Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	379,940	A→G	intergenic (‑59/+129)	frmR ← / ← yaiO	DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	379,940	0	A	G	100.0%	165.1 / NA	49	intergenic (‑59/+129)	frmR/yaiO	DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/26);  total (23/26)

GTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTC  >  NC_000913/379803‑380078                                                                                                                                          |                                                                                                                                           gTTCCGGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTa                                                                                                                                          <  2:593848/140‑1 (MQ=255) gTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTATATATGTCAATGCGTa                                                                                                                                          <  2:68776/140‑1 (MQ=255)  ttCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTAc                                                                                                                                         <  1:629883/140‑1 (MQ=255)  ttCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTAc                                                                                                                                         >  1:599560/1‑140 (MQ=255)   tCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTAcc                                                                                                                                        >  2:682560/1‑140 (MQ=255)    ccAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTAccc                                                                                                                                       <  2:92748/140‑1 (MQ=255)      agagCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTAccccc                                                                                                                                     <  2:599560/140‑1 (MQ=255)      agagCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTAccccc                                                                                                                                     <  2:489366/140‑1 (MQ=255)      agagCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTAccccc                                                                                                                                     <  1:715666/140‑1 (MQ=255)        agCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCt                                                                                                                                   <  1:74477/140‑1 (MQ=255)         gCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCta                                                                                                                                  <  2:698118/140‑1 (MQ=255)         gCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCta                                                                                                                                  <  1:533653/140‑1 (MQ=255)         gCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCta                                                                                                                                  <  2:700427/140‑1 (MQ=255)         gCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCta                                                                                                                                  <  1:861529/140‑1 (MQ=255)         gCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCta                                                                                                                                  <  1:37269/140‑1 (MQ=255)         gCATCAATCTGCCCCCGAATACCACCAACCCGAGTAAAGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCta                                                                                                                                  <  1:308814/140‑1 (MQ=255)           aTCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCtata                                                                                                                                <  2:614107/140‑1 (MQ=255)           aTCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCtata                                                                                                                                <  2:432566/140‑1 (MQ=255)             cAATCTGCCCCCGAATACGACGAGCTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTAccccc                                                                                                                                     <  2:79529/133‑1 (MQ=255)             cAATCTGCCCCCGAATACGACGAGCTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTAccccc                                                                                                                                     >  1:79529/1‑133 (MQ=255)             cAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTAc                                                                                                                                         <  2:672382/129‑1 (MQ=255)             cAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTAc                                                                                                                                         >  1:672382/1‑129 (MQ=255)             cAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCtata                                                                                                                                >  1:458105/1‑138 (MQ=255)             cAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCtata                                                                                                                                <  2:458105/138‑1 (MQ=255)              aaTCTGCCCCCGAATGCGACGAACTAGAGGAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCTATAGta                                                                                                                             <  1:370261/140‑1 (MQ=255)               aTCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTTGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCTATAGtat                                                                                                                            <  2:841547/140‑1 (MQ=255)                    cccccGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGCACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTTTAGGGGGGCATTCTATCTGTCAATGTGTACTCCCCTATAGTATATAac                                                                                                                       >  2:370261/1‑140 (MQ=255)                                                    ttttCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTgg                                                                                       >  2:153710/1‑140 (MQ=255)                                                                ccGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACaaa                                                                           <  1:682560/140‑1 (MQ=255)                                                                ccGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACaaa                                                                           <  1:153710/140‑1 (MQ=255)                                                                 cGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAag                                                                          <  1:615534/140‑1 (MQ=255)                                                                           ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCTATAGTATATAACACTGGATAATAAAATATATCCGGGGAATGTGGTCGGAGAACAAAGAGGAAAgggg                                                                >  2:364860/1‑140 (MQ=255)                                                                           ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggg                                                                >  1:100238/1‑140 (MQ=255)                                                                           ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggg                                                                >  1:431896/1‑140 (MQ=255)                                                                                ttCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggc                                                           >  1:713696/1‑139 (MQ=255)                                                                                                            aCTATAGGGGGGTATTCTATATGTCAATGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa                             >  1:501314/1‑140 (MQ=255)                                                                                                                taGGGGGGTATTCTATATGTCAATGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAGGAGGAAA‑‑GTGGGTGGCGAATCGGCAAGGAAGGCCGCCCCGGATAgcgg                         >  2:375554/1‑140 (MQ=255)                                                                                                                   gggggTATTCTATATGTCAATGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGTATAgcgggcg                      >  2:746116/1‑140 (MQ=255)                                                                                                                   gggggTATTCTATATGTCAATGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcg                      >  2:761873/1‑140 (MQ=255)                                                                                                                     gggTATTCTATATGTCAATGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                    >  1:206422/1‑140 (MQ=255)                                                                                                                     gggTATTCTATATGTCAATGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGCGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAACGggcggc                    >  1:151533/1‑140 (MQ=255)                                                                                                                        tATTCTATATGTCAATGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑‑TAATCGGCAGGGGAGGCCGCCCCGGGTAGCGGGGGGgaga                 >  1:99738/1‑140 (MQ=255)                                                                                                                        tATTCTATATGTCAATGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGa                 <  2:100238/140‑1 (MQ=255)                                                                                                                        tATTCTATATGTCAATGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGa                 >  1:466236/1‑140 (MQ=255)                                                                                                                             tataTGTCAATGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg                         <  2:129499/127‑1 (MQ=255)                                                                                                                             tataTGTCAATGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg                         >  1:129499/1‑127 (MQ=255)                                                                                                                                     aaTGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGCCCGCCCCAGATAGCTGGCGGCTGAAGGAATCAGAAtt    >  1:405264/1‑140 (MQ=255)                                                                                                                                       tGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTc  >  2:723509/1‑140 (MQ=255)                                                                                                                                       tGCGTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑GGGGGGGTGCTAACCGGCAGGGAAGACCGTCTCCAATAGCGGACGGCCGAAGGAATCAGAATTTc  >  2:407250/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                           GTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTC  >  NC_000913/379803‑380078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑