Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,467,292	A→G	intergenic (‑46/‑139)	treR ← / → mgtL	DNA‑binding transcriptional repressor TreR/leader peptide MgtL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,467,292	0	A	G	100.0%	190.8 / NA	55	intergenic (‑46/‑139)	treR/mgtL	DNA‑binding transcriptional repressor TreR/leader peptide MgtL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/34);  total (21/34)

CGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGAATATTATTGGATTCTCCTTATTATTTGCGGCGCTTTTTTCACTTACCGGA  >  NC_000913/4467156‑4467424                                                                                                                                         |                                                                                                                                     cgccgcTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATACTGTTGTCCTGTTACGTGTTGTTTAATTATTTGAGCCTAACGTTGccc                                                                                                                                   >  2:358035/1‑140 (MQ=255)   ccgcTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGt                                                                                                                                 <  2:685231/140‑1 (MQ=255)      cTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCa                                                                                                                              <  2:775926/140‑1 (MQ=255)             tATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCTGTGCATTCAGCa                                                                                                                       <  2:182353/140‑1 (MQ=255)              aTTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCACGTGCATTCAGCaa                                                                                                                      <  2:612779/140‑1 (MQ=255)                       ccGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAg                                                                                                             >  1:799132/1‑140 (MQ=255)                                tGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGGGCATTCCGCAATGTGTAAAGTCTGGTTTa                                                                                                    >  1:384287/1‑140 (MQ=255)                                 gTAGATTTCCCCACGCCTCTTATGCGCGCGACATCCTTGTTGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTAt                                                                                                   <  2:243040/140‑1 (MQ=255)                                   aGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGt                                                                                                       >  1:425462/1‑134 (MQ=255)                                   aGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGt                                                                                                       <  2:425462/134‑1 (MQ=255)                                         ccccACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGtt                                                                                           <  2:840844/140‑1 (MQ=255)                                          cccACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCTATGAGTTAAGTCTAGTTTATCGTTGGttt                                                                                          >  1:243040/1‑140 (MQ=255)                                          cccACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGttt                                                                                          >  1:166246/1‑140 (MQ=255)                                            cACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAg                                                                                        >  1:22160/1‑140 (MQ=255)                                            cACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAg                                                                                        >  1:686092/1‑140 (MQ=255)                                            cACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGGAATGTCTGGTTTATCGTTGGTTTAg                                                                                        >  1:419700/1‑140 (MQ=255)                                              cgccgcTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGtt                                                                                      >  1:127220/1‑140 (MQ=255)                                               gccgcTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTg                                                                                     <  2:686092/140‑1 (MQ=255)                                                  gcTTAAGCCCGCGATAACTTTGATGGTCAGCCGCTCTTGAATCCTGTTATCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTTGTTTAGTTGTca                                                                                  <  2:419700/140‑1 (MQ=255)                                                   cTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTcag                                                                                 <  2:799132/140‑1 (MQ=255)                                                    ttAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTcagc                                                                                <  1:648857/140‑1 (MQ=255)                                                    ttAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTcagc                                                                                <  2:166246/140‑1 (MQ=255)                                                    ttAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAACAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTcagc                                                                                <  1:506829/140‑1 (MQ=255)                                                           cgcgATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTGAAGGCTGATTTATCGTTGGTTAAGTTGTCAGCAGGtatt                                                                         >  2:240170/1‑140 (MQ=255)                                                           cgcgATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTcagc                                                                                <  1:522187/133‑1 (MQ=255)                                                           cgcgATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTcagc                                                                                >  2:522187/1‑133 (MQ=255)                                                             cgATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGtattat                                                                       >  1:88974/1‑140 (MQ=255)                                                              gATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATtata                                                                      <  2:213105/140‑1 (MQ=255)                                                              gATATCTTTGATGGTCAGCCGATTTTGCACCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATtata                                                                      <  1:240170/140‑1 (MQ=255)                                                               atatCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTatat                                                                     <  2:134650/140‑1 (MQ=255)                                                                      tGATGGTCAGCCGATTTTGCATGCTGTTGTCGTATTACGTGTTGTTTAGTTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATa                                                              <  2:384287/140‑1 (MQ=255)                                                                        aTGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGa                                                            >  1:508830/1‑140 (MQ=255)                                                                            ttaGCCGCTTTTGCATCCTGTTGTCCTTTATCGTCTTGTTTAAATATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCt                                                        <  1:534936/138‑1 (MQ=255)                                                                            tCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCt                                                        >  2:45310/1‑140 (MQ=255)                                                                            tCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCt                                                        >  1:777250/1‑140 (MQ=255)                                                                            tCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCt                                                        <  2:199545/140‑1 (MQ=255)                                                                            tCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCt                                                        <  1:45310/140‑1 (MQ=255)                                                                              aGCCGATTTTGCATCATGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTAc                                                      <  1:73469/140‑1 (MQ=255)                                                                                 cGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGaa                                                   <  2:508830/140‑1 (MQ=255)                                                                                    ttttGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGAatat                                                <  2:88974/140‑1 (MQ=255)                                                                                        gCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGAATattatt                                            <  1:156779/140‑1 (MQ=255)                                                                                            ccTGTTGTTCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGAATATTATTGGAt                                        <  2:127220/140‑1 (MQ=255)                                                                                             cTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGaa                                                   <  1:832527/128‑1 (MQ=255)                                                                                             cTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGaa                                                   <  1:554293/128‑1 (MQ=255)                                                                                             cTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGaa                                                   >  2:832527/1‑128 (MQ=255)                                                                                             cTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGaa                                                   >  2:554293/1‑128 (MQ=255)                                                                                              tgttgtCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGAATATTATTGGATtc                                      <  1:123100/140‑1 (MQ=255)                                                                                              tgttgtCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGAATATTATTGGATtc                                      >  1:22683/1‑140 (MQ=255)                                                                                                   tCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGAAtatta                                              <  2:670808/127‑1 (MQ=255)                                                                                                   tCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGAAtatta                                              >  1:670808/1‑127 (MQ=255)                                                                                                             tgttgtTTAATTATTTGAGCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGAATATTATTGGATTCTCCTTATTATTTgcg                       >  1:102856/1‑140 (MQ=255)                                                                                                                               gCCTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGAATATTATTGGATTCTCCTTATTATTTGCGGCGCTTTTTTCACTTAcc     <  1:358035/140‑1 (MQ=255)                                                                                                                                ccTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGAATATTATTGGATTCTCCTTATTATTTGCGGCGCTTTTTTCACTTACCg    <  2:777250/140‑1 (MQ=255)                                                                                                                                 cTAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGAATATTATTGGATTCTCCTTATTATTTGCGGCGCTTTTTTCACTTACCgg   <  2:102856/140‑1 (MQ=255)                                                                                                                                  tAACGTTGCCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGAATATTATTGGATTCTCCTTATTATTTGCGGCGCTTTTTTCACTTACCGGa  <  2:22683/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTTTAGTTGTCAGCAGGTATTATATCGCCATAGATGCTACGAATATTATTGGATTCTCCTTATTATTTGCGGCGCTTTTTTCACTTACCGGA  >  NC_000913/4467156‑4467424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑