Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,634,532	T→C	D260G (GAT→GGT)	rob ←	DNA‑binding transcriptional dual regulator Rob

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,634,532	0	T	C	100.0%	174.3 / NA	49	D260G (GAT→GGT)	rob	DNA‑binding transcriptional dual regulator Rob
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/19);  total (30/19)

CATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCG  >  NC_000913/4634399‑4634664                                                                                                                                      |                                                                                                                                     cATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGAc                                                                                                                                >  1:426400/1‑140 (MQ=255)          tCTGGACGCCCCTGCATAAGATTAGCTGCAGCGTTCACGTCGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGgc                                                                                                                       <  2:234530/140‑1 (MQ=255)                             gATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTGAATGCCCGGACCTTTAGGGCGCGTCAGGCTGAGCATTgg                                                                                                    >  2:337992/1‑140 (MQ=255)                             gATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTgg                                                                                                    >  1:641996/1‑140 (MQ=255)                                 aGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATg                                                                                                >  2:164639/1‑140 (MQ=255)                                   cTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACGCTGGCCTATACGGCGCCTCAGATTGAGCATTGGCATGCa                                                                                              >  2:226537/1‑140 (MQ=255)                                    tGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCAc                                                                                             <  1:91042/140‑1 (MQ=255)                                    tGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCAc                                                                                             <  2:641996/140‑1 (MQ=255)                                                gacgGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGATGAGCAGTGGCATTCACGTTCCGTATAcc                                                                                 >  1:252856/1‑140 (MQ=255)                                                 acgGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCg                                                                                <  2:28906/140‑1 (MQ=255)                                                 acgGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCg                                                                                <  1:528686/140‑1 (MQ=255)                                                  cgGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGt                                                                               <  2:146668/140‑1 (MQ=255)                                                    gATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCa                                                                             >  2:745978/1‑140 (MQ=255)                                                     aTCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGt                                                                                           >  1:14949/1‑125 (MQ=255)                                                     aTCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGt                                                                                           <  2:14949/125‑1 (MQ=255)                                                     aTCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGACAGGTTGAGCATTGGCCTGCAAGTTCCGTATACCGTCAg                                                                            >  2:334283/1‑140 (MQ=255)                                                             cagcagTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCATCAGGTTGGGCAGTGGGATGCACGTTCCGTGTACCGTCAGGATAAACt                                                                    >  2:271357/1‑140 (MQ=255)                                                               gcagTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTcc                                                                  >  1:721169/1‑140 (MQ=255)                                                                   ttCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCa                                                              >  1:117040/1‑140 (MQ=255)                                                                    tCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGCATACCGTCATGATAAACTCCTGCAc                                                             >  1:356694/1‑140 (MQ=255)                                                                       caGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGcc                                                          <  2:426400/140‑1 (MQ=255)                                                                               aTTAATTGGGCGATCTCCCGCTTTGGCATTTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCa                                                  <  1:334283/140‑1 (MQ=255)                                                                                  aaTTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGAc                                               >  1:482477/1‑140 (MQ=255)                                                                                    ttGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCt                                             >  2:767506/1‑140 (MQ=255)                                                                                    ttGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCt                                             >  2:558318/1‑140 (MQ=255)                                                                                    ttGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTAAACCGTCAGGATAAACTACTGCACGCCGGTTCCCAGACCt                                             >  2:364100/1‑140 (MQ=255)                                                                                       ggCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCa                                          >  1:29502/1‑140 (MQ=255)                                                                                       ggCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTGCCGTATACGGTCGGGAGAAACTCCTGCACGACGGTTCCCAGACCTTCa                                          >  2:222282/1‑140 (MQ=255)                                                                                       ggCGATCTCCCGCTTTGGCATCCTCCGCCGGGTAGTAGCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCa                                          <  1:271357/140‑1 (MQ=255)                                                                                       ggCGATCTCCCGCTTTGGCATCCTCCGCCAGGTAGTAACGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCa                                          <  1:244232/140‑1 (MQ=255)                                                                                        gCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCAt                                         >  2:660840/1‑140 (MQ=255)                                                                                         cGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATa                                        >  2:573400/1‑140 (MQ=255)                                                                                             ctcCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTa                                    <  1:558318/140‑1 (MQ=255)                                                                                             ctcCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTa                                    <  2:721169/140‑1 (MQ=255)                                                                                              tcCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTaa                                   >  2:203289/1‑140 (MQ=255)                                                                                                      tGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATcaca                           >  1:141545/1‑140 (MQ=255)                                                                                                      tGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATcaca                           >  1:37343/1‑140 (MQ=255)                                                                                                       ggCATCTTCTGCCGGTTAGTATCGCACAATACCCTGATCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACat                          <  1:222282/140‑1 (MQ=255)                                                                                                       ggCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGTGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACat                          >  1:852726/1‑140 (MQ=255)                                                                                                          aTCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATAtt                       <  1:745978/140‑1 (MQ=255)                                                                                                            cttctGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTcg                     <  2:141545/140‑1 (MQ=255)                                                                                                                       tagtaTCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTgcag          >  1:732516/1‑140 (MQ=255)                                                                                                                       tagtaTCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTgcag          >  2:70293/1‑140 (MQ=255)                                                                                                                             cGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCAc    <  2:29502/140‑1 (MQ=255)                                                                                                                              gCTCAATACCCTGACCTTTACGTCGCGTCAGGTTGGGCATTGGCATGCTCGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCAcc   <  2:352019/140‑1 (MQ=255)                                                                                                                               cTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTcgccg                  >  2:16464/1‑124 (MQ=255)                                                                                                                               cTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTcgccg                  <  1:16464/124‑1 (MQ=255)                                                                                                                               cTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAAGTAAACAGCACGTATTCGCCGGCCTGCAGCATCACCg  >  1:259741/1‑140 (MQ=255)                                                                                                                               cTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAAGTAAACAGCACGTAGTCGCCGCCCTGCAGCATCACCg  >  1:352019/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                     CATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCG  >  NC_000913/4634399‑4634664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑