Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	426,301	C→T	L55L (CTC→CTT)	tgt →	tRNA‑guanine transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	426,301	0	C	T	100.0%	86.1 / NA	26	L55L (CTC→CTT)	tgt	tRNA‑guanine transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/15);  total (11/15)

CGCGCACGCCGTGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCC  >  NC_000913/426167‑426428                                                                                                                                       |                                                                                                                                cgcgCACGCCGTGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGTAAATTATCCTTGGCaa                                                                                                                            <  2:179841/140‑1 (MQ=255) cgcgCACGCCGTGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCaa                                                                                                                            <  1:205407/140‑1 (MQ=255) cgcgCACGCCGTGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCaa                                                                                                                            <  1:562214/140‑1 (MQ=255)          cGTGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCa                                                                                                                   >  1:726414/1‑140 (MQ=255)             ggCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCt                                                                                                                <  2:726414/140‑1 (MQ=255)                        cTTTGATCGTGGCGTCGTGGAACCGACTTCTTTTATGCCTGTTGGCACTTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTCGCAACACCTTCCACCTGTGGCTGCGcc                                                                                                     <  1:285974/140‑1 (MQ=255)                        cTTTGATCGTGGCGTAGTGGAATCGCCTTATTTTATGACTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGcc                                                                                                     <  1:239220/140‑1 (MQ=255)                             aTCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGCCCggg                                                                                                 <  2:760790/139‑1 (MQ=255)                             aTCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGCCCggg                                                                                                 >  1:760790/1‑139 (MQ=255)                                 tGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAgg                                                                                            >  2:653092/1‑140 (MQ=255)                                              aCGCCTTGTTTTATGACTGTTGGCACCTAGGGCACCGCAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACt                                                                               <  1:236234/140‑1 (MQ=255)                                                gCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGc                                                                             >  1:690415/1‑140 (MQ=255)                                                   ttGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACg                                                                          >  2:426204/1‑140 (MQ=255)                                                   ttGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACg                                                                          >  1:588239/1‑140 (MQ=255)                                                             ccTGTTGGCACCTACTGCACCGCAAAAGGGATGACGCAGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCa                                                                <  1:323789/140‑1 (MQ=255)                                                             ccTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCa                                                                <  2:588239/140‑1 (MQ=255)                                                                    gCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGAtttt                                                         <  2:24123/140‑1 (MQ=255)                                                                         tACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCa                                                    <  1:653092/140‑1 (MQ=255)                                                                                  gTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTAt                                                       >  2:77902/1‑128 (MQ=255)                                                                                  gTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTAt                                                       <  1:77902/128‑1 (MQ=255)                                                                                           aTGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGTCGATCTGCACGATTTTTTGCACTGGAAGGGGCCGATCCt                                  >  2:287576/1‑140 (MQ=255)                                                                                           aTGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGAGCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCt                                  >  2:343296/1‑140 (MQ=255)                                                                                                  cGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCAGGGCGATCTGCACGAATATATGCAGTGGAAGGGGCCGATCCTCACCGAc                           >  2:420882/1‑140 (MQ=255)                                                                                                    gaagaaGTTGAAGCCACTGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTc                         <  2:690415/140‑1 (MQ=255)                                                                                                                     tGGCGCGCAAATTATCCTTGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCt        >  1:627327/1‑140 (MQ=255)                                                                                                                           gcAAATTATCCTTGGCAACACCCTCCACCAGTGGCTGTGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGcc  <  1:420882/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                CGCGCACGCCGTGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCC  >  NC_000913/426167‑426428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑