Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	70,424	A→G	Y13C (TAC→TGC)	araC →	DNA‑binding transcriptional dual regulator AraC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	70,424	0	A	G	100.0%	217.0 / NA	64	Y13C (TAC→TGC)	araC	DNA‑binding transcriptional dual regulator AraC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/29);  total (35/29)

TGGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGA  >  NC_000913/70288‑70558                                                                                                                                         |                                                                                                                                       tGGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTc                                                                                                                                     >  1:754944/1‑140 (MQ=255)   ggTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGTAGCGGAAAATGAGCCCCTACTGCCGGAATGCTCGt                                                                                                                                   >  2:420843/1‑140 (MQ=255)       aGCGAGAAGAGCCAGTACAAGATGCAGTGACCGCAATGTTTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTaa                                                                                                                               <  2:315084/140‑1 (MQ=255)         cGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGtt                                                                                                                                  <  2:838311/135‑1 (MQ=255)         cGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGtt                                                                                                                                  >  1:838311/1‑135 (MQ=255)               gagCCAGTAAAAGACCCAGTGAGGGGAATTTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATc                                                                                                                       <  1:420843/140‑1 (MQ=255)                  ccAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCtgg                                                                                                                    >  2:139634/1‑140 (MQ=255)                  ccAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCtgg                                                                                                                    >  2:506915/1‑140 (MQ=255)                            aCGCAGTGACGGCAATGTCTGGTGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGttt                                                                                                          >  2:26028/1‑140 (MQ=255)                                aGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACg                                                                                                      >  2:745942/1‑140 (MQ=255)                                aGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACg                                                                                                      >  1:726060/1‑140 (MQ=255)                                            gTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGcc                                                                                          <  1:26028/140‑1 (MQ=255)                                            gTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGcc                                                                                          <  2:754944/140‑1 (MQ=255)                                            gTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGcc                                                                                          >  2:76162/1‑140 (MQ=255)                                                      atatGGACAATTGGTTTCTTCTCTGAATGGTGGGAGAATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGTGGCCAACGGTTAtc                                                                                >  2:116523/1‑140 (MQ=255)                                                       tatGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAtct                                                                               >  2:186736/1‑140 (MQ=255)                                                         tGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCg                                                                             >  2:695043/1‑140 (MQ=255)                                                         tGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCg                                                                             >  2:101807/1‑140 (MQ=255)                                                                ttGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtttttt                                                                      >  1:772759/1‑140 (MQ=255)                                                                ttGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtttttt                                                                      >  1:47298/1‑140 (MQ=255)                                                                ttGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtttttt                                                                      >  1:173317/1‑140 (MQ=255)                                                                ttGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCCGGTGGCGGTTTTTACGGCGATTGAGACCAAGGGTTATCTCGAtttttt                                                                      >  2:252221/1‑140 (MQ=255)                                                                 tGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTGAACGCCGCTTGAGGCCAACGGCTATCTCGAttttttt                                                                     >  1:337413/1‑140 (MQ=255)                                                                   gTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTAt                                                                   <  1:139634/140‑1 (MQ=255)                                                                     ttcttcTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGa                                                                            >  2:814385/1‑129 (MQ=255)                                                                     ttcttcTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGa                                                                            <  1:814385/129‑1 (MQ=255)                                                                     ttcttcTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcg                                                                 >  2:797168/1‑140 (MQ=255)                                                                       cttctCTGAATGGTGGGCGTATGAAAAGTATAGCTGAAGAGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgac                                                               <  2:317388/140‑1 (MQ=255)                                                                          ctctGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccga                                                            <  2:797334/140‑1 (MQ=255)                                                                          ctctGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccga                                                            >  2:429604/1‑140 (MQ=255)                                                                          ctctGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccga                                                            >  1:549720/1‑140 (MQ=255)                                                                             tGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGaccgaccg                                                         <  1:76162/140‑1 (MQ=255)                                                                             tGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACCGTTATCCCGATTTATTTATCGaccgaccg                                                         >  1:317388/1‑140 (MQ=255)                                                                               aaTGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCt                                                       <  1:745942/140‑1 (MQ=255)                                                                               aaTGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCt                                                       <  1:429604/140‑1 (MQ=255)                                                                                  ggtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTggg                                                    >  2:551215/1‑140 (MQ=255)                                                                                   gtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGa                                                   <  2:772759/140‑1 (MQ=255)                                                                                    tggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGCTTGAGGCCAACGCGTATCTCGATTTTTTTAACGACCGACCGCTGGGaa                                                  >  2:342856/1‑140 (MQ=255)                                                                                    tggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCGACGGTTAACTCGATTTCTTTATCGACCGACCGCTGGGaa                                                  >  2:322443/1‑140 (MQ=255)                                                                                    tggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGaa                                                  >  2:112673/1‑140 (MQ=255)                                                                                    tggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGaa                                                  <  2:549720/140‑1 (MQ=255)                                                                                        aGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGaa                                              <  2:726060/140‑1 (MQ=255)                                                                                        aGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGaa                                              <  1:101807/140‑1 (MQ=255)                                                                                        aGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGaa                                              <  2:173317/140‑1 (MQ=255)                                                                                        aGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGaa                                              <  1:695043/140‑1 (MQ=255)                                                                                        aGTATGAAAAGTATGGCGGAAGGGCAAAATGCTCCACTGCTGCCGGGATGCTCGTTTAACGCTCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGaa                                              <  2:337413/140‑1 (MQ=255)                                                                                        aGTATGAAAAGTATGCCTGAAGAGCCAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGaa                                              <  1:252221/140‑1 (MQ=255)                                                                                         gTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGATATTTCGCTTTTTTTATCGACCGACCGCTGGGAATGaaa                                             >  2:291100/1‑140 (MQ=255)                                                                                             gAAAAGTATGGCTGAAGCGCAAGATCATCACCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGtt                                         <  1:322443/140‑1 (MQ=255)                                                                                              aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTta                                        >  2:839404/1‑140 (MQ=255)                                                                                               aaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTtat                                       <  1:641316/140‑1 (MQ=255)                                                                                                  gTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATAtt                                    >  1:76684/1‑140 (MQ=255)                                                                                                      ggCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCa                                >  2:608744/1‑140 (MQ=255)                                                                                                             gcgcAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCAc                         <  1:797168/140‑1 (MQ=255)                                                                                                             gcgcAAAATGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCAc                         >  1:61611/1‑140 (MQ=255)                                                                                                                 aaaaTGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTGATCGACCGACCGCTGGAAATGAAAGGGTATATTCTCAATCTCACCAtt                     >  2:356831/1‑140 (MQ=255)                                                                                                                   aaTGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTcg                   <  1:112673/140‑1 (MQ=255)                                                                                                                   aaTGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTcg                   <  1:839404/140‑1 (MQ=255)                                                                                                                   aaTGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTcg                   <  1:608744/140‑1 (MQ=255)                                                                                                                   aaTGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTcg                   <  1:291100/140‑1 (MQ=255)                                                                                                                   aaTGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTcg                   <  1:163388/140‑1 (MQ=255)                                                                                                                   aaTGATCCCCTGCTGCCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTcg                   <  1:116523/140‑1 (MQ=255)                                                                                                                   aaTGATCCCCTGCTGACGGGATGCTCGTTAAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAATTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTTACCATTcg                   <  1:342856/140‑1 (MQ=255)                                                                                                                               ctgcCGGGATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGggt       >  1:864058/1‑140 (MQ=255)                                                                                                                                    gggATGCTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGa  >  1:58461/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TGGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGA  >  NC_000913/70288‑70558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑