Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,551,005	G→A	R98C (CGC→TGC)	gntP ←	fructuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,551,005	0	G	A	100.0%	177.7 / NA	52	R98C (CGC→TGC)	gntP	fructuronate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/26);  total (26/26)

TTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCGCAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTCCCCCAGCGTGTTGCCGAAGCCCGCTTTCATA  >  NC_000913/4550875‑4551141                                                                                                                                   |                                                                                                                                         ttCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCgag                                                                                                                                 >  2:103052/1‑140 (MQ=255)       aaCGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACgcgc                                                                                                                           <  2:654927/140‑1 (MQ=255)         cGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACgcgc                                                                                                                           >  2:450752/1‑138 (MQ=255)         cGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACgcgc                                                                                                                           <  1:450752/138‑1 (MQ=255)           gAATTTTAACTTCGGCAGCAATGACAATAACCAACGGCGTTAACATGACAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCcagc                                                                                                                       <  2:390422/140‑1 (MQ=255)                   gCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATg                                                                                                               <  2:667772/140‑1 (MQ=255)                     ttCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATgcg                                                                                                             <  2:655860/140‑1 (MQ=255)                       cggcggCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCt                                                                                                           <  1:826454/140‑1 (MQ=255)                                        acCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGcc                                                                                          >  2:862072/1‑140 (MQ=255)                                         ccAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACACGCCAGCAGCGGATACGCTATATGGTGAGCCGCGCCg                                                                                         >  1:268732/1‑140 (MQ=255)                                          cAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCgg                                                                                        >  1:750790/1‑140 (MQ=255)                                             cggcgCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGt                                                                                     <  2:734757/140‑1 (MQ=255)                                              ggcgCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCACCGTATGCGCTCTCTGGCGAGCCGCGCCGGAGTc                                                                                    >  2:410667/1‑140 (MQ=255)                                                   tAACATGATAAAGGCCAGTTCAAAGAACATCGCCAAACCCAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCa                                                                               <  2:277594/140‑1 (MQ=255)                                                             aaGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTGTCTGGTGGGCCACGACGGAGTTGACCATCAATTTAcc                                                                     >  1:266756/1‑140 (MQ=255)                                                                gCCACTTCATAGAACATCGCCAAACCAAAAACCAGCCCGCTGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGAt                                                                  <  2:268732/140‑1 (MQ=255)                                                                 ccACTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATg                                                                 >  1:537785/1‑140 (MQ=255)                                                                   aCTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGAc                                                               >  2:682737/1‑140 (MQ=255)                                                                    cTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGAcc                                                              >  1:641749/1‑140 (MQ=255)                                                                    cTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGAcc                                                              >  2:68478/1‑140 (MQ=255)                                                                            aaCATCGCCAGACCAGAAATCATGCTGATCATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCACACCGaa                                                      <  1:410667/140‑1 (MQ=255)                                                                            aaCATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGaa                                                      <  1:68478/140‑1 (MQ=255)                                                                            aaCATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGaa                                                      <  1:862072/140‑1 (MQ=255)                                                                            aaCATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTAGTGAGCCGAGCCAGAGGCGACCATCGATTTACCGATGACCGCACCGaa                                                      >  2:237124/1‑140 (MQ=255)                                                                              cATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAaca                                                    >  2:584673/1‑140 (MQ=255)                                                                               aTCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAacac                                                   >  1:865995/1‑140 (MQ=255)                                                                                 cGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAAcacca                                                 >  1:206965/1‑140 (MQ=255)                                                                                 cGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAAcacca                                                 <  1:584673/140‑1 (MQ=255)                                                                                 cGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAAcacca                                                 >  1:702539/1‑140 (MQ=255)                                                                                 cGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAACTTGCCGATGTCCGCACCGAAcacca                                                 >  1:409204/1‑140 (MQ=255)                                                                                 cGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAATCGACCAGCAAATTACCGATGTCCGCACCGAAcacca                                                 >  1:252153/1‑140 (MQ=255)                                                                                 cGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAACCGCGCCGAAGTCGGCCATCAATTTGCCGATGACCGCACCGAAcaccc                                                 >  2:390262/1‑139 (MQ=255)                                                                                 cGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAAACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGaa                                                      <  1:852979/135‑1 (MQ=255)                                                                                 cGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAAACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGaa                                                      >  2:852979/1‑135 (MQ=255)                                                                                               tCAGGCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGt                                   <  2:702539/140‑1 (MQ=255)                                                                                                  ggCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTcc                                >  2:842260/1‑140 (MQ=255)                                                                                                   gCCGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTccc                               >  2:824457/1‑140 (MQ=255)                                                                                                    ccGATGATAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTcccc                              <  2:794993/140‑1 (MQ=255)                                                                                                        tgatAATCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTCCCCCAGc                          >  2:497024/1‑140 (MQ=255)                                                                                                             aTCACCGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTCCCCCAGCGTGtt                     >  2:125869/1‑140 (MQ=255)                                                                                                                  cGACAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTCCCCCAGCGTGTTGCCGa                <  2:750790/140‑1 (MQ=255)                                                                                                                    aCAGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACTGCACCGAAAACCTCTATGATAGCCAGTTCCTTCAGCGTGTTGCCGaac              >  2:381642/1‑139 (MQ=255)                                                                                                                      aGCTGTACATAGCACTGACCGATACGCGCCAGCAGCGGATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTCCCCCAGCGTGTTGCCGAAGcc            <  1:405622/140‑1 (MQ=255)                                                                                                                      aGCTGTACATAGCACAGCCCGAAACGCGCCAACAGAGTACGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTCCCCCAGCGTGTTGCCGAAGcc            <  2:252153/140‑1 (MQ=255)                                                                                                                      aGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGTCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTCCCCCAGCGTGTTGCCGAAGcc            <  2:738872/140‑1 (MQ=255)                                                                                                                      aGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTCCCCCAGCGTGTTGCCGAAGcc            <  1:103052/140‑1 (MQ=255)                                                                                                                      aGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTCCCCCAGCGTGTTGCCGAAGcc            <  2:865995/140‑1 (MQ=255)                                                                                                                      aGCTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTCCCCCAGCGTGTTGCCGAAGcc            <  2:537785/140‑1 (MQ=255)                                                                                                                        cTGTACATAGCACAGCCCGAGAAGCTCCATCAGCGTACGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTCCCCCAGCGTGTTGCCGAAGCCCg          <  1:237124/140‑1 (MQ=255)                                                                                                                        cTGTACATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTCCCCCAGCGTGTTGCCGAAGCCCg          <  2:641749/140‑1 (MQ=255)                                                                                                                            aCATAGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTCCCCCAGCGTGTTGCCGAAGCCCGCttt      >  2:66819/1‑140 (MQ=255)                                                                                                                                aGCACAGACCGAGACTCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTCCCCCAGCGTGTTGCCGAAGCCCGCTTTCATa  <  1:257042/140‑1 (MQ=255)                                                                                                                                aGCACAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTCCCCCAGCGTGTTGCCGAAGCCCGCTTTCATa  <  2:743078/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         TTCAGGAACGGAATTTTAGCTTCGGCGGCAATAACAATAACCAGCGGCGCTAACATGATAAAGGCCACTTCATAGAACATCGCCAGACCAAAAATCAGGCCGATGATAATCACCGACAGCTGTACATAGCGCAGACCGAGACGCGCCAGCAGCGTATGCGCTATCTGGTGAGCCGCGCCGGAGTCGACCATCAATTTACCGATGACCGCACCGAACACCACGATGATAGCCAGTTCCCCCAGCGTGTTGCCGAAGCCCGCTTTCATA  >  NC_000913/4550875‑4551141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑