Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,403,092	T→C	V352A (GTT→GCT)	hflK →	regulator of FtsH protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,403,092	0	T	C	100.0%	161.7 / NA	48	V352A (GTT→GCT)	hflK	regulator of FtsH protease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/20);  total (28/20)

CTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGT  >  NC_000913/4402958‑4403228                                                                                                                                       |                                                                                                                                         cTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGc                                                                                                                                     >  1:658224/1‑140 (MQ=255)           cTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACCATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCa                                                                                                                           >  1:344930/1‑140 (MQ=255)            ttCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAg                                                                                                                          <  1:577028/140‑1 (MQ=255)            ttCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAg                                                                                                                          <  2:658224/140‑1 (MQ=255)               tGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATg                                                                                                                       >  1:6853/1‑140 (MQ=255)                  cGGAATATAAAGCCGCGCCGGAAATTACTCGGGAGTGTCCGTATATCGTGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTg                                                                                                                    <  2:344930/140‑1 (MQ=255)                            aGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTa                                                                                                          >  1:6036/1‑140 (MQ=255)                               cgcgCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACg                                                                                                       >  1:742104/1‑140 (MQ=255)                                gcgcCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGc                                                                                                      >  2:108657/1‑140 (MQ=255)                                 cgcCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGcc                                                                                                     >  2:864692/1‑140 (MQ=255)                                 cgcCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGcc                                                                                                     >  1:430338/1‑140 (MQ=255)                                 cgcCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGcc                                                                                                     >  2:817911/1‑140 (MQ=255)                                 cgcCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGAAACCTGATGGCTGTGCCGTTATACCCGATGTTGAAAGGTGGTAACGcc                                                                                                     >  2:294034/1‑140 (MQ=255)                                       aaaTTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCGCTgcg                                                                                               <  2:6853/140‑1 (MQ=255)                                          ttACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTgcggcg                                                                                            <  1:108657/140‑1 (MQ=255)                                             cTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAag                                                                                         <  2:6036/140‑1 (MQ=255)                                               cgcgAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAagag                                                                                       <  1:459853/140‑1 (MQ=255)                                                 cgAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCg                                                                                     <  2:742104/140‑1 (MQ=255)                                                           tataTCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGc                                                                           <  2:758305/140‑1 (MQ=255)                                                                  agaCGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAAt                                                                    >  2:124433/1‑140 (MQ=255)                                                                  agaCGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAAt                                                                    >  1:775091/1‑140 (MQ=255)                                                                   gaCGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGTGGCAAATAGCGATTACGGTGCCAGCAATc                                                                   >  1:388108/1‑140 (MQ=255)                                                                     cGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAATGGTGGTAACGCCGCTGGGGCGAAGAGCGATAACGGTGCCAGCAATctg                                                                 >  2:315527/1‑140 (MQ=255)                                                                     cGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAACGGTGGTAATGCCACTGTGGCGAAGACCGATGACGGTGCCAGCAATctg                                                                 >  2:280378/1‑140 (MQ=255)                                                                     cGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATctg                                                                 >  2:586729/1‑140 (MQ=255)                                                                     cGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATctg                                                                 >  2:496726/1‑140 (MQ=255)                                                                                         aaCACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCctc                                             <  1:864692/140‑1 (MQ=255)                                                                                           cacCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTTATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCtt                                           <  2:411599/140‑1 (MQ=255)                                                                                              ccGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTtcct                                        <  1:124433/140‑1 (MQ=255)                                                                                               cGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTtcctc                                       >  2:787536/1‑140 (MQ=255)                                                                                                     gTGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAAc                                 <  1:817911/140‑1 (MQ=255)                                                                                                      tGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAAcc                                >  2:820628/1‑140 (MQ=255)                                                                                                      tGCTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAAcc                                >  1:797007/1‑140 (MQ=255)                                                                                                        cTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATGTGCTGCGACTGGCGACAGCCTCTTCCTCCACAACCAg                              >  1:233936/1‑140 (MQ=255)                                                                                                        cTGGTTAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAg                              >  2:76671/1‑140 (MQ=255)                                                                                                            ttAACGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGa                          <  1:787536/140‑1 (MQ=255)                                                                                                              aaCGATAAGGGTGGCAAGCTGAAGGCTCTGATGATCGACCAGATGCTGTAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGagc                        <  2:416401/140‑1 (MQ=255)                                                                                                              aaCGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGagc                        <  2:775091/140‑1 (MQ=255)                                                                                                                cGATAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGagcaa                      >  1:629430/1‑140 (MQ=255)                                                                                                                  aTAAAGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGagcaagc                    >  2:211336/1‑140 (MQ=255)                                                                                                                     aaGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAac                 >  1:46464/1‑140 (MQ=255)                                                                                                                     aaGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAac                 >  2:475586/1‑140 (MQ=255)                                                                                                                     aaGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAac                 >  2:769919/1‑140 (MQ=255)                                                                                                                      aGGTGGCAACCTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCCCCAGCCTTTTCATCGACAACCAGTGGAGCAAGCAaca                >  1:416401/1‑140 (MQ=255)                                                                                                                               ccTGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTcca       <  1:211336/140‑1 (MQ=255)                                                                                                                                 tGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTccacc     <  1:769919/140‑1 (MQ=255)                                                                                                                                 tGATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTccacc     <  1:76671/140‑1 (MQ=255)                                                                                                                                  gATGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTACCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTccacca    >  1:52573/1‑140 (MQ=255)                                                                                                                                    tGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGt  <  2:758497/140‑1 (MQ=255)                                                                                                                                    tGGCTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGt  <  2:797007/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         CTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGT  >  NC_000913/4402958‑4403228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑