Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,384,173	C→T	T112I (ACA→ATA)	yjeM →	putative transporter YjeM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,384,173	0	C	T	100.0%	190.9 / NA	55	T112I (ACA→ATA)	yjeM	putative transporter YjeM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/23);  total (32/23)

AAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTCACCGTCGTTGCTTCTAAGGGGATTAATAAAATT  >  NC_000913/4384038‑4384306                                                                                                                                        |                                                                                                                                      aagaagGCGGTATCTTTTCCTGGATTAATAATAGTGTGGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTc                                                                                                                                   <  1:302810/140‑1 (MQ=255) aagaagGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTc                                                                                                                                   <  1:545532/140‑1 (MQ=255)           tATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTa                                                                                                                         >  1:82624/1‑140 (MQ=255)            aTCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAg                                                                                                                        <  1:137898/140‑1 (MQ=255)                     tGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGAc                                                                                                               >  1:736844/1‑140 (MQ=255)                      ggATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGAcc                                                                                                              <  1:558515/140‑1 (MQ=255)                          gAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTGCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGc                                                                                                          <  2:166850/140‑1 (MQ=255)                           aataatAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCa                                                                                                         >  1:587101/1‑140 (MQ=255)                                 agggtCGGACCACGTTTGGCCTTCATTGGTATGTTAATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTTCCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCg                                                                                                   <  2:353675/137‑1 (MQ=255)                                     tCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTAtt                                                                                               >  1:432172/1‑140 (MQ=255)                                     tCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTAtt                                                                                               >  1:502893/1‑140 (MQ=255)                                     tCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCATCTTTGGTAGCGCCATGCCCCAGCACTGGCGTAtt                                                                                               >  2:277275/1‑140 (MQ=255)                                      cGGACCACGTTTTGCCTTCATTTGTCCGTCTATGTGGCTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTg                                                                                              <  1:245124/140‑1 (MQ=255)                                        gACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGcc                                                                                            >  1:754977/1‑140 (MQ=255)                                             cGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATTATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACt                                                                                       <  1:748937/140‑1 (MQ=255)                                             cGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGATATGACCGAGCACTGGCGTATTGCCGGACt                                                                                       >  1:319374/1‑140 (MQ=255)                                             cGTTTTGCCTTCATTGGTACGTTTATGTGGTGTTCCTCTTATATCATCAGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACt                                                                                       <  2:299739/140‑1 (MQ=255)                                                   gCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGcc                                                                                 >  1:710529/1‑140 (MQ=255)                                                        cATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGc                                                                            >  1:476022/1‑140 (MQ=255)                                                              tACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAggtgg                                                                      >  1:60640/1‑140 (MQ=255)                                                                      tgtgGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTctg                                                              <  2:82624/140‑1 (MQ=255)                                                                           ttttCCTCTTATATCATTTGGATGGTGAGTACCTCCGCGCAAGTTTGGTTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTgctggc                                                         <  2:228944/140‑1 (MQ=255)                                                                            tttCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTgctggct                                                        >  2:14678/1‑140 (MQ=255)                                                                            tttCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTgctggct                                                        <  1:542666/140‑1 (MQ=255)                                                                              tCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCtgt                                                      <  2:464212/140‑1 (MQ=255)                                                                                 tctTATATCATCTGGATTGTGAGTACCTCCGTGAAAGTTGGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGc                                                   <  2:319374/140‑1 (MQ=255)                                                                                 tctTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGc                                                   <  2:587101/140‑1 (MQ=255)                                                                                    tataTCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATg                                                >  2:780093/1‑140 (MQ=255)                                                                                      tatCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGga                                              <  2:736844/140‑1 (MQ=255)                                                                                      tatCATCTGGATGGTCAGTACCGCCTCGAGAGTTTGGCTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGga                                              <  1:406993/140‑1 (MQ=255)                                                                                          atcTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGgatgat                                          >  2:770737/1‑140 (MQ=255)                                                                                             tGGATGGTGAGTACCTCTGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACAGGAGCCTAAGCAGGTGATTGGTCTGGTGGCTGTGGCATGGATGATTCt                                       >  2:224960/1‑140 (MQ=255)                                                                                             tGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCt                                       >  1:573123/1‑140 (MQ=255)                                                                                             tGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCt                                       >  1:473468/1‑140 (MQ=255)                                                                                             tGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACAGGAGCCTAAGCAGGTGGTTGGTCTGTTGGCGGTGGCATGGATGATTCt                                       >  2:338592/1‑140 (MQ=255)                                                                                               gATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTgg                                     >  1:532554/1‑140 (MQ=255)                                                                                               gATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTgg                                     >  2:497240/1‑140 (MQ=255)                                                                                                aTGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGATGGCTGTGGCATGGATGATTCTGGt                                    >  1:366930/1‑140 (MQ=255)                                                                                                   gTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTCAc                                 >  2:47051/1‑140 (MQ=255)                                                                                                    tGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTCAcc                                >  2:95411/1‑140 (MQ=255)                                                                                                    tGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTCAcc                                >  2:161011/1‑140 (MQ=255)                                                                                                    tGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGGGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTCAcc                                >  2:567485/1‑140 (MQ=255)                                                                                                      aGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTATGCAGGTGGGTGGCCTGCTGGCTGTGGCATGCATGATTCTGGTCACcgt                              >  2:280920/1‑140 (MQ=255)                                                                                                        tACCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGACGATTCTGGTCACcgtcg                            >  2:370214/1‑140 (MQ=255)                                                                                                         aCCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTCACcgtcgt                           <  2:722710/140‑1 (MQ=255)                                                                                                         aCCTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTCACcgtcgt                           <  1:780093/140‑1 (MQ=255)                                                                                                          ccTCCGCGAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTCACCGTCGtt                          <  1:14678/140‑1 (MQ=255)                                                                                                              cgcgAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGTATGGATGATTCTGGTCACCGTCGTTGCtt                      >  1:288902/1‑140 (MQ=255)                                                                                                              cgcgAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTCGCTGTGGCATGAATAATTCTGCTCACCGTCGTTGCtt                      >  1:422923/1‑140 (MQ=255)                                                                                                                cgAAAGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTCACCGTCGTTGCTTCt                    >  2:597044/1‑140 (MQ=255)                                                                                                                  aaaGTTTGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTCACCGTCGTTGCTTCTaa                  >  2:777701/1‑140 (MQ=255)                                                                                                                      tttGGGTACCGTTCTCTATATTCCTCTATGGTAACGACATGACCCAGCTCTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTCACCGTCGTTGCTTCTAAgggg              <  2:422923/140‑1 (MQ=255)                                                                                                                      tttGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTCACCGTCGTTGCTTCTAAgggg              <  2:60640/140‑1 (MQ=255)                                                                                                                        tGGGTACCGTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTCACCGTCGTTGCTTCTAAGGGGAt            <  2:187499/140‑1 (MQ=255)                                                                                                                                gTTCTCAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTCACCGTCGTTGCTTCTAAGGGGATTAATaaaa    >  2:217269/1‑140 (MQ=255)                                                                                                                                  tctcAATATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTCACCGTCGTTGCTTCTAAGGGGATTAATAAAAtt  <  1:370214/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      AAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTCACCGTCGTTGCTTCTAAGGGGATTAATAAAATT  >  NC_000913/4384038‑4384306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑