGCCGTTATCTGAATCTAGAAGAAGCGGGTAAAACGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTActggct                                                                                                                              <  1:279681/140‑1 (MQ=255)                  aaGCCGCCACCAGCCGTTTTCTGAATGTAGAAGAAGCGGGTAAAACGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTc                                                                                                                   <  2:783980/140‑1 (MQ=255)                       gccACCAGCCGTTTTCTGAATGTAGAAGAAGCGGGTAAAACGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCg                                                                                                              <  1:151999/140‑1 (MQ=255)                                   tttCTGAATGTAGAAGAAGCGGGTAAAACGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATcc                                                                                                  <  2:812794/140‑1 (MQ=255)                                     tCTGAATGTAGAAGAAGCGGGTAAAACGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCTGCCCGGGCCCTAATGGGTGGGCGAAGTTCAGCCGCAATATCGATCCGc                                                                                                >  2:220136/1‑140 (MQ=255)                                         aaTGTAGAAGAAGCGGGTAAAACGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTTGCTGGGCCAACTTCAGCCGCAATATCGATCCGCTGGt                                                                                            >  1:288212/1‑140 (MQ=255)                                                     gCGGGTAAAACGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTa                                                                                >  2:764070/1‑140 (MQ=255)                                                             aaCGCTGCGCATCCATTTTAATTACTGCGGAAAAGTCGATGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGa                                                                        <  2:288212/140‑1 (MQ=255)                                                             aaCGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGa                                                                        <  1:764070/140‑1 (MQ=255)                                                                  tGCGCATCCATTTTAATGACTGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCctg                                                                   <  1:658432/140‑1 (MQ=255)                                                                            ttttAATGACTGCGGTCAAGGCTACCAAATCGTTGTCATGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTc                                                         <  1:220136/140‑1 (MQ=255)                                                                            ttttAATGACTGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTc                                                         >  1:135574/1‑140 (MQ=255)                                                                              ttAATGACTGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATTGATCCGCTGGTATAGGGGGGCTATCGGGGGGTCCTGCTGGATTGTCCg                                                       >  2:255745/1‑140 (MQ=255)                                                                              ttAATGACTGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCg                                                       >  1:501209/1‑140 (MQ=255)                                                                                      tGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGGGATCCTGCTGGATTGTCCGGGTTgggg                                               >  1:389/1‑140 (MQ=255)                                                                                      tGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCAGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGt                                                    <  1:90602/135‑1 (MQ=255)                                                                                      tGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCAGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGt                                                    >  2:90602/1‑135 (MQ=255)                                                                                       gCGGACAAGGCGACGCAACCGTCGTTCTGATGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGc                                              <  1:234115/140‑1 (MQ=255)                                                                                       gCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGTCTATCGGATGATCCAGCTGGACTGTCCGGGTTGGGGc                                              >  1:370753/1‑140 (MQ=255)                                                                                       gCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGc                                              >  2:661621/1‑140 (MQ=255)                                                                                        cGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCAGCTGGTAGAGGCGGGCTTTCGGGAGATTCTGTTGGTTTGGCCGGGGTGGGGCa                                             >  2:34290/1‑140 (MQ=255)                                                                                         ggACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCaa                                            <  2:446343/140‑1 (MQ=255)                                                                                                 cgacgaAACCGTTGTTCTGCTGCATCGTTCCGGCCCGTGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGAtt                                    <  1:255745/140‑1 (MQ=255)                                                                                                 cgacgaAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGAtt                                    <  1:661621/140‑1 (MQ=255)                                                                                                   acgaAACCGTTGTCCTGTTGCATGGTTCCGGTCCGGGCGCTACTGGCTTGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCg                                  <  2:284746/140‑1 (MQ=255)                                                                                                   acgaAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCg                                  <  2:135574/140‑1 (MQ=255)                                                                                                   acgaAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGAACCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCg                                  <  1:554540/140‑1 (MQ=255)                                                                                                   acgaAACCGTTGTCCTGCTGCAAGGTTCCGGACCGTGCGCTACTGGCTTGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCg                                  <  2:263466/140‑1 (MQ=255)                                                                                                    cgaAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCgg                                 <  2:389/140‑1 (MQ=255)                                                                                                    cgaAACCGTTGTCCTGCGGCATTGTTCCGGCTCGGCCGCCACTGGCTGAGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCgg                                 <  2:370753/140‑1 (MQ=255)                                                                                                     gaAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGt                                <  1:34290/140‑1 (MQ=255)                                                                                                      aaaCCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTc                               <  1:103795/140‑1 (MQ=255)                                                                                                       aaCCGTTGTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCg                              <  2:501209/140‑1 (MQ=255)                                                                                                              gTCCTGCTGCATGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAg                       >  2:200198/1‑140 (MQ=255)                                                                                                                         tGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGAt            <  2:594447/140‑1 (MQ=255)                                                                                                                         tGGTTCCGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGAt            >  1:594447/1‑140 (MQ=255)                                                                                                                              ccGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGAt       >  2:205169/1‑140 (MQ=255)                                                                                                                              ccGGCCCGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGAt       >  1:642848/1‑140 (MQ=255)                                                                                                                                   ccGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTaa  <  2:642848/140‑1 (MQ=255)                                                                                                                                   ccGGGCGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTaa  <  1:205169/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TTATCAGCCACAAACCGAAGCCGCCACCAGCCGTTTTCTGAATGTAGAAGAAGCGGGTAAAACGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGTGCTACTGGCTGGGCGAACTTCAGCCGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAA  >  NC_000913/371244‑371513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑