Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	466,851	G→A	G154G (GGC→GGT)	ybaE ←	putative protein YbaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	466,851	0	G	A	100.0%	168.4 / NA	51	G154G (GGC→GGT)	ybaE	putative protein YbaE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/28);  total (23/28)

TTTTACTGCATCGCCGTTATGCCAGTGTAGGGTTGAACGAAGATAAAAGTCCCAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGGCCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGG  >  NC_000913/466716‑466985                                                                                                                                        |                                                                                                                                       ttttACTGCATCGCCGTTATGCCAGTGTAGGGTTGAACGAAGATAAAAGTCCCAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCgg                                                                                                                                    <  2:554713/140‑1 (MQ=255)  tttACTGCATCGCCGTTATGCCAGTGTAGGGTTGAACGAAGATAAAAGTCCCAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGa                                                                                                                                   <  1:117542/140‑1 (MQ=255)  tttACTGCATCGCCGTTATGCCAGTGTAGGGTTGAACGAAGATAAAAGTCCCAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGa                                                                                                                                   <  2:78399/140‑1 (MQ=255)  tttACTGCATCGCCGCTATGCCAGTATAGTGTTGAACGAAGATAAAAGTCCCAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGa                                                                                                                                   <  1:366897/140‑1 (MQ=255)   ttACTGCATCGCCGTTATGCCAGTGTAGGGTTGAACGAAGATAAAAGTCCCAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGaa                                                                                                                                  >  2:144067/1‑140 (MQ=255)   ttACTGCATCGCCGTTATGCCAGTGTAGGGTTGAACGAAGATAAAAGTCCCAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGaa                                                                                                                                  <  2:601585/140‑1 (MQ=255)     aCTGCATCGCCGTTATGCCAGTGTAGGGTTGAACGAAGATAAAAGTCCCAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGaaaa                                                                                                                                >  2:27724/1‑140 (MQ=255)        gCATCGCCGTTATGCCAGTGTAGGGTTGAACGAAGATAAAAGTCCCAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAatat                                                                                                                             <  2:476084/140‑1 (MQ=255)          aTCGCCGTTATGCCAGTGTAGGGTTGAACGAAGATAAAAGTCCCAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCt                                                                                                                           >  2:24116/1‑140 (MQ=255)                                   gAACGAAGATAAAAGTCCCAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACg                                                                                                  >  1:688473/1‑140 (MQ=255)                                   gAACGAAGATAAAAGTCCCAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACg                                                                                                  >  1:535271/1‑140 (MQ=255)                                    aaCGAAGATAAAAGTCCCAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACgg                                                                                                 >  1:398208/1‑140 (MQ=255)                                    aaCGAAGATAAAAGTCCCAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACgg                                                                                                 >  2:117379/1‑140 (MQ=255)                                       gAAGATAAAAGTCCCAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCg                                                                                              <  1:832138/140‑1 (MQ=255)                                             aaaaGTCCCAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCaaa                                                                                        <  1:24116/140‑1 (MQ=255)                                                     cAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTgg                                                                                <  1:668537/140‑1 (MQ=255)                                                     cAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTgg                                                                                >  2:494881/1‑140 (MQ=255)                                                                    gTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAg                                                                 <  2:651980/140‑1 (MQ=255)                                                                     tAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGCGCAGAAAGCCTGGTTGTAGCGGTTCGAGc                                                                >  1:341903/1‑140 (MQ=255)                                                                      agagGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCg                                                               <  1:614287/140‑1 (MQ=255)                                                                       gagGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCgg                                                              >  1:623716/1‑140 (MQ=255)                                                                        agGTTTCCCAGTGATGCGCTAACTCGCCAATCGGGAGCTTAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCggg                                                             <  2:252051/140‑1 (MQ=255)                                                                        agGTTTCCCAGTGATACGCTAAATCACCAATCGGGCGATGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCggg                                                             <  1:265484/140‑1 (MQ=255)                                                                           tttCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGa                                                          <  2:688473/140‑1 (MQ=255)                                                                             tCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGc                                                            <  1:51475/136‑1 (MQ=255)                                                                             tCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGc                                                            >  2:51475/1‑136 (MQ=255)                                                                                  gTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAgg                                                   <  2:623716/140‑1 (MQ=255)                                                                                    gATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGa                                                 >  2:683993/1‑140 (MQ=255)                                                                                       gcgcTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATa                                              <  1:117379/140‑1 (MQ=255)                                                                                       gcgcTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATa                                              <  1:494881/140‑1 (MQ=255)                                                                                        cgcTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATAc                                             >  2:172817/1‑140 (MQ=255)                                                                                        cgcTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATAc                                             >  2:571400/1‑140 (MQ=255)                                                                                            aaaTCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCaa                                         <  1:683993/140‑1 (MQ=255)                                                                                              aTCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGTTAGTAGGGAATACGCAACg                                       <  2:535271/140‑1 (MQ=255)                                                                                                     aTCGGGCGCTGAGTACTATTATCGACGCGAGTCAGACAGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGgtgt                                <  1:417827/140‑1 (MQ=255)                                                                                                                          tCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGATTACGCAAGGTGGGTGTATCGTATTGCCATTGTCCGcc           >  1:219153/1‑140 (MQ=255)                                                                                                                          tCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTg                       <  2:73964/128‑1 (MQ=255)                                                                                                                          tCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTg                       >  1:73964/1‑128 (MQ=255)                                                                                                                          tCGAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGcc           >  1:501177/1‑140 (MQ=255)                                                                                                                            gAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCa         >  2:207146/1‑140 (MQ=255)                                                                                                                            gAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCa         >  1:69645/1‑140 (MQ=255)                                                                                                                            gAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCa         >  1:776278/1‑140 (MQ=255)                                                                                                                            gAAGCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGACAGTAGGGATTACACAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCa         >  2:291037/1‑140 (MQ=255)                                                                                                                               gCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATaa      >  1:519813/1‑140 (MQ=255)                                                                                                                               gCGGGTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATaa      <  2:776278/140‑1 (MQ=255)                                                                                                                                 gggTCAGACCGGAAAATCTCTGACCGGCGAGCTGCTGCTCAGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAAc    <  2:219153/140‑1 (MQ=255)                                                                                                                                   gTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACgg  <  1:144067/140‑1 (MQ=255)                                                                                                                                   gTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACgg  <  2:501177/140‑1 (MQ=255)                                                                                                                                   gTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACgg  >  2:427471/1‑140 (MQ=255)                                                                                                                                   gTCAGACCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACgg  <  2:69645/140‑1 (MQ=255)                                                                                                                                   gTCAGACCGGAAAATACTTGCCTGGCGAGATGCGGTTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACgg  <  2:398208/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TTTTACTGCATCGCCGTTATGCCAGTGTAGGGTTGAACGAAGATAAAAGTCCCAGCGTAACCCGTCAGTAGAGGTTTCCCAGTGATGCGCTAAATCGCCAATCGGGCGCTGAGTATTATTATCGAAGCGGGTCAGGCCGGAAAATATCTGCCCGGCGAGATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCGGGCGATAGTAGGGAATACGCAACGTGGGTGTATCGTTTTGCCATTGTCCGCCCATAAACGG  >  NC_000913/466716‑466985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑