Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,516,073	A→G	V202A (GTC→GCC)	fecA ←	ferric citrate outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,516,073	0	A	G	100.0%	129.9 / NA	40	V202A (GTC→GCC)	fecA	ferric citrate outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/17);  total (23/17)

TTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAG  >  NC_000913/4515941‑4516209                                                                                                                                     |                                                                                                                                         ttCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGAt                                                                                                                                   >  1:89518/1‑140 (MQ=255)  tCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCCGACAGTAAGGGGCTAAGGGGGCGGGGATg                                                                                                                                  >  1:369706/1‑140 (MQ=255)           cgccCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGGTGAACTTTAGGGGCGATGGGGGAGGGGATGCCGTCCATc                                                                                                                         >  2:379709/1‑140 (MQ=255)               cACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGa                                                                                                                     <  1:631147/140‑1 (MQ=255)                     ctGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCg                                                                                                               <  2:89518/140‑1 (MQ=255)                                     accgcaccACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGgcg                                                                                               >  2:521281/1‑140 (MQ=255)                                     accgcaccACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGgcg                                                                                               <  1:521281/140‑1 (MQ=255)                                       cgcaccACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGcgcg                                                                                             >  2:818885/1‑140 (MQ=255)                                         caccaccacCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCggg                                                                                           >  2:796772/1‑140 (MQ=255)                                            caccaccGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTc                                                                                        >  2:660442/1‑140 (MQ=255)                                              ccaccGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAg                                                                                      >  2:444015/1‑140 (MQ=255)                                              ccaccGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACAGTCTAGCGGCTGGAGAGGCGCGGGTTCAg                                                                                      >  1:398864/1‑140 (MQ=255)                                                 ccGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGcc                                                                                   >  1:442294/1‑140 (MQ=255)                                                    cgTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCg                                                                                >  2:446065/1‑140 (MQ=255)                                                            gTCAATGGCATCCATGTGGCCGTGCGAAACGCGCGCCAGAGAAAGCTGTGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCaa                                                                        <  2:398864/140‑1 (MQ=255)                                                                aTGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGtt                                                                    <  1:446065/140‑1 (MQ=255)                                                                aTGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGAAGGTCGAGCAGCTGGCGGGGCGCGGGGTCAGGCCCCGGATGCCAAAGtt                                                                    >  2:269906/1‑140 (MQ=255)                                                                 tgacATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTc                                                                   >  2:728615/4‑140 (MQ=255)                                                                 tGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGGTCAGGCCCCGGATGCCAAAGTTc                                                                   >  1:443443/1‑140 (MQ=255)                                                                      tCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGc                                                              >  1:32898/1‑140 (MQ=255)                                                                      tCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGc                                                              <  2:32898/140‑1 (MQ=255)                                                                       ccATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGcc                                                             >  2:670660/1‑140 (MQ=255)                                                                                  aGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGcc                                                  >  2:835624/1‑139 (MQ=255)                                                                                  aGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCt                                                  >  2:782922/1‑140 (MQ=255)                                                                                    cGAAACGGGAGCCAGTGAAAGCTGCCGCTTACCGTAACGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGc                                                <  1:269906/140‑1 (MQ=255)                                                                                      aaaCGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCg                                              <  1:728615/140‑1 (MQ=255)                                                                                                 aGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCACAGTTCATGGCCGGGTCGTGGCCGCCGGTGCCgttgtt                                   >  1:302677/1‑140 (MQ=255)                                                                                                 aGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCgttgtt                                   >  2:780383/1‑140 (MQ=255)                                                                                                          tGCGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCAAAAGTTCATCGCAAAGGCGTGGCTGCCGGTGCCGTTGTTTTCCGgcgc                          >  2:371962/1‑140 (MQ=255)                                                                                                           gCGGCTGACCGTAAGGGACGAATGGGGCGGGGATGTCGTGCATCAGGATGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGgcgcg                         <  2:369706/140‑1 (MQ=255)                                                                                                            cGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGgcgcgc                        >  1:616324/1‑140 (MQ=255)                                                                                                            cGGCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCACGACGTGGCTGCCCGTGCGGTTGTTTTCCGgcgcgc                        >  2:381605/1‑140 (MQ=255)                                                                                                             ggCTGACCGTAAGGGGCGAAGGGGGTGGGGATGCCGTTCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCt                       <  1:371962/140‑1 (MQ=255)                                                                                                             ggCTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCt                       <  1:670660/140‑1 (MQ=255)                                                                                                               cTGACCGTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGa                     >  2:169882/1‑140 (MQ=255)                                                                                                                 gACCGTAAGGGGCGAAGGGGGCGGGGATGCGGTCCATCAGGCCGGTCGACCGGCGGGCGAGGCGCGGGTTCAGGCCCCGGTTGCCAAAGTTCATCGCCAGGCCGGGGCTGCCGGGGCGGTTGTTTTCCGGGGCGCTGACg                   >  2:142154/1‑140 (MQ=255)                                                                                                                     gTAAGGGGCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAg               <  2:784774/140‑1 (MQ=255)                                                                                                                          gggCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATg          <  2:616324/140‑1 (MQ=255)                                                                                                                           ggCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGc         <  1:660442/140‑1 (MQ=255)                                                                                                                            gCGAAGGGGGCGGGGATGCCGTCCATCAGGATGGTCGAGCGGCTGGCGAGGCGCTGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGa            <  1:866448/136‑1 (MQ=255)                                                                                                                            gCGAAGGGGGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCTGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGa            >  2:866448/1‑136 (MQ=255)                                                                                                                               aaGGGGGCGGGGATGACGTCCATCAAGACCGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGtt     <  1:381605/140‑1 (MQ=255)                                                                                                                                 ggggGCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTaa   <  1:444015/140‑1 (MQ=255)                                                                                                                                  ggggCGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAg  <  1:142154/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         TTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAG  >  NC_000913/4515941‑4516209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑