Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,582,379	G→A	L224L (CTG→TTG)	hsdM ←	type I restriction enzyme EcoKI methyltransferase component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,582,379	0	G	A	100.0%	64.2 / NA	20	L224L (CTG→TTG)	hsdM	type I restriction enzyme EcoKI methyltransferase component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/12);  total (8/12)

GGCGACAATATGCGCCTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCG  >  NC_000913/4582257‑4582513                                                                                                                           |                                                                                                                                       ggCGACAATATGCGCCTGCGGCGGGTTTTCAGCCTCGCTACCCAGAGTTTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAAACGACGGGTGCCGGGCa                                                                                                                       <  2:410476/140‑1 (MQ=255)  gCGACAATATGCGCCTTCGGCATGTTTTCACCGTCTCTATCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAAACGACGGGTGCCGGGCAc                                                                                                                      <  2:259367/140‑1 (MQ=255)         tatGCGCCTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAAACGACGGGTGCCGGGCACCAGTTCg                                                                                                               <  1:444293/140‑1 (MQ=255)               ccTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAAACGACGGGTGCCGGGCACCAGTTCGAGGCCg                                                                                                         >  1:467043/1‑140 (MQ=255)               ccTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAAACGACGGGTGCCGGGCACCAGTTCGAGGCCg                                                                                                         >  2:206201/1‑140 (MQ=255)                   cGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAAACGACGGGTGCCGGGCACCAGTTCGAGGCCGATaa                                                                                                     >  2:136403/1‑140 (MQ=255)                    ggCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAAACGACGGGTGCCGGGCACCAGTTCGAGGCCGATaaa                                                                                                    <  1:206201/140‑1 (MQ=255)                      cAGGTTTTCACCGTCGCTACCCTGAGTGTTGGCCATACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAAACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAAcg                                                                                                  <  2:332874/140‑1 (MQ=255)                        ggTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAAACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAAcgcg                                                                                                <  1:136403/140‑1 (MQ=255)                                    cGCTACCCAGAGTGTTGCCCAGATGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAAACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTgg                                                                                    <  2:30998/140‑1 (MQ=255)                                    cGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAAACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTgg                                                                                    >  2:790833/1‑140 (MQ=255)                                                          aCGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAAACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCaa                                                              >  2:84194/1‑140 (MQ=255)                                                            ggATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAAACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAgg                                                            <  1:826787/140‑1 (MQ=255)                                                            ggATTGCGCCGCCGTGGTCGAGGTTGCCTTCA‑‑ATCGTGCAGCAGGCAGTTCATCAGTGCCAAACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGgtc                                                          >  1:21917/1‑140 (MQ=255)                                                                 gcgcCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAAACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGgtcgtc                                                       <  1:468092/140‑1 (MQ=255)                                                                                aGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAAACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTg                                        <  2:467043/140‑1 (MQ=255)                                                                                                       caggcagTTCATCAGTGCCAAACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCtt                 >  1:652993/1‑140 (MQ=255)                                                                                                         ggcagTTCATCAGTGCCAAACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTtca               <  1:790833/140‑1 (MQ=255)                                                                                                              ttCATCAGTGCCAAACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTtcaatcaa          <  1:211388/140‑1 (MQ=255)                                                                                                                 atcaGTGCCAAACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCaaaaa       <  2:652993/140‑1 (MQ=255)                                                                                                                      tGCCAAACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCccg  >  1:791431/1‑140 (MQ=255)                                                                                                                           |                                                                                                                                       GGCGACAATATGCGCCTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCG  >  NC_000913/4582257‑4582513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑