| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,446,347 | A→G | T746A (ACC→GCC) | tamB → | translocation and assembly module subunit TamB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,446,347 | 0 | A | G | 97.7% | 152.7 / NA | 44 | T746A (ACC→GCC) | tamB | translocation and assembly module subunit TamB |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (20/23); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGGGCCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGC > NC_000913/4446213‑4446482 | tCGGGCCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACt < 2:131760/140‑1 (MQ=255)tCGGGCCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACt < 2:567887/140‑1 (MQ=255)tCGGGCCACACTGCTGGCTTAACCCAAATGCGGAACTTTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACt < 1:330123/140‑1 (MQ=255) aaCCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCAc < 1:660880/140‑1 (MQ=255) aCCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACg > 2:661660/1‑140 (MQ=255) aCCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGACACTCAGGCCAGCGGCATCTTCACg > 2:584911/1‑140 (MQ=255) aaTGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGTGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGACCAGCGGTATCTTCACGGGTaa > 2:338757/1‑140 (MQ=255) aaTGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTaa > 2:636309/1‑140 (MQ=255) tGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAg < 2:557179/140‑1 (MQ=255) aCTGTGCGTGCCGCAAACTATCCATGCGGGGCCCGCAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACg < 2:259098/140‑1 (MQ=255) gtgcgtgcCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTg > 2:586169/1‑140 (MQ=255) tgcgtgcCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGc < 2:202160/131‑1 (MQ=255) tgcgtgcCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGc > 1:202160/1‑131 (MQ=255) tgcgtgcCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGc > 2:652782/1‑140 (MQ=255) ccGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGa < 2:537473/140‑1 (MQ=255) aaaCTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGa < 2:130001/136‑1 (MQ=255) aaaCTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGa > 1:130001/1‑136 (MQ=255) aTCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGaa < 1:584911/140‑1 (MQ=255) aTCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGaa < 1:661660/140‑1 (MQ=255) cGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGCACGATGCCTGGGAAACCACGAAAGAgggg > 1:352842/1‑140 (MQ=255) cGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGAACGTTGTCTGGGACACCACGAAAGAgggg > 1:324816/1‑140 (MQ=255) gggggCCGAAGGGCGTGAGGTGTTGAATCTCAACCGCTTTGACCTCGCTATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGc < 2:352842/140‑1 (MQ=255) gggggCCGAAGGGCGTGAGGTGTTGAATCTCAACCACTTCGACCTCGCTATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGc < 2:324816/140‑1 (MQ=255) ggggCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCt > 2:449730/1‑140 (MQ=255) ggggCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCt > 2:432040/1‑140 (MQ=255) gggCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTg < 1:652782/140‑1 (MQ=255) ggCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGc > 1:120038/1‑140 (MQ=255) ccGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCg < 1:636309/140‑1 (MQ=255) ccGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCg < 1:449730/140‑1 (MQ=255) ccGAAGGGCGTGCGGTGGTGAATCGCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCg < 2:703350/140‑1 (MQ=255) aaGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAg < 2:120038/140‑1 (MQ=255) gTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGt > 1:197628/1‑140 (MQ=255) tGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGGTGCCGCAGGGCAGTa > 1:355445/1‑140 (MQ=255) tGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTa > 1:131452/1‑140 (MQ=255) gCGGTGGTGAATCTCAAGCGCTTCGACCTCGCCATGCTGAAACAGTTTCTGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTAt < 2:328566/140‑1 (MQ=255) gCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTAt < 2:131452/140‑1 (MQ=255) gCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTAt < 1:586169/140‑1 (MQ=255) gCGGTGGTGAATCTCAAACGCTTCGACCTCGCCATCCTGTAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTAt < 2:355445/140‑1 (MQ=255) ggtgAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCAccc > 2:134989/1‑140 (MQ=255) gAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCttt < 1:134989/140‑1 (MQ=255) aCCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGt < 2:197628/140‑1 (MQ=255) tGAAACCGTTTATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAAcc > 1:48034/1‑140 (MQ=255) aCCGTTTATGCCAGAAGACACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCa > 1:667602/1‑140 (MQ=255) tttATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGa > 1:125388/1‑140 (MQ=255) tATGCCAGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATg > 2:74992/1‑140 (MQ=255) aGAAGCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGgcgc < 2:48034/140‑1 (MQ=255) | TCGGGCCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGC > NC_000913/4446213‑4446482 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |