Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 474,447 A→G F239L (TTT→CTT)  tesB ← acyl‑CoA thioesterase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913474,4470AG100.0% 161.6 / NA 46F239L (TTT→CTT) tesBacyl‑CoA thioesterase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/21);  total (25/21)

ATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGA  >  NC_000913/474310‑474571
                                                                                                                                         |                                                                                                                            
aTTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCg                                                                                                                            <  1:180461/140‑1 (MQ=255)
aTTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACACCGAGGTGATCTCCACCCTATACAGAAGCCCTTCATTCAAATTAAGCg                                                                                                                            >  2:274041/1‑140 (MQ=255)
          aCCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGaa                                                                                                                  >  1:490019/1‑140 (MQ=255)
          aCCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCCTCTCGGGTATAACACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGaa                                                                                                                  <  2:243094/140‑1 (MQ=255)
                        ggTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGt                                                                                                    <  2:196851/140‑1 (MQ=255)
                          tCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGGAGCCATTCATTCAAATAAAGCGGGCGATGGTACCACATGGAATGGTCa                                                                                                  >  2:266934/1‑140 (MQ=255)
                          tCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGa                                                                                                         >  1:571510/1‑133 (MQ=255)
                          tCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGa                                                                                                         >  1:741448/1‑133 (MQ=255)
                          tCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGa                                                                                                         <  2:571510/133‑1 (MQ=255)
                          tCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGa                                                                                                         <  2:741448/133‑1 (MQ=255)
                          tCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCa                                                                                                  >  2:597427/1‑140 (MQ=255)
                                    cAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCaa                                                                                        >  1:771766/1‑140 (MQ=255)
                                    cAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCAGTCAAATTAAGCGGGCGATGGAACCATATGGAATGGTCAATGGTGGCaa                                                                                        >  2:265015/1‑140 (MQ=255)
                                      gTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATc                                                                                      <  2:490183/140‑1 (MQ=255)
                                       tACGCCGTCTTGGGTTTAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCGAATTAAGCCGGCAATGTAACCACATAGAATGGTCAATGGTGCGAATCt                                                                                     >  2:363950/1‑140 (MQ=255)
                                                 tGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCgg                                                                           <  2:473196/140‑1 (MQ=255)
                                                  gggTATAAAACTCACCGCGCACTAATCCACGTGCGCTCGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGt                                                                          <  1:232132/140‑1 (MQ=255)
                                                       taAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCgag                                                                     <  1:460652/140‑1 (MQ=255)
                                                             tCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCGATGATGGCATTCAGAATGCCCGGTTCGAGAAAAcc                                                               >  2:256158/1‑140 (MQ=255)
                                                               aCCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGa                                                             >  1:63623/1‑140 (MQ=255)
                                                                 cgcgCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATg                                                           <  1:794816/140‑1 (MQ=255)
                                                                      acaAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTg                                                      <  2:771766/140‑1 (MQ=255)
                                                                      acaAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTg                                                      <  2:63623/140‑1 (MQ=255)
                                                                         aaGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCgg                                                   <  2:580057/140‑1 (MQ=255)
                                                                                gTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTaga                                            >  1:823500/1‑140 (MQ=255)
                                                                                          cGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCa                                  <  1:597427/140‑1 (MQ=255)
                                                                                               aGGTGCTCTCCACGCTATACAGCAGGCATTCATTCAATTTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAg                             <  1:265015/140‑1 (MQ=255)
                                                                                               aGGTGCTCTCCACGCGATACAGCAGGCATCCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAg                             <  1:266934/140‑1 (MQ=255)
                                                                                                  tGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAgg                                >  1:470436/1‑134 (MQ=255)
                                                                                                  tGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAgg                                <  2:470436/134‑1 (MQ=255)
                                                                                                         cACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAg                   <  1:614660/140‑1 (MQ=255)
                                                                                                         cACGCTATACAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGGGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAg                   >  2:614660/1‑140 (MQ=255)
                                                                                                              tataCAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTATCGGCAGGAATTTAAGATCAGAAGCg              >  1:315847/1‑140 (MQ=255)
                                                                                                                taCAGCAGCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTa            >  2:690181/1‑140 (MQ=255)
                                                                                                                  cagcagCCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAAc          <  2:490019/140‑1 (MQ=255)
                                                                                                                     cagcCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGa       >  2:139031/1‑140 (MQ=255)
                                                                                                                     cagcCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGa       >  1:742605/1‑140 (MQ=255)
                                                                                                                     cagcCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGa       >  1:818073/1‑140 (MQ=255)
                                                                                                                     cagcCATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAG‑AAACCGATGCCGGGCGGCTGGAGAACTACCGGCAAGAAGGTTAGGTCAAAAGCGTAAACGAg      >  1:484201/1‑140 (MQ=255)
                                                                                                                      agcCATTCATTCAAATTAAGCGCGCGATGGACCCAAATGAAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAg      <  2:315847/140‑1 (MQ=255)
                                                                                                                        ccATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTAGCGGGAGGAAGTTGAGATAAGAAGCGTAACCGAGCa    >  2:248106/1‑140 (MQ=255)
                                                                                                                        ccATTCATTCAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCa    >  1:659024/1‑140 (MQ=255)
                                                                                                                          attcattcAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGa  >  1:514448/1‑140 (MQ=255)
                                                                                                                          attcattcAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGa  >  1:47870/1‑140 (MQ=255)
                                                                                                                          attcattcAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGa  >  1:686959/1‑140 (MQ=255)
                                                                                                                          attcattcAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGa  <  2:659024/140‑1 (MQ=255)
                                                                                                                          attcattcAAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGa  >  2:130156/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                            
ATTACGCATCACCCCTTCCTGAACGGTCGAGGCAACCAGTACGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGA  >  NC_000913/474310‑474571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑