Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,524,555	A→G	R152R (CGT→CGC)	yjhH ←	KpLE2 phage‑like element; putative 2‑dehydro‑3‑deoxy‑D‑pentonate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,524,555	0	A	G	100.0%	195.2 / NA	55	R152R (CGT→CGC)	yjhH	KpLE2 phage‑like element; putative 2‑dehydro‑3‑deoxy‑D‑pentonate aldolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/35);  total (20/35)

ATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATCTGCTGGTAATAGTCGTCAAGATTTCGTGGTGCGACTTTCCAGTAG  >  NC_000913/4524423‑4524687                                                                                                                                     |                                                                                                                                     aTGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGTTGTCTTTGATGCGAGCAATATTCTCGTTTTGAAGAGCCAAGCGCGTCa                                                                                                                               >  2:393566/1‑140 (MQ=255) aTGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCa                                                                                                                               >  2:784740/1‑140 (MQ=255) aTGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCa                                                                                                                               >  1:711820/1‑140 (MQ=255) aTGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCa                                                                                                                               >  1:677041/1‑140 (MQ=255)   gATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACg                                                                                                                             <  2:711820/140‑1 (MQ=255)          gTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCg                                                                                                                      <  1:784740/140‑1 (MQ=255)          gTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCg                                                                                                                      <  2:124363/140‑1 (MQ=255)          gTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCg                                                                                                                      <  2:534989/140‑1 (MQ=255)          gTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCg                                                                                                                      <  2:652206/140‑1 (MQ=255)               cGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGtt                                                                                                                 <  2:682517/140‑1 (MQ=255)                gCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGt                                                                                                                           <  1:437173/129‑1 (MQ=255)                gCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGt                                                                                                                           >  2:437173/1‑129 (MQ=255)                   gAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCTGGGTTAAGt                                                                                                             >  2:502918/1‑140 (MQ=255)                      tACCGAAAACGACGGTCGTACCAACTTAATTGTGTTGTTCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCt                                                                                                          <  1:335479/140‑1 (MQ=255)                        ccGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGa                                                                                                        <  1:519859/140‑1 (MQ=255)                         cGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGAc                                                                                                       >  1:655047/1‑140 (MQ=255)                           aaaaCGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGAccc                                                                                                     >  1:856290/1‑140 (MQ=255)                           aaaaCGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCATCACGGTTTCCGGAGTTAAGTCCTGAccc                                                                                                     >  1:398176/1‑140 (MQ=255)                             aaCGACGGGCGTACCGACTTAACTGAGTTGATCATCGGACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTACTGACCCGt                                                                                                   <  1:393566/140‑1 (MQ=255)                               cgacgGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCa                                                                                                 <  2:428542/140‑1 (MQ=255)                                  cgGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGAt                                                                                              >  1:44701/1‑140 (MQ=255)                                       gTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGa                                                                                         <  2:677041/140‑1 (MQ=255)                                          ccGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAg                                                                                      <  2:447620/140‑1 (MQ=255)                                              cTTAACTGTGTTGATCATCGTACGCAAGTGACCAAGGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGt                                                                                  <  2:290900/140‑1 (MQ=255)                                               ttAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTa                                                                                 <  1:204503/140‑1 (MQ=255)                                                tAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTAc                                                                                >  2:65637/1‑140 (MQ=255)                                                     gtgtTGATTATCGTACGCAAGTCACCAACGCTGTCTATGATGTCTTTGGTGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCGGGAAAGTTGTACAGGAt                                                                           <  2:398176/140‑1 (MQ=255)                                                     gtgtTGATCATCGTACGGAAGTAACCAACGCAGTCCATGCTGTCTTTGCTGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGAt                                                                           <  2:283183/140‑1 (MQ=255)                                                     gtgtTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGAt                                                                           <  2:856290/140‑1 (MQ=255)                                                        ttGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGAt                                                                           <  2:669933/137‑1 (MQ=255)                                                        ttGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGAt                                                                           >  1:669933/1‑137 (MQ=255)                                                         tGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCAcc                                                                       >  1:206628/1‑140 (MQ=255)                                                                         gTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTAc                                                       >  2:549546/1‑140 (MQ=255)                                                                          tGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACg                                                      >  1:796669/1‑140 (MQ=255)                                                                              cAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCg                                                  <  1:502918/140‑1 (MQ=255)                                                                              cAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCg                                                  <  1:65637/140‑1 (MQ=255)                                                                                aCGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGAt                                                <  2:655047/140‑1 (MQ=255)                                                                                aCGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGAt                                                <  2:206628/140‑1 (MQ=255)                                                                                aCGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGAt                                                <  1:178457/140‑1 (MQ=255)                                                                                  gCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATct                                              <  2:796669/140‑1 (MQ=255)                                                                                     gTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATctgct                                           >  1:597655/1‑140 (MQ=255)                                                                                        gATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATCTGCTGGt                                        <  2:21196/140‑1 (MQ=255)                                                                                          tGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATCTGCTGGTaa                                      <  1:205806/140‑1 (MQ=255)                                                                                           ggTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCg                                                  <  1:65499/127‑1 (MQ=255)                                                                                           ggTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCg                                                  >  2:65499/1‑127 (MQ=255)                                                                                            gtgtCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATCTGCTGGTAATa                                    <  1:549546/140‑1 (MQ=255)                                                                                             tgtCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATctg                                             >  2:47053/1‑130 (MQ=255)                                                                                             tgtCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATctg                                             <  1:47053/130‑1 (MQ=255)                                                                                              gtCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATCTGCTGGTAATAgt                                  <  1:619809/140‑1 (MQ=255)                                                                                                cTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATCTGCTGGTAATAgtcg                                >  2:767146/1‑140 (MQ=255)                                                                                                                   ctcGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATCTGCTGGTAATAGTCGTCAAGATTTCGTGGTGCGa             <  1:767146/140‑1 (MQ=255)                                                                                                                    tcGTTTTGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATCTGCTGGTAATAGTCGTCAAGATTTCGTGGTGCGAc            <  1:70350/140‑1 (MQ=255)                                                                                                                         ttGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATCTGCTGGTAATTGTCTTCAAGATTTCGTGGTGCGACTTTcc       >  1:311735/1‑140 (MQ=255)                                                                                                                         ttGCAGAGCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATCTGCTGGTAATAGTCGTCAAGATTTCGTGGTGCGACTTTcc       <  2:597655/140‑1 (MQ=255)                                                                                                                              gagCCAGGCGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATCTGCTGGTAATAGTCGTCAAGATTTCGTGGTGCGACTTTCCagtag  <  2:44701/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                     ATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATCTGCTGGTAATAGTCGTCAAGATTTCGTGGTGCGACTTTCCAGTAG  >  NC_000913/4524423‑4524687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑